FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007125645> ?p ?o ?g. }

• W2007125645 endingPage "261" @default.
• W2007125645 startingPage "251" @default.
• W2007125645 abstract "The N-carbamoyl-d-amino-acid amidohydrolase (D-NCAase) is used on an industrial scale for the production of d-amino acids. The crystal structure of D-NCAase was solved by multiple isomorphous replacement with anomalous scattering using xenon and gold derivatives, and refined to 1.95 Å resolution, to an R-factor of 18.6 %. The crystal structure shows a four-layer α/β fold with two six-stranded β sheets packed on either side by two α helices. One exterior layer faces the solvent, whereas the other one is buried and involved in the tight intersubunit contacts. A long C-terminal fragment extends from a monomer to a site near a dyad axis, and associates with another monomer to form a small and hydrophobic cavity, where a xenon atom can bind. Site-directed mutagenesis of His129, His144 and His215 revealed strict geometric requirements of these conserved residues to maintain a stable conformation of a putative catalytic cleft. A region located within this cleft involving Cys172, Glu47, and Lys127 is proposed for D-NCAase catalysis and is similar to the Cys-Asp-Lys site of N-carbamoylsarcosine amidohydrolase. The homologous active-site framework of these enzymes with distinct structures suggests convergent evolution of a common catalytic mechanism." @default.
• W2007125645 created "2016-06-24" @default.
• W2007125645 creator A5006822373 @default.
• W2007125645 creator A5057178901 @default.
• W2007125645 creator A5080830696 @default.
• W2007125645 creator A5083477983 @default.
• W2007125645 date "2001-02-01" @default.
• W2007125645 modified "2023-10-03" @default.
• W2007125645 title "Crystal structure and site-directed mutagenesis studies of N-carbamoyl-d-amino-acid amidohydrolase from Agrobacterium radiobacter reveals a homotetramer and insight into a catalytic cleft11Edited by R. Huber" @default.
• W2007125645 cites W1520730837 @default.
• W2007125645 cites W1536566156 @default.
• W2007125645 cites W1539796472 @default.
• W2007125645 cites W1591680814 @default.
• W2007125645 cites W1959500134 @default.
• W2007125645 cites W1968329307 @default.
• W2007125645 cites W1973795201 @default.
• W2007125645 cites W1975460230 @default.
• W2007125645 cites W1975577039 @default.
• W2007125645 cites W1986191025 @default.
• W2007125645 cites W1986397685 @default.
• W2007125645 cites W1990721673 @default.
• W2007125645 cites W1996607909 @default.
• W2007125645 cites W2001641653 @default.
• W2007125645 cites W2013083986 @default.
• W2007125645 cites W2013776698 @default.
• W2007125645 cites W2022365595 @default.
• W2007125645 cites W2028231353 @default.
• W2007125645 cites W2038840577 @default.
• W2007125645 cites W2042471904 @default.
• W2007125645 cites W2044430464 @default.
• W2007125645 cites W2052448379 @default.
• W2007125645 cites W2066743520 @default.
• W2007125645 cites W2067601423 @default.
• W2007125645 cites W2068411938 @default.
• W2007125645 cites W2078248419 @default.
• W2007125645 cites W2079974886 @default.
• W2007125645 cites W2081861554 @default.
• W2007125645 cites W2084575458 @default.
• W2007125645 cites W2090133370 @default.
• W2007125645 cites W2095511528 @default.
• W2007125645 cites W2096943217 @default.
• W2007125645 cites W2099022831 @default.
• W2007125645 cites W2101446086 @default.
• W2007125645 cites W2157698257 @default.
• W2007125645 cites W2160327918 @default.
• W2007125645 cites W2888511473 @default.
• W2007125645 doi "https://doi.org/10.1006/jmbi.2000.4380" @default.
• W2007125645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11237598" @default.
• W2007125645 hasPublicationYear "2001" @default.
• W2007125645 type Work @default.
• W2007125645 sameAs 2007125645 @default.
• W2007125645 citedByCount "75" @default.
• W2007125645 countsByYear W20071256452012 @default.
• W2007125645 countsByYear W20071256452013 @default.
• W2007125645 countsByYear W20071256452014 @default.
• W2007125645 countsByYear W20071256452016 @default.
• W2007125645 countsByYear W20071256452017 @default.
• W2007125645 countsByYear W20071256452018 @default.
• W2007125645 countsByYear W20071256452019 @default.
• W2007125645 countsByYear W20071256452020 @default.
• W2007125645 countsByYear W20071256452021 @default.
• W2007125645 countsByYear W20071256452022 @default.
• W2007125645 countsByYear W20071256452023 @default.
• W2007125645 crossrefType "journal-article" @default.
• W2007125645 hasAuthorship W2007125645A5006822373 @default.
• W2007125645 hasAuthorship W2007125645A5057178901 @default.
• W2007125645 hasAuthorship W2007125645A5080830696 @default.
• W2007125645 hasAuthorship W2007125645A5083477983 @default.
• W2007125645 hasBestOaLocation W20071256451 @default.
• W2007125645 hasConcept C103408237 @default.
• W2007125645 hasConcept C104292427 @default.
• W2007125645 hasConcept C104317684 @default.
• W2007125645 hasConcept C112247285 @default.
• W2007125645 hasConcept C115624301 @default.
• W2007125645 hasConcept C143065580 @default.
• W2007125645 hasConcept C16318435 @default.
• W2007125645 hasConcept C167625842 @default.
• W2007125645 hasConcept C181199279 @default.
• W2007125645 hasConcept C185592680 @default.
• W2007125645 hasConcept C2778481663 @default.
• W2007125645 hasConcept C2779485144 @default.
• W2007125645 hasConcept C2780120296 @default.
• W2007125645 hasConcept C47701112 @default.
• W2007125645 hasConcept C515207424 @default.
• W2007125645 hasConcept C55493867 @default.
• W2007125645 hasConcept C71240020 @default.
• W2007125645 hasConcept C8010536 @default.
• W2007125645 hasConceptScore W2007125645C103408237 @default.
• W2007125645 hasConceptScore W2007125645C104292427 @default.
• W2007125645 hasConceptScore W2007125645C104317684 @default.
• W2007125645 hasConceptScore W2007125645C112247285 @default.
• W2007125645 hasConceptScore W2007125645C115624301 @default.
• W2007125645 hasConceptScore W2007125645C143065580 @default.
• W2007125645 hasConceptScore W2007125645C16318435 @default.
• W2007125645 hasConceptScore W2007125645C167625842 @default.
• W2007125645 hasConceptScore W2007125645C181199279 @default.
• W2007125645 hasConceptScore W2007125645C185592680 @default.
• W2007125645 hasConceptScore W2007125645C2778481663 @default.

• next