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- W2007062289 abstract "Bacterial protoplasts have been used to demonstrate the infectious activity of a variety of bacteriophage nucleic acids. Spizizen (1957) and Fraser, Mahler, Shug and Thomas (1957) demonstrated that protoplasts could be infected by subviral particles of the coliphage T2. The single-stranded DNA of bacteriophage ØX174 was shown to be infectious by Guthrie and Sinsheimer (1960) and Sekiguchi, Taketo and Takagi (1960). More recently, Fouace and Huppert (1962) and Knolle and Kaudewitz (1962) have reported that protoplasts can be infected by the RNA of the RNA bacteriophages f2, FH4, FH5 and ft5.The RNA of bacteriophage R17, which was isolated in this laboratory (Paranchych and Graham, 1962), also has been found to be infectious to bacterial protoplasts. However, under conditions similar to those described by Fouace et al. (1962), the infectivity yield was found to be very low (one infectious RNA unit per 108 phage). An investigation was thus carried out to find more favorable conditions for estimating infectious RNA. This communication describes an assay system in which the infectivity yield has been increased to approximately one infectious RNA unit per 105 phage." @default.
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- W2007062289 date "1960-01-01" @default.
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- W2007062289 title "An infective deoxyribonucleic acid from bacteriophage φX174" @default.
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- W2007062289 doi "https://doi.org/10.1016/0006-3002(60)91443-8" @default.
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