Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007095194> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2007095194 endingPage "1026" @default.
- W2007095194 startingPage "1017" @default.
- W2007095194 abstract "Chlamydiae are obligate intracellular bacteria that replicate within a non-acidified vacuole, termed an inclusion. To identify chlamydial proteins that are unique to the intracellular phase of the life cycle, a lambda expression library of Chlamydia psittaci DNA was differentially screened with convalescent antisera from infected guinea pigs and antisera directed at formalin-fixed purified chlamydial elementary bodies (EBs). One library clone was identified that harboured two open reading frames (ORFs) with coding potential for similar-sized proteins of ≈20 kDa. These proteins were subsequently termed IncB and IncC. Sequencing of the cloned insert revealed a strong Escherichia coli-like promoter sequence immediately upstream of incB and a 36 nt intergenic region between the ORFs. Sequence analysis of the region upstream of incB and incC revealed two ORFs that had strong homologies to an amino acid transporter and a sodium-dependent transporter. Immunoblotting with antisera directed at IncB or IncC demonstrated that these proteins are present in C. psittaci-infected HeLa cells but are absent or below the level of detection in purified EBs. Reverse transcriptase-polymerase chain reactions provided evidence that incB and incC are transcribed in an operon. Immunofluorescence microscopy demonstrated that IncB and IncC are each localized to the inclusion membrane of infected cells. No primary sequence similarity is evident between IncA, IncB or IncC, but each contains a large hydrophobic domain of similar size and character as in IncA. Analysis of the recently completed C. trachomatis serovar D genome database has revealed C. trachomatis ORFs encoding homologues to incB and incC, indicating that these genes are conserved among the chlamydiae." @default.
- W2007095194 created "2016-06-24" @default.
- W2007095194 creator A5051283258 @default.
- W2007095194 creator A5072384168 @default.
- W2007095194 creator A5081751244 @default.
- W2007095194 date "1998-06-01" @default.
- W2007095194 modified "2023-10-18" @default.
- W2007095194 title "Tandem genes of Chlamydia psittaci that encode proteins localized to the inclusion membrane" @default.
- W2007095194 cites W1526950041 @default.
- W2007095194 cites W1674177946 @default.
- W2007095194 cites W1884556878 @default.
- W2007095194 cites W1890701869 @default.
- W2007095194 cites W1918492041 @default.
- W2007095194 cites W1975304761 @default.
- W2007095194 cites W1982557947 @default.
- W2007095194 cites W1985947029 @default.
- W2007095194 cites W1991417567 @default.
- W2007095194 cites W1993350361 @default.
- W2007095194 cites W2014358885 @default.
- W2007095194 cites W2046246341 @default.
- W2007095194 cites W2052966812 @default.
- W2007095194 cites W2084310228 @default.
- W2007095194 cites W2084894679 @default.
- W2007095194 cites W2104416424 @default.
- W2007095194 cites W2110331556 @default.
- W2007095194 cites W2114489404 @default.
- W2007095194 cites W2114765873 @default.
- W2007095194 cites W2117411464 @default.
- W2007095194 cites W2119784689 @default.
- W2007095194 cites W2124492750 @default.
- W2007095194 cites W2129551826 @default.
- W2007095194 cites W2145382965 @default.
- W2007095194 cites W2148913589 @default.
- W2007095194 cites W2163094102 @default.
- W2007095194 cites W2167149210 @default.
- W2007095194 cites W4249180073 @default.
- W2007095194 cites W968722387 @default.
- W2007095194 doi "https://doi.org/10.1046/j.1365-2958.1998.00867.x" @default.
- W2007095194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9663687" @default.
- W2007095194 hasPublicationYear "1998" @default.
- W2007095194 type Work @default.
- W2007095194 sameAs 2007095194 @default.
- W2007095194 citedByCount "84" @default.
- W2007095194 countsByYear W20070951942012 @default.
- W2007095194 countsByYear W20070951942014 @default.
- W2007095194 countsByYear W20070951942015 @default.
- W2007095194 countsByYear W20070951942016 @default.
- W2007095194 countsByYear W20070951942017 @default.
- W2007095194 countsByYear W20070951942018 @default.
- W2007095194 countsByYear W20070951942019 @default.
- W2007095194 countsByYear W20070951942023 @default.
- W2007095194 crossrefType "journal-article" @default.
- W2007095194 hasAuthorship W2007095194A5051283258 @default.
- W2007095194 hasAuthorship W2007095194A5072384168 @default.
- W2007095194 hasAuthorship W2007095194A5081751244 @default.
- W2007095194 hasBestOaLocation W20070951941 @default.
- W2007095194 hasConcept C104317684 @default.
- W2007095194 hasConcept C153911025 @default.
- W2007095194 hasConcept C159047783 @default.
- W2007095194 hasConcept C167625842 @default.
- W2007095194 hasConcept C2777391075 @default.
- W2007095194 hasConcept C2777667758 @default.
- W2007095194 hasConcept C2780122209 @default.
- W2007095194 hasConcept C2780530800 @default.
- W2007095194 hasConcept C47289529 @default.
- W2007095194 hasConcept C54355233 @default.
- W2007095194 hasConcept C86803240 @default.
- W2007095194 hasConceptScore W2007095194C104317684 @default.
- W2007095194 hasConceptScore W2007095194C153911025 @default.
- W2007095194 hasConceptScore W2007095194C159047783 @default.
- W2007095194 hasConceptScore W2007095194C167625842 @default.
- W2007095194 hasConceptScore W2007095194C2777391075 @default.
- W2007095194 hasConceptScore W2007095194C2777667758 @default.
- W2007095194 hasConceptScore W2007095194C2780122209 @default.
- W2007095194 hasConceptScore W2007095194C2780530800 @default.
- W2007095194 hasConceptScore W2007095194C47289529 @default.
- W2007095194 hasConceptScore W2007095194C54355233 @default.
- W2007095194 hasConceptScore W2007095194C86803240 @default.
- W2007095194 hasIssue "5" @default.
- W2007095194 hasLocation W20070951941 @default.
- W2007095194 hasLocation W20070951942 @default.
- W2007095194 hasOpenAccess W2007095194 @default.
- W2007095194 hasPrimaryLocation W20070951941 @default.
- W2007095194 hasRelatedWork W1976390578 @default.
- W2007095194 hasRelatedWork W1978801575 @default.
- W2007095194 hasRelatedWork W2017335389 @default.
- W2007095194 hasRelatedWork W2017477982 @default.
- W2007095194 hasRelatedWork W2029252138 @default.
- W2007095194 hasRelatedWork W2072439611 @default.
- W2007095194 hasRelatedWork W2101899141 @default.
- W2007095194 hasRelatedWork W2358544099 @default.
- W2007095194 hasRelatedWork W3031046715 @default.
- W2007095194 hasRelatedWork W4306784270 @default.
- W2007095194 hasVolume "28" @default.
- W2007095194 isParatext "false" @default.
- W2007095194 isRetracted "false" @default.
- W2007095194 magId "2007095194" @default.
- W2007095194 workType "article" @default.