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- W2007186364 abstract "An efficient methodology for the determination of carbohydrate content in soils, employing acid hydrolysis and subsequent capillary electrophoresis analysis (CE), is here described. Polysaccharides present in soil samples were hydrolyzed, at 100 degrees C during 4h, to their monosaccharide form, by addition of 2moldm(-3) trifluoroacetic acid (TFA) directly to soil. The resulting monosaccharides were then quantitatively derivatized with 4-aminobenzoic acid ethyl ester, via reductive amination with sodium cyanoborohydride, and separated by CE, coupled to an UV-vis diode array set at 300nm. Separation electrolyte consisted of 5.0x10(-2)moldm(-3) sodium tetraborate buffer (pH 10.2) and 6x10(-3)moldm(-3) sodium dodecylsulphate. A 78cm long capillary with an internal and external diameter of 75 and 375mum, respectively, was used and separation performed at 16 degrees C, with an applied voltage of 30kV. Quantification was undertaken using ribose as the internal standard. As an application example, carbohydrate composition (w/w) of a farmyard manure fertilized soil was found to vary between 0.0045+/-0.0003% (glucose) and 0.0267+/-0.0002% (arabinose) of the total soil content. Xylose, rhamnose, mannose, fucose and galactose content were also studied in the present work." @default.
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- W2007186364 date "2007-04-15" @default.
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- W2007186364 title "Development and application of a capillary electrophoresis based method for the assessment of monosaccharide in soil using acid hydrolysis" @default.
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- W2007186364 doi "https://doi.org/10.1016/j.talanta.2006.10.009" @default.
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