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- W2007106350 abstract "APC gene mutations play a role in the initiation step of colorectal carcinogenesis in both familial adenomatous polyposis (FAP) and non-FAP patients. Almost all of the APC mutations are nonsense or frameshift mutations, which truncate the APC protein and are thought to inactivate normal APC function. We show a novel method for detecting nonsense and frameshift APC gene mutations by using Saccharomyces cerevisiae. Polymerase chain reaction (PCR)-amplified APC fragments are cloned directly into yeast expression vectors in vivo, and the yeast expresses a hemagglutinin epitope (HA)-tagged APC peptide. When an APC fragment contains a nonsense or frameshift mutation, HA-tagged truncating APC peptide can be detected by Western blotting using an anti-HA antibody. We identified both germ-line and somatic APC mutations in patients with FAP and non-FAP colorectal tumors, respectively. This method, called the yeast-based protein truncation test (YPTT), is simple and fairly cheap, and it can be applied to any genes that are inactivated by protein truncating mutations. Genes Chromosomes Cancer 21:290–297, 1998. © 1998 Wiley-Liss, Inc." @default.
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- W2007106350 date "1998-04-01" @default.
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- W2007106350 title "Detection ofAPC mutations by a yeast-based protein truncation test (YPTT)" @default.
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- W2007106350 doi "https://doi.org/10.1002/(sici)1098-2264(199804)21:4<290::aid-gcc2>3.0.co;2-u" @default.
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