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- W2007107966 abstract "A stable isotope dilution assay for the major urinary metabolite of prostaglandins E1 and E2 (PGE-M) was developed. After extraction and purification, the compound was converted to the dimethyl ester bis(O-methyloxime)-trimethylsilyl ether derivative and quantified with the 13C-labeled dimethyl ester analog as internal standard. The interassay coefficient of variation was 0.8%, and the intraassay coefficient of variation ranged from 0.9 to 1.7%. The recovery of authentic, underivatized PGE-M added to urine was 99.5 +/- 3.2% (mean +/- SE, N = 9). The equation of the regression curve correlating the amounts added and recovered was Y = 1.05X - 5.84, with R = 0.998. The level of excreted metabolite in healthy male subjects was 13.86 +/- 1.46 micrograms/24 h (N = 24), in good agreement with data from other sources." @default.
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- W2007107966 date "1987-11-01" @default.
- W2007107966 modified "2023-09-25" @default.
- W2007107966 title "Stable isotope dilution assay for prostaglandin E metabolite: 24-Hour urinary output in healthy male subjects" @default.
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- W2007107966 doi "https://doi.org/10.1016/0003-2697(87)90149-7" @default.
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