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• W2007089797 abstract "ObjectiveMuch data supports the concept that there is little to no detectable new transcription during the final stages of oocyte maturation and the initial stages of early embryo development. Therefore most of the critical events at the time of fertilization and very early embryo development rely on an orchestrated program of translation of already existing mRNAs. BMPR (Bone morphogenetic protein receptor) mRNA is increased in polysome binding during oocyte maturation. Thus, BMP signaling may be important during oocyte maturation and early embryo development, since these events are dependent on the oocyte translational program of pre-formed maternal mRNAs. We sought to assess whether activation or blockade of BMPR would affect in vitro fertilization outcomes.DesignAn in vitro study using murine oocytes.Materials and MethodsMurine oocyte microarray data were previously generated. QPCR was used to validate all microarray data and to assess embryonic genome activation (EGA). BMPR signaling pathways were assessed by Western Blot to phospho-SMAD proteins and immunofluorescence. Murine IVF was performed under standard conditions.ResultsBMPR is significantly increased in translation during oocyte maturation. There is increased signaling to phospho-SMAD when BMPR is activated by exogenous BMP-2 in GV versus MII oocytes (2.1 fold versus 6 fold p <0.05). IVF performed under 4 different conditions [control; BMP-2 ; BML-275 (a specific competitive antagonist of BMP signaling); and BMP-2 + BML-275] showed improved fertilization rates with BMP-2 (59% ; 76% ; 57% 55%, respectively p <0.001) as well as a trend to improved blastulation rates. EGA as assessed by QPCR performed on 2 cell embryos revealed significant differences in early EGA transcripts among the groups.ConclusionBMPR signaling in the oocyte during the peri-fertilization period improves fertilization rates and affects early embryo development as assessed by embryonic genome activation, but has no significant effect on blastulation. Assessment of oocyte receptors which are increased in translation during oocyte maturation can provide a valuable understanding of signaling pathways important for oocyte competence and may provide a means to improve IVF outcomes. ObjectiveMuch data supports the concept that there is little to no detectable new transcription during the final stages of oocyte maturation and the initial stages of early embryo development. Therefore most of the critical events at the time of fertilization and very early embryo development rely on an orchestrated program of translation of already existing mRNAs. BMPR (Bone morphogenetic protein receptor) mRNA is increased in polysome binding during oocyte maturation. Thus, BMP signaling may be important during oocyte maturation and early embryo development, since these events are dependent on the oocyte translational program of pre-formed maternal mRNAs. We sought to assess whether activation or blockade of BMPR would affect in vitro fertilization outcomes. Much data supports the concept that there is little to no detectable new transcription during the final stages of oocyte maturation and the initial stages of early embryo development. Therefore most of the critical events at the time of fertilization and very early embryo development rely on an orchestrated program of translation of already existing mRNAs. BMPR (Bone morphogenetic protein receptor) mRNA is increased in polysome binding during oocyte maturation. Thus, BMP signaling may be important during oocyte maturation and early embryo development, since these events are dependent on the oocyte translational program of pre-formed maternal mRNAs. We sought to assess whether activation or blockade of BMPR would affect in vitro fertilization outcomes. DesignAn in vitro study using murine oocytes. An in vitro study using murine oocytes. Materials and MethodsMurine oocyte microarray data were previously generated. QPCR was used to validate all microarray data and to assess embryonic genome activation (EGA). BMPR signaling pathways were assessed by Western Blot to phospho-SMAD proteins and immunofluorescence. Murine IVF was performed under standard conditions. Murine oocyte microarray data were previously generated. QPCR was used to validate all microarray data and to assess embryonic genome activation (EGA). BMPR signaling pathways were assessed by Western Blot to phospho-SMAD proteins and immunofluorescence. Murine IVF was performed under standard conditions. ResultsBMPR is significantly increased in translation during oocyte maturation. There is increased signaling to phospho-SMAD when BMPR is activated by exogenous BMP-2 in GV versus MII oocytes (2.1 fold versus 6 fold p <0.05). IVF performed under 4 different conditions [control; BMP-2 ; BML-275 (a specific competitive antagonist of BMP signaling); and BMP-2 + BML-275] showed improved fertilization rates with BMP-2 (59% ; 76% ; 57% 55%, respectively p <0.001) as well as a trend to improved blastulation rates. EGA as assessed by QPCR performed on 2 cell embryos revealed significant differences in early EGA transcripts among the groups. BMPR is significantly increased in translation during oocyte maturation. There is increased signaling to phospho-SMAD when BMPR is activated by exogenous BMP-2 in GV versus MII oocytes (2.1 fold versus 6 fold p <0.05). IVF performed under 4 different conditions [control; BMP-2 ; BML-275 (a specific competitive antagonist of BMP signaling); and BMP-2 + BML-275] showed improved fertilization rates with BMP-2 (59% ; 76% ; 57% 55%, respectively p <0.001) as well as a trend to improved blastulation rates. EGA as assessed by QPCR performed on 2 cell embryos revealed significant differences in early EGA transcripts among the groups. ConclusionBMPR signaling in the oocyte during the peri-fertilization period improves fertilization rates and affects early embryo development as assessed by embryonic genome activation, but has no significant effect on blastulation. Assessment of oocyte receptors which are increased in translation during oocyte maturation can provide a valuable understanding of signaling pathways important for oocyte competence and may provide a means to improve IVF outcomes. BMPR signaling in the oocyte during the peri-fertilization period improves fertilization rates and affects early embryo development as assessed by embryonic genome activation, but has no significant effect on blastulation. Assessment of oocyte receptors which are increased in translation during oocyte maturation can provide a valuable understanding of signaling pathways important for oocyte competence and may provide a means to improve IVF outcomes." @default.
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• W2007089797 date "2014-09-01" @default.
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• W2007089797 title "Special research presentation: bmpr signaling in the mammalian oocyte improves fertilization and influences early embryo development" @default.
• W2007089797 doi "https://doi.org/10.1016/j.fertnstert.2014.07.402" @default.
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