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- W2007198417 abstract "Summary Electrophoretically-separated and purified large and small (L and S) subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from Euglena gracilis strain Z were used to generate polyclonal antibodies in rabbits. The monospecific antibodies (immunoglobulin G fractions from blood sera) were used in two different radioimmunoassays to show for the first time the patterns of accumulation of the two rubisco subunits during the greening of dark-grown Euglena exposed to white light. The subunits were detected in dark-grown cells. Following light-exposure, the nuclear-coded cytoplasmically-synthesized S subunit started to accumulate immediately whereas the chloroplastic-encoded and -synthesized L subunit began to accumulate only after 3 to 8 h. After its initial accumulation, S then remained at a constant concentration, while L continued to accumulate. During late greening, both subunits further accumulated. The differential accumulation of the two subunits, especially during early greening, correlates with the finding of a differential accumulation of cytoplasmic versus chloroplastic polysomes following light-stimulation of dark-grown Euglena ( Avadhani and Freyssinet, 1989 )" @default.
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- W2007198417 date "1992-09-01" @default.
- W2007198417 modified "2023-09-23" @default.
- W2007198417 title "Accumulation of the Large and Small Subunits of Ribulose-1,5-Bisphosphate-Carboxylase/Oxygenase during Greening in Euglena gracilis as Determined by Radioimmunoassay" @default.
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- W2007198417 doi "https://doi.org/10.1016/s0176-1617(11)80819-9" @default.
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