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- W2007207628 abstract "The reduction of adenine nucleotides at the mercury electrode in neutral buffered media (phosphate, diphosphate and citrate buffers) was studied by means of potential step generation at approx. −1.75 V (versus s.c.e.) followed by linear sweep reversal. The product of AMP reduction was characterized by h.p.l.c. and spectroscopic measurements (U.V. absorption and fluorescence). The reduction of AMP, ADP, ATP, d-AMP, cAMP, ADPR, Apa, 3′(2)′-AMP, N(6)-carboxymethyl-AMP and N(6)-[(6-aminohexyl)carbamoylmethyl]-AMP yields the corresponding dihydroadenine nucleotides which give the anodic voltammetric peak at about 0.0 V versus s.c.e. Enhanced hydrophobicity of nucleotides results in a higher yield (d-AMP > AMP), whereas enhanced negative charge on the phosphate groups causes the lowering of the anodic peak of the reduction product (AMP > ADP > ATP). No anodic response occurred with 2′-AMP, N(1)-carboxymethyl-AMP, poly-r-A, poly-r-A-G, N(1)-methyladenine, NADH, NADPH, ε-AMP and ε-ATP." @default.
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- W2007207628 date "1986-06-01" @default.
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- W2007207628 title "900 — Reduction of adenine nucleotides at the mercury electrode" @default.
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- W2007207628 doi "https://doi.org/10.1016/0302-4598(86)85021-8" @default.
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