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- W2007089603 abstract "The enzyme catechol 2,3-dioxygenase (C230) encoded by the nahH gene of plasmid NAH7 converts catechol to α-hydroxymuconic ε-semialdehyde in Pseudomonas putida. We have cloned this structural gene into vectors pUC18 and pKT240, determined the nucleotide sequence and deduced the amino acid sequence. In comparison to the gene xylE of the TOL plasmid pWWO which encodes a similar C230 enzyme [Nakai et al. J. Biol. Chem. (1983b), 2923–2928], the respective G + C contents were 55% and 57%, the nucleotide sequences 81% homologous, and the amino acid homology 85%. The flanking sequences of the two C230-coding genes also show homology. Clones of the nahH gene in Escherichia coli overproduce the protein product at least ten fold and the gene product was identified by polyacrylamide gel electrophoresis." @default.
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- W2007089603 date "1987-01-01" @default.
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- W2007089603 title "Nucleotide sequence and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL pWWO" @default.
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- W2007089603 doi "https://doi.org/10.1016/0378-1119(87)90244-7" @default.
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