Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007100556> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2007100556 endingPage "403" @default.
- W2007100556 startingPage "399" @default.
- W2007100556 abstract "BACKGROUND: Microbiologic agents such as variola virus (VAR) are very attractive for terrorism. As a result of international collaboration under the WHO eradication campaign, smallpox was declared eradicated in 1980. Therefore, the immunization programs were discontinued worldwide. Because most people are now immunologically naive, VAR is considered to be a potential threat agent or bioterrorist weapon. Real‐time polymerase chain reaction (PCR) followed by melting analysis was developed for fast and safe analysis and allows differentiation of VAR from other orthopoxviruses (OPVs) like vaccinia or camelpox virus. STUDY DESIGN AND METHODS: A RealArt TM Orthopox LC PCR kit (Artus GmbH) was used to amplify OPV sequences from blood donor samples. A total of 31,500 blood donor samples were tested in minipools of up to 96 samples. To evaluate the sensitivity of the assay, routine donor minipools (90 ± 6 samples per pool) were spiked with vaccinia virus used as positive control. RESULTS: Specificity was 100 percent because none of 31,500 blood donors was positive for the presence OPV. The detection limit of the assay was 10.6 copies per PCR procedure. Therefore, a sensitivity of 1590 copies per mL was calculated. Overall, 0.28 percent of test results had to be considered invalid owing to negative internal controls. CONCLUSION: The RealArt Orthopox LC PCR kit enables reliable detection of OPV DNA in viremic blood donor samples, even at the beginning of the disease when patients present minor clinical symptoms, and could be implemented in our routine screening procedure immediately. Thus, the assay could potentially help to prevent dispersion of viral agents by blood transfusion in case of bioterrorism." @default.
- W2007100556 created "2016-06-24" @default.
- W2007100556 creator A5027258903 @default.
- W2007100556 creator A5045146362 @default.
- W2007100556 creator A5070710999 @default.
- W2007100556 creator A5081633372 @default.
- W2007100556 creator A5088797832 @default.
- W2007100556 date "2005-03-01" @default.
- W2007100556 modified "2023-10-15" @default.
- W2007100556 title "Nucleic acid test screening of blood donors for orthopoxviruses can potentially prevent dispersion of viral agents in case of bioterrorism" @default.
- W2007100556 cites W1525716180 @default.
- W2007100556 cites W1966089781 @default.
- W2007100556 cites W1972543711 @default.
- W2007100556 cites W1973474680 @default.
- W2007100556 cites W1998952510 @default.
- W2007100556 cites W2051889688 @default.
- W2007100556 cites W2073354185 @default.
- W2007100556 cites W2073650386 @default.
- W2007100556 cites W2073918417 @default.
- W2007100556 cites W2081146564 @default.
- W2007100556 cites W2104086033 @default.
- W2007100556 cites W2120718338 @default.
- W2007100556 cites W2129132016 @default.
- W2007100556 cites W2166848776 @default.
- W2007100556 cites W2169992778 @default.
- W2007100556 cites W2170517330 @default.
- W2007100556 cites W2411432596 @default.
- W2007100556 cites W4322697591 @default.
- W2007100556 doi "https://doi.org/10.1111/j.1537-2995.2005.04242.x" @default.
- W2007100556 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15752158" @default.
- W2007100556 hasPublicationYear "2005" @default.
- W2007100556 type Work @default.
- W2007100556 sameAs 2007100556 @default.
- W2007100556 citedByCount "15" @default.
- W2007100556 countsByYear W20071005562012 @default.
- W2007100556 countsByYear W20071005562014 @default.
- W2007100556 countsByYear W20071005562015 @default.
- W2007100556 countsByYear W20071005562017 @default.
- W2007100556 countsByYear W20071005562022 @default.
- W2007100556 crossrefType "journal-article" @default.
- W2007100556 hasAuthorship W2007100556A5027258903 @default.
- W2007100556 hasAuthorship W2007100556A5045146362 @default.
- W2007100556 hasAuthorship W2007100556A5070710999 @default.
- W2007100556 hasAuthorship W2007100556A5081633372 @default.
- W2007100556 hasAuthorship W2007100556A5088797832 @default.
- W2007100556 hasConcept C104317684 @default.
- W2007100556 hasConcept C159047783 @default.
- W2007100556 hasConcept C22070199 @default.
- W2007100556 hasConcept C2522874641 @default.
- W2007100556 hasConcept C2778417375 @default.
- W2007100556 hasConcept C2779094796 @default.
- W2007100556 hasConcept C2781356689 @default.
- W2007100556 hasConcept C2909788366 @default.
- W2007100556 hasConcept C40767141 @default.
- W2007100556 hasConcept C49105822 @default.
- W2007100556 hasConcept C55493867 @default.
- W2007100556 hasConcept C71924100 @default.
- W2007100556 hasConcept C86803240 @default.
- W2007100556 hasConceptScore W2007100556C104317684 @default.
- W2007100556 hasConceptScore W2007100556C159047783 @default.
- W2007100556 hasConceptScore W2007100556C22070199 @default.
- W2007100556 hasConceptScore W2007100556C2522874641 @default.
- W2007100556 hasConceptScore W2007100556C2778417375 @default.
- W2007100556 hasConceptScore W2007100556C2779094796 @default.
- W2007100556 hasConceptScore W2007100556C2781356689 @default.
- W2007100556 hasConceptScore W2007100556C2909788366 @default.
- W2007100556 hasConceptScore W2007100556C40767141 @default.
- W2007100556 hasConceptScore W2007100556C49105822 @default.
- W2007100556 hasConceptScore W2007100556C55493867 @default.
- W2007100556 hasConceptScore W2007100556C71924100 @default.
- W2007100556 hasConceptScore W2007100556C86803240 @default.
- W2007100556 hasIssue "3" @default.
- W2007100556 hasLocation W20071005561 @default.
- W2007100556 hasLocation W20071005562 @default.
- W2007100556 hasOpenAccess W2007100556 @default.
- W2007100556 hasPrimaryLocation W20071005561 @default.
- W2007100556 hasRelatedWork W1905216348 @default.
- W2007100556 hasRelatedWork W1963688935 @default.
- W2007100556 hasRelatedWork W1969834418 @default.
- W2007100556 hasRelatedWork W1973660939 @default.
- W2007100556 hasRelatedWork W1982871514 @default.
- W2007100556 hasRelatedWork W2046508631 @default.
- W2007100556 hasRelatedWork W2170974892 @default.
- W2007100556 hasRelatedWork W2395455967 @default.
- W2007100556 hasRelatedWork W4231915716 @default.
- W2007100556 hasRelatedWork W4236022722 @default.
- W2007100556 hasVolume "45" @default.
- W2007100556 isParatext "false" @default.
- W2007100556 isRetracted "false" @default.
- W2007100556 magId "2007100556" @default.
- W2007100556 workType "article" @default.