FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007171135> ?p ?o ?g. }

• W2007171135 endingPage "5247" @default.
• W2007171135 startingPage "5239" @default.
• W2007171135 abstract "ABSTRACT Quinolone antibacterial drugs such as nalidixic acid target DNA gyrase in Escherichia coli . These inhibitors bind to and stabilize a normally transient covalent protein-DNA intermediate in the gyrase reaction cycle, referred to as the cleavage complex. Stabilization of the cleavage complex is necessary but not sufficient for cell killing—cytotoxicity apparently results from the conversion of cleavage complexes into overt DNA breaks by an as-yet-unknown mechanism(s). Quinolone treatment induces the bacterial SOS response in a RecBC-dependent manner, arguing that cleavage complexes are somehow converted into double-stranded breaks. However, the only proteins known to be required for SOS induction by nalidixic acid are RecA and RecBC. In hopes of identifying additional proteins involved in the cytotoxic response to nalidixic acid, we screened for E. coli mutants specifically deficient in SOS induction upon nalidixic acid treatment by using a dinD :: lacZ reporter construct. From a collection of SOS partially constitutive mutants with disruptions of 47 different genes, we found that dnaQ insertion mutants are specifically deficient in the SOS response to nalidixic acid. dnaQ encodes DNA polymerase III ε subunit, the proofreading subunit of the replicative polymerase. The deficient response to nalidixic acid was rescued by the presence of the wild-type dnaQ gene, confirming involvement of the ε subunit. To further characterize the SOS deficiency of dnaQ mutants, we analyzed the expression of several additional SOS genes in response to nalidixic acid using real-time PCR. A subset of SOS genes lost their response to nalidixic acid in the dnaQ mutant strain, while two tested SOS genes ( recA and recN ) continued to exhibit induction. These results argue that the replication complex plays a role in modulating the SOS response to nalidixic acid and that the response is more complex than a simple on/off switch." @default.
• W2007171135 created "2016-06-24" @default.
• W2007171135 creator A5038101718 @default.
• W2007171135 creator A5052036668 @default.
• W2007171135 creator A5058525552 @default.
• W2007171135 creator A5076037748 @default.
• W2007171135 date "2008-08-01" @default.
• W2007171135 modified "2023-10-16" @default.
• W2007171135 title "The ε Subunit of DNA Polymerase III Is Involved in the Nalidixic Acid-Induced SOS Response in <i>Escherichia coli</i>" @default.
• W2007171135 cites W1479708140 @default.
• W2007171135 cites W1497274060 @default.
• W2007171135 cites W1508943098 @default.
• W2007171135 cites W1549465097 @default.
• W2007171135 cites W1568597106 @default.
• W2007171135 cites W1592458258 @default.
• W2007171135 cites W1604704982 @default.
• W2007171135 cites W1701315331 @default.
• W2007171135 cites W1894978028 @default.
• W2007171135 cites W1910370106 @default.
• W2007171135 cites W1941246143 @default.
• W2007171135 cites W1976300710 @default.
• W2007171135 cites W1978171553 @default.
• W2007171135 cites W1978895557 @default.
• W2007171135 cites W1981176937 @default.
• W2007171135 cites W1982692787 @default.
• W2007171135 cites W1984090080 @default.
• W2007171135 cites W1984829844 @default.
• W2007171135 cites W2006128589 @default.
• W2007171135 cites W2008320236 @default.
• W2007171135 cites W2011700794 @default.
• W2007171135 cites W2027256377 @default.
• W2007171135 cites W2031645882 @default.
• W2007171135 cites W2032262899 @default.
• W2007171135 cites W2056884132 @default.
• W2007171135 cites W2059572321 @default.
• W2007171135 cites W2063127584 @default.
• W2007171135 cites W2064234883 @default.
• W2007171135 cites W2065118224 @default.
• W2007171135 cites W2081105498 @default.
• W2007171135 cites W2086076127 @default.
• W2007171135 cites W2089647775 @default.
• W2007171135 cites W2090037733 @default.
• W2007171135 cites W2096262477 @default.
• W2007171135 cites W2096359570 @default.
• W2007171135 cites W2097038081 @default.
• W2007171135 cites W2101859984 @default.
• W2007171135 cites W2101994201 @default.
• W2007171135 cites W2102589447 @default.
• W2007171135 cites W2106889520 @default.
• W2007171135 cites W2107419622 @default.
• W2007171135 cites W2121460811 @default.
• W2007171135 cites W2124624490 @default.
• W2007171135 cites W2125947686 @default.
• W2007171135 cites W2132697669 @default.
• W2007171135 cites W2134692706 @default.
• W2007171135 cites W2134894209 @default.
• W2007171135 cites W2136965916 @default.
• W2007171135 cites W2145001911 @default.
• W2007171135 cites W2151938785 @default.
• W2007171135 cites W2153145491 @default.
• W2007171135 cites W2412673113 @default.
• W2007171135 doi "https://doi.org/10.1128/jb.00173-08" @default.
• W2007171135 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2493257" @default.
• W2007171135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18539731" @default.
• W2007171135 hasPublicationYear "2008" @default.
• W2007171135 type Work @default.
• W2007171135 sameAs 2007171135 @default.
• W2007171135 citedByCount "18" @default.
• W2007171135 countsByYear W20071711352012 @default.
• W2007171135 countsByYear W20071711352013 @default.
• W2007171135 countsByYear W20071711352014 @default.
• W2007171135 countsByYear W20071711352015 @default.
• W2007171135 countsByYear W20071711352016 @default.
• W2007171135 countsByYear W20071711352021 @default.
• W2007171135 countsByYear W20071711352023 @default.
• W2007171135 crossrefType "journal-article" @default.
• W2007171135 hasAuthorship W2007171135A5038101718 @default.
• W2007171135 hasAuthorship W2007171135A5052036668 @default.
• W2007171135 hasAuthorship W2007171135A5058525552 @default.
• W2007171135 hasAuthorship W2007171135A5076037748 @default.
• W2007171135 hasBestOaLocation W20071711352 @default.
• W2007171135 hasConcept C104317684 @default.
• W2007171135 hasConcept C143065580 @default.
• W2007171135 hasConcept C150194340 @default.
• W2007171135 hasConcept C153911025 @default.
• W2007171135 hasConcept C158448853 @default.
• W2007171135 hasConcept C167125095 @default.
• W2007171135 hasConcept C2775876089 @default.
• W2007171135 hasConcept C2780852648 @default.
• W2007171135 hasConcept C413212 @default.
• W2007171135 hasConcept C547475151 @default.
• W2007171135 hasConcept C552990157 @default.
• W2007171135 hasConcept C55493867 @default.
• W2007171135 hasConcept C73573662 @default.
• W2007171135 hasConcept C86803240 @default.
• W2007171135 hasConceptScore W2007171135C104317684 @default.
• W2007171135 hasConceptScore W2007171135C143065580 @default.
• W2007171135 hasConceptScore W2007171135C150194340 @default.

• next