FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007228350> ?p ?o ?g. }

• W2007228350 endingPage "57" @default.
• W2007228350 startingPage "47" @default.
• W2007228350 abstract "d-2-Hydroxyisocaproic acid dehydrogenase (d-HicDH) from Lactobacillus casei was purified and partially sequenced. A 65-mer oligodeoxyribonucleotide probe corresponding to the N-terminal 23 amino acids was synthesized and a physical map was made of the genomic region which hybridized most strongly. A strongly hybridising restriction fragment was highly purified and eventually cloned at low frequency in pBR322. The original clones spontaneously produced d-HicDH at about 0.05 % of total protein and showed viability problems in that 10- to 12-h growth-lag periods occurred after diluting stationary cultures into fresh medium. Subcloning into pGEM3 plasmids for sequencing with concomitant ExoIII deletion led to clones which no longer exhibited the growth inhibition characteristics but now made d-HicDH as 3 to 5% of total protein. Subcloning downstream from a double pLpR promoter in plasmid pJLA601 gave a highly inducible clone that builds large inclusion bodies of largely denatured d-HicDH. The gene transcript was mapped for L. casei and Escherichia coli hosts. The promoter, terminator and Shine-Dalgamo sequence are functional in both organisms. The gene encodes a protein subunit of 38 kDa, whereby 67% of the sequence could be checked by correlation with partial peptide sequences from the original enzyme. So far no Lactobacillus gene has been found to utilize the Arg codons AGG and AGA." @default.
• W2007228350 created "2016-06-24" @default.
• W2007228350 creator A5000095647 @default.
• W2007228350 creator A5003866939 @default.
• W2007228350 creator A5026919378 @default.
• W2007228350 creator A5031454564 @default.
• W2007228350 creator A5050281664 @default.
• W2007228350 creator A5061288075 @default.
• W2007228350 date "1989-05-01" @default.
• W2007228350 modified "2023-10-10" @default.
• W2007228350 title "Cloning, sequencing and expression in Escherichia coli of the d-2-hydroxyisocaproate dehydrogenase gene of Lactobacillus casei" @default.
• W2007228350 cites W1494923358 @default.
• W2007228350 cites W1505823785 @default.
• W2007228350 cites W1524513887 @default.
• W2007228350 cites W1603629403 @default.
• W2007228350 cites W1978134609 @default.
• W2007228350 cites W1983418718 @default.
• W2007228350 cites W1985224958 @default.
• W2007228350 cites W1986725603 @default.
• W2007228350 cites W1989783048 @default.
• W2007228350 cites W1991710558 @default.
• W2007228350 cites W1993937897 @default.
• W2007228350 cites W2006413114 @default.
• W2007228350 cites W2008633253 @default.
• W2007228350 cites W2019410656 @default.
• W2007228350 cites W2020204869 @default.
• W2007228350 cites W2024979313 @default.
• W2007228350 cites W2025450446 @default.
• W2007228350 cites W2032462421 @default.
• W2007228350 cites W2043743724 @default.
• W2007228350 cites W2047903962 @default.
• W2007228350 cites W2049635421 @default.
• W2007228350 cites W2049695588 @default.
• W2007228350 cites W2054423281 @default.
• W2007228350 cites W2059890422 @default.
• W2007228350 cites W2061799513 @default.
• W2007228350 cites W2063450941 @default.
• W2007228350 cites W2063873649 @default.
• W2007228350 cites W2074887246 @default.
• W2007228350 cites W2079418823 @default.
• W2007228350 cites W2080100789 @default.
• W2007228350 cites W2084633195 @default.
• W2007228350 cites W2084813203 @default.
• W2007228350 cites W2089048416 @default.
• W2007228350 cites W2095450147 @default.
• W2007228350 cites W2100837269 @default.
• W2007228350 cites W2120657153 @default.
• W2007228350 cites W2128538049 @default.
• W2007228350 cites W2132417094 @default.
• W2007228350 cites W2154882115 @default.
• W2007228350 cites W2160979138 @default.
• W2007228350 cites W2173684331 @default.
• W2007228350 cites W2346337789 @default.
• W2007228350 cites W2885454903 @default.
• W2007228350 cites W326716566 @default.
• W2007228350 cites W4296765338 @default.
• W2007228350 cites W55503133 @default.
• W2007228350 doi "https://doi.org/10.1016/0378-1119(89)90313-2" @default.
• W2007228350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2504649" @default.
• W2007228350 hasPublicationYear "1989" @default.
• W2007228350 type Work @default.
• W2007228350 sameAs 2007228350 @default.
• W2007228350 citedByCount "82" @default.
• W2007228350 countsByYear W20072283502013 @default.
• W2007228350 countsByYear W20072283502014 @default.
• W2007228350 countsByYear W20072283502015 @default.
• W2007228350 countsByYear W20072283502016 @default.
• W2007228350 countsByYear W20072283502017 @default.
• W2007228350 countsByYear W20072283502018 @default.
• W2007228350 crossrefType "journal-article" @default.
• W2007228350 hasAuthorship W2007228350A5000095647 @default.
• W2007228350 hasAuthorship W2007228350A5003866939 @default.
• W2007228350 hasAuthorship W2007228350A5026919378 @default.
• W2007228350 hasAuthorship W2007228350A5031454564 @default.
• W2007228350 hasAuthorship W2007228350A5050281664 @default.
• W2007228350 hasAuthorship W2007228350A5061288075 @default.
• W2007228350 hasConcept C104317684 @default.
• W2007228350 hasConcept C116403925 @default.
• W2007228350 hasConcept C121332964 @default.
• W2007228350 hasConcept C1276947 @default.
• W2007228350 hasConcept C128319531 @default.
• W2007228350 hasConcept C153911025 @default.
• W2007228350 hasConcept C163005120 @default.
• W2007228350 hasConcept C167625842 @default.
• W2007228350 hasConcept C170673058 @default.
• W2007228350 hasConcept C178046730 @default.
• W2007228350 hasConcept C19924922 @default.
• W2007228350 hasConcept C205225487 @default.
• W2007228350 hasConcept C22744801 @default.
• W2007228350 hasConcept C2776662696 @default.
• W2007228350 hasConcept C523546767 @default.
• W2007228350 hasConcept C54355233 @default.
• W2007228350 hasConcept C547475151 @default.
• W2007228350 hasConcept C86803240 @default.
• W2007228350 hasConceptScore W2007228350C104317684 @default.
• W2007228350 hasConceptScore W2007228350C116403925 @default.
• W2007228350 hasConceptScore W2007228350C121332964 @default.
• W2007228350 hasConceptScore W2007228350C1276947 @default.

• next