Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007155218> ?p ?o ?g. }
- W2007155218 endingPage "2012" @default.
- W2007155218 startingPage "2006" @default.
- W2007155218 abstract "The first application of microchip electrophoresis with laser-induced fluorescence (MCE-LIF) detection to simultaneously determine glutathione (GSH) and hydrogen peroxide (H2O2) in mitochondria was described. Organoselenium probe Rh-Se-2 and bis(p-methylbenzenesulfonate)dichlorofluorescein (FS) synthesized in our laboratory were utilized as fluorescent probes for GSH and H2O2, respectively. Rh-Se-2, which is nonfluorescent, reacts with GSH to produce rhodamine 110 (Rh110) with high quantum yield. Similarly, nonfluorescent FS reacts with H2O2 and produces dichlorofluorescein (DCF) accompanied by drastic fluorescence enhancement. Both probes exhibit good sensitivity toward their respective target molecule determination. Fast, simple, and sensitive determination of GSH and H2O2 was realized within 37 s using a running buffer of 50 mM mannitol, 40 mM HEPES (pH 7.4), and an electric field of 360 V/cm for separation. The linear ranges of the method were 3.3 × 10−9−1.0 × 10−7 M/2.9 × 10−7−1.0 × 10−4 M and 2.7 × 10−9−4.0 × 10−7 M with detection limits (signal-to-noise ratio = 3) of 1.3 nM (0.16 amol) and 1.0 nM (0.12 amol) for GSH and H2O2, respectively. The relative standard deviations (RSDs) of migration time and peak area were less than 1.0% and 4.0%, respectively. The MCE-LIF assay was utilized to investigate the levels of GSH and H2O2 in mitochondria isolated from HepG2 cells and were found to be 2.01 ± 0.21 mM and 5.36 ± 0.45 μM, respectively. The method was further extended to observe situations of the two species in mitochondria of HepG2 cells experiencing cell apoptosis that were induced by doxorubicin and photodynamic therapy (PDT)." @default.
- W2007155218 created "2016-06-24" @default.
- W2007155218 creator A5014700415 @default.
- W2007155218 creator A5040312877 @default.
- W2007155218 creator A5056101043 @default.
- W2007155218 creator A5057193330 @default.
- W2007155218 creator A5063046811 @default.
- W2007155218 creator A5064226113 @default.
- W2007155218 creator A5076857313 @default.
- W2007155218 creator A5079871249 @default.
- W2007155218 creator A5090789682 @default.
- W2007155218 date "2010-02-09" @default.
- W2007155218 modified "2023-09-24" @default.
- W2007155218 title "Potent Method for the Simultaneous Determination of Glutathione and Hydrogen Peroxide in Mitochondrial Compartments of Apoptotic Cells with Microchip Electrophoresis-Laser Induced Fluorescence" @default.
- W2007155218 cites W1482813056 @default.
- W2007155218 cites W1968351069 @default.
- W2007155218 cites W1970007512 @default.
- W2007155218 cites W1975113323 @default.
- W2007155218 cites W1975668301 @default.
- W2007155218 cites W1986568749 @default.
- W2007155218 cites W1992956362 @default.
- W2007155218 cites W1993999089 @default.
- W2007155218 cites W1994220134 @default.
- W2007155218 cites W1997329503 @default.
- W2007155218 cites W1997953414 @default.
- W2007155218 cites W2023714162 @default.
- W2007155218 cites W2026875029 @default.
- W2007155218 cites W2029057704 @default.
- W2007155218 cites W2029376735 @default.
- W2007155218 cites W2036758684 @default.
- W2007155218 cites W2040014117 @default.
- W2007155218 cites W2051193966 @default.
- W2007155218 cites W2060697601 @default.
- W2007155218 cites W2063250806 @default.
- W2007155218 cites W2065123184 @default.
- W2007155218 cites W2068047791 @default.
- W2007155218 cites W2078339670 @default.
- W2007155218 cites W2078394930 @default.
- W2007155218 cites W2081872634 @default.
- W2007155218 cites W2087894638 @default.
- W2007155218 cites W2089700857 @default.
- W2007155218 cites W2094078251 @default.
- W2007155218 cites W2111374354 @default.
- W2007155218 cites W2131960328 @default.
- W2007155218 cites W2151704374 @default.
- W2007155218 cites W2152242012 @default.
- W2007155218 cites W2152826259 @default.
- W2007155218 cites W2155527615 @default.
- W2007155218 cites W2158720649 @default.
- W2007155218 cites W2161990266 @default.
- W2007155218 cites W2166077025 @default.
- W2007155218 cites W2313491323 @default.
- W2007155218 cites W4245899006 @default.
- W2007155218 cites W51229851 @default.
- W2007155218 doi "https://doi.org/10.1021/ac902741r" @default.
- W2007155218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20143832" @default.
- W2007155218 hasPublicationYear "2010" @default.
- W2007155218 type Work @default.
- W2007155218 sameAs 2007155218 @default.
- W2007155218 citedByCount "42" @default.
- W2007155218 countsByYear W20071552182012 @default.
- W2007155218 countsByYear W20071552182013 @default.
- W2007155218 countsByYear W20071552182014 @default.
- W2007155218 countsByYear W20071552182015 @default.
- W2007155218 countsByYear W20071552182016 @default.
- W2007155218 countsByYear W20071552182017 @default.
- W2007155218 countsByYear W20071552182018 @default.
- W2007155218 countsByYear W20071552182019 @default.
- W2007155218 countsByYear W20071552182020 @default.
- W2007155218 countsByYear W20071552182022 @default.
- W2007155218 countsByYear W20071552182023 @default.
- W2007155218 crossrefType "journal-article" @default.
- W2007155218 hasAuthorship W2007155218A5014700415 @default.
- W2007155218 hasAuthorship W2007155218A5040312877 @default.
- W2007155218 hasAuthorship W2007155218A5056101043 @default.
- W2007155218 hasAuthorship W2007155218A5057193330 @default.
- W2007155218 hasAuthorship W2007155218A5063046811 @default.
- W2007155218 hasAuthorship W2007155218A5064226113 @default.
- W2007155218 hasAuthorship W2007155218A5076857313 @default.
- W2007155218 hasAuthorship W2007155218A5079871249 @default.
- W2007155218 hasAuthorship W2007155218A5090789682 @default.
- W2007155218 hasConcept C113196181 @default.
- W2007155218 hasConcept C119128265 @default.
- W2007155218 hasConcept C121332964 @default.
- W2007155218 hasConcept C13965031 @default.
- W2007155218 hasConcept C181199279 @default.
- W2007155218 hasConcept C185592680 @default.
- W2007155218 hasConcept C2779916640 @default.
- W2007155218 hasConcept C2779964046 @default.
- W2007155218 hasConcept C43617362 @default.
- W2007155218 hasConcept C48349386 @default.
- W2007155218 hasConcept C533411734 @default.
- W2007155218 hasConcept C538909803 @default.
- W2007155218 hasConcept C55493867 @default.
- W2007155218 hasConcept C62520636 @default.
- W2007155218 hasConcept C91881484 @default.
- W2007155218 hasConceptScore W2007155218C113196181 @default.
- W2007155218 hasConceptScore W2007155218C119128265 @default.