Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007071103> ?p ?o ?g. }
- W2007071103 endingPage "e91997" @default.
- W2007071103 startingPage "e91997" @default.
- W2007071103 abstract "Introduction Brown adipose tissue (BAT) is a potential therapeutic target to reverse obesity. The purpose of this study was to determine whether primary precursor cells isolated from human adult subcutaneous white adipose tissue (WAT) can be induced to differentiate in-vitro into adipocytes that express key markers of brown or beige adipose, and whether the expression level of such markers differs between lean and obese young adult males. Methods Adipogenic precursor cells were isolated from lean and obese individuals from subcutaneous abdominal WAT biopsies. Cells were grown to confluence, differentiated for 2.5 weeks then harvested for measurement of gene expression and UCP1 protein. Results There was no difference between groups with respect to differentiation into adipocytes, as indicated by oil red-O staining, rates of lipolysis, and expression of adipogenic genes (FABP4, PPARG). WAT genes (HOXC9, RB1) were expressed equally in the two groups. Post differentiation, the beige adipose specific genes CITED1 and CD137 were significantly increased in both groups, but classic BAT markers ZIC1 and LHX8 decreased significantly. Cell lines from both groups also equally increased post-differentiation expression of the thermogenic-responsive gene PPARGC1A (PGC-1α). UCP1 gene expression was undetectable prior to differentiation, however after differentiation both gene expression and protein content were increased in both groups and were significantly greater in cultures from lean compared with obese individuals (p<0.05). Conclusion Human subcutaneous WAT cells can be induced to attain BAT characteristics, but this capacity is reduced in WAT cells from obese individuals." @default.
- W2007071103 created "2016-06-24" @default.
- W2007071103 creator A5016445385 @default.
- W2007071103 creator A5021722939 @default.
- W2007071103 creator A5042088340 @default.
- W2007071103 creator A5042728320 @default.
- W2007071103 creator A5044257317 @default.
- W2007071103 creator A5048747672 @default.
- W2007071103 creator A5076818444 @default.
- W2007071103 creator A5080061829 @default.
- W2007071103 creator A5089411889 @default.
- W2007071103 date "2014-03-18" @default.
- W2007071103 modified "2023-09-24" @default.
- W2007071103 title "Reduced UCP-1 Content in In Vitro Differentiated Beige/Brite Adipocytes Derived from Preadipocytes of Human Subcutaneous White Adipose Tissues in Obesity" @default.
- W2007071103 cites W1558325430 @default.
- W2007071103 cites W1705611056 @default.
- W2007071103 cites W1891518777 @default.
- W2007071103 cites W1965079305 @default.
- W2007071103 cites W1970246917 @default.
- W2007071103 cites W1972836378 @default.
- W2007071103 cites W1973042766 @default.
- W2007071103 cites W1974058241 @default.
- W2007071103 cites W1979026577 @default.
- W2007071103 cites W1981243279 @default.
- W2007071103 cites W1992326632 @default.
- W2007071103 cites W1996744282 @default.
- W2007071103 cites W2005827500 @default.
- W2007071103 cites W2005946810 @default.
- W2007071103 cites W2006547120 @default.
- W2007071103 cites W2013900763 @default.
- W2007071103 cites W2014697141 @default.
- W2007071103 cites W2018806199 @default.
- W2007071103 cites W2025530025 @default.
- W2007071103 cites W2025838486 @default.
- W2007071103 cites W2026952221 @default.
- W2007071103 cites W2027753197 @default.
- W2007071103 cites W2033590944 @default.
- W2007071103 cites W2036378283 @default.
- W2007071103 cites W2039992465 @default.
- W2007071103 cites W2040328785 @default.
- W2007071103 cites W2044021940 @default.
- W2007071103 cites W2051993213 @default.
- W2007071103 cites W2060846519 @default.
- W2007071103 cites W2062600479 @default.
- W2007071103 cites W2069099066 @default.
- W2007071103 cites W2071015666 @default.
- W2007071103 cites W2080639215 @default.
- W2007071103 cites W2083602312 @default.
- W2007071103 cites W2102921035 @default.
- W2007071103 cites W2111517662 @default.
- W2007071103 cites W2112076976 @default.
- W2007071103 cites W2115313813 @default.
- W2007071103 cites W2117821842 @default.
- W2007071103 cites W2118055379 @default.
- W2007071103 cites W2121042865 @default.
- W2007071103 cites W2131762758 @default.
- W2007071103 cites W2132358997 @default.
- W2007071103 cites W2146625096 @default.
- W2007071103 cites W2147627509 @default.
- W2007071103 cites W2153081066 @default.
- W2007071103 cites W2168239899 @default.
- W2007071103 cites W2168727368 @default.
- W2007071103 cites W2337786074 @default.
- W2007071103 doi "https://doi.org/10.1371/journal.pone.0091997" @default.
- W2007071103 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3958417" @default.
- W2007071103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24642703" @default.
- W2007071103 hasPublicationYear "2014" @default.
- W2007071103 type Work @default.
- W2007071103 sameAs 2007071103 @default.
- W2007071103 citedByCount "67" @default.
- W2007071103 countsByYear W20070711032014 @default.
- W2007071103 countsByYear W20070711032015 @default.
- W2007071103 countsByYear W20070711032016 @default.
- W2007071103 countsByYear W20070711032017 @default.
- W2007071103 countsByYear W20070711032018 @default.
- W2007071103 countsByYear W20070711032019 @default.
- W2007071103 countsByYear W20070711032020 @default.
- W2007071103 countsByYear W20070711032021 @default.
- W2007071103 countsByYear W20070711032022 @default.
- W2007071103 countsByYear W20070711032023 @default.
- W2007071103 crossrefType "journal-article" @default.
- W2007071103 hasAuthorship W2007071103A5016445385 @default.
- W2007071103 hasAuthorship W2007071103A5021722939 @default.
- W2007071103 hasAuthorship W2007071103A5042088340 @default.
- W2007071103 hasAuthorship W2007071103A5042728320 @default.
- W2007071103 hasAuthorship W2007071103A5044257317 @default.
- W2007071103 hasAuthorship W2007071103A5048747672 @default.
- W2007071103 hasAuthorship W2007071103A5076818444 @default.
- W2007071103 hasAuthorship W2007071103A5080061829 @default.
- W2007071103 hasAuthorship W2007071103A5089411889 @default.
- W2007071103 hasBestOaLocation W20070711031 @default.
- W2007071103 hasConcept C104317684 @default.
- W2007071103 hasConcept C122927707 @default.
- W2007071103 hasConcept C126322002 @default.
- W2007071103 hasConcept C130241916 @default.
- W2007071103 hasConcept C134018914 @default.
- W2007071103 hasConcept C140985366 @default.
- W2007071103 hasConcept C143128242 @default.