Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007239162> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2007239162 abstract "LmrP, a Major Facilitator Superfamily (MFS) multidrug transporter from Lactococcus lactis, acts a secondary antiporter, catalysing the extrusion of a large spectrum of hydrophobic drugs by dissipating a proton gradient. This implies that it has evolved to bind and export a series of structurally diverse cytotoxic substances from within the membrane, in contrast to the majority of MFS transporters that are specialized in the transport of a single soluble substrate, captured from the aqueous medium. Its transport mechanism could thus potentially differ from the alternating access model of the lactose permease that currently stands as a paradigm for all MFS transporters. Using Electron Paramagnetic Resonance spectroscopy on a library of cysteine mutants, labeled with either one or two spin probes, we aim at mapping the conformational dynamics of LmrP during its transport cycle. We are using accessibility patterns and intramolecular distance changes to understand the conformational rearrangements involved in the energization and subsequent substrate recognition and extrusion mechanisms of the transporter. Preliminary measurements on a first set of mutants show profound structural changes upon ligand binding or protonation of key acidic residues. In particular, we observe a significant conformational rearrangement of helix VIII upon substrate binding, suggesting that the interface between the N- and C-terminal halves could be key in the controlled access of drugs to the substrate binding pocket. These findings could shed light on the mechanistical divergence with MFS transporters recognizing soluble substrates from the extracellular medium. Based on these first results, we present a blueprint of the catalytic cycle of LmrP." @default.
- W2007239162 created "2016-06-24" @default.
- W2007239162 creator A5010620081 @default.
- W2007239162 creator A5031154891 @default.
- W2007239162 creator A5040786512 @default.
- W2007239162 creator A5044762631 @default.
- W2007239162 creator A5047928375 @default.
- W2007239162 creator A5059881003 @default.
- W2007239162 creator A5060600820 @default.
- W2007239162 date "2012-01-01" @default.
- W2007239162 modified "2023-09-29" @default.
- W2007239162 title "Studying the Conformational Cycle of the Secondary Multidrug Transporter LmrP by EPR Spectroscopy" @default.
- W2007239162 doi "https://doi.org/10.1016/j.bpj.2011.11.3593" @default.
- W2007239162 hasPublicationYear "2012" @default.
- W2007239162 type Work @default.
- W2007239162 sameAs 2007239162 @default.
- W2007239162 citedByCount "1" @default.
- W2007239162 countsByYear W20072391622016 @default.
- W2007239162 crossrefType "journal-article" @default.
- W2007239162 hasAuthorship W2007239162A5010620081 @default.
- W2007239162 hasAuthorship W2007239162A5031154891 @default.
- W2007239162 hasAuthorship W2007239162A5040786512 @default.
- W2007239162 hasAuthorship W2007239162A5044762631 @default.
- W2007239162 hasAuthorship W2007239162A5047928375 @default.
- W2007239162 hasAuthorship W2007239162A5059881003 @default.
- W2007239162 hasAuthorship W2007239162A5060600820 @default.
- W2007239162 hasBestOaLocation W20072391621 @default.
- W2007239162 hasConcept C104317684 @default.
- W2007239162 hasConcept C120405084 @default.
- W2007239162 hasConcept C12554922 @default.
- W2007239162 hasConcept C149011108 @default.
- W2007239162 hasConcept C177462420 @default.
- W2007239162 hasConcept C185592680 @default.
- W2007239162 hasConcept C2777339483 @default.
- W2007239162 hasConcept C41625074 @default.
- W2007239162 hasConcept C55493867 @default.
- W2007239162 hasConcept C57181701 @default.
- W2007239162 hasConcept C58598135 @default.
- W2007239162 hasConcept C62465871 @default.
- W2007239162 hasConcept C71240020 @default.
- W2007239162 hasConcept C86803240 @default.
- W2007239162 hasConceptScore W2007239162C104317684 @default.
- W2007239162 hasConceptScore W2007239162C120405084 @default.
- W2007239162 hasConceptScore W2007239162C12554922 @default.
- W2007239162 hasConceptScore W2007239162C149011108 @default.
- W2007239162 hasConceptScore W2007239162C177462420 @default.
- W2007239162 hasConceptScore W2007239162C185592680 @default.
- W2007239162 hasConceptScore W2007239162C2777339483 @default.
- W2007239162 hasConceptScore W2007239162C41625074 @default.
- W2007239162 hasConceptScore W2007239162C55493867 @default.
- W2007239162 hasConceptScore W2007239162C57181701 @default.
- W2007239162 hasConceptScore W2007239162C58598135 @default.
- W2007239162 hasConceptScore W2007239162C62465871 @default.
- W2007239162 hasConceptScore W2007239162C71240020 @default.
- W2007239162 hasConceptScore W2007239162C86803240 @default.
- W2007239162 hasLocation W20072391621 @default.
- W2007239162 hasOpenAccess W2007239162 @default.
- W2007239162 hasPrimaryLocation W20072391621 @default.
- W2007239162 hasRelatedWork W1626261382 @default.
- W2007239162 hasRelatedWork W1700585615 @default.
- W2007239162 hasRelatedWork W1750500963 @default.
- W2007239162 hasRelatedWork W1976034243 @default.
- W2007239162 hasRelatedWork W2048482150 @default.
- W2007239162 hasRelatedWork W2077559792 @default.
- W2007239162 hasRelatedWork W2103369433 @default.
- W2007239162 hasRelatedWork W2118966803 @default.
- W2007239162 hasRelatedWork W2141876587 @default.
- W2007239162 hasRelatedWork W2325213441 @default.
- W2007239162 hasRelatedWork W2412827727 @default.
- W2007239162 hasRelatedWork W2556748637 @default.
- W2007239162 hasRelatedWork W2560508032 @default.
- W2007239162 hasRelatedWork W2793343697 @default.
- W2007239162 hasRelatedWork W2944700662 @default.
- W2007239162 hasRelatedWork W2998466113 @default.
- W2007239162 hasRelatedWork W3015531816 @default.
- W2007239162 hasRelatedWork W3093594691 @default.
- W2007239162 hasRelatedWork W49589729 @default.
- W2007239162 hasRelatedWork W2081869792 @default.
- W2007239162 isParatext "false" @default.
- W2007239162 isRetracted "false" @default.
- W2007239162 magId "2007239162" @default.
- W2007239162 workType "article" @default.