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• W2007179464 abstract "In vivo microperifbsion and micropuncture were used to study tubule protein synthesis and proluminal secretion by the male reproductive tract in vivo. Somniferous and caput and cauda epididymal tubules were perihsed for 3 h. with [35S]-methionine. Perifused interstitial fluid (IF), lumen fluid (LF), and tubule extract (TE) were collected. Proteins were separated by SDS-PAGE, and autoradiograms were developed. Trichloroacetic acid precipitable proteins in each fluid were determined and a protein synthesis index (PSI) was calculated. PSI values demonstrated that the cauda epididymis synthesized less protein in vivo than did either seminiferous or caput tubules. Seminiferous tubules synthesized and secreted into the tubule lumen a relatively constant panel of proteins. Epididymal tubules synthesized and secreted proteins in a region-specific manner. In the caput epididymis the most prominent secreted bands were consistent with the heavy and light chains of epididymal clusterin. In the cauda epididymis, the most prominent synthesized and secreted protein was a 25 kDa protein consistent with the protein D. The above approach to studying protein synthesis and secretion will allow direct study of the physiological and path physiological effects on this important epithelial hnction in vim" @default.
• W2007179464 created "2016-06-24" @default.
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• W2007179464 date "1994-08-01" @default.
• W2007179464 modified "2023-09-27" @default.
• W2007179464 title "Assessment of protein synthesis and secretion by rat seminiferous and epididymal tubules in vivo" @default.
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• W2007179464 doi "https://doi.org/10.1111/j.1365-2605.1994.tb01244.x" @default.
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