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- W2007247578 abstract "Data are presented which indicate that tissue cultures of higher plants release enzymes into the medium upon which they are grown. Some of the enzyme released by the tissues appears to be bound to the cell wall. Release of indoleacetic acid oxidase (IAA oxidase) and peroxidase is enhanced when the tissues are soaked in 0. 05 M CaCl2. Cell wall preparations of the tissues when incubated with 0. 05 M CaCl2 release more IAA oxidase and acid phosphatase than do the intact tissues. Thus, it would appear that the cell walls bind the enzymes during the homogenizing procedure and the calcium ions probably serve to release the enzymes from the binding sites. One of the most interesting facts uncovered during this study is the demonstration of the release of IAA oxidase by all 10 of the tissues investigated when the tissues are soaked in 0. 05 M CaCl2. It is tentatively suggested that the release of enzymes by the tissues may serve to modify the nutrient medium to affect the growth of the tissues." @default.
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- W2007247578 date "1963-01-01" @default.
- W2007247578 modified "2023-09-27" @default.
- W2007247578 title "Release of enzymes by plant tissue cultures" @default.
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- W2007247578 doi "https://doi.org/10.1016/0024-3205(63)90037-7" @default.
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