Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007253244> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2007253244 endingPage "8534" @default.
- W2007253244 startingPage "8530" @default.
- W2007253244 abstract "Recently, a protein called kinesin was described, which is capable of inducing movement of inert particles along microtubules. To purify this protein from bovine brain, we used the ability of kinesin to bind to taxol-stabilized microtubules in the presence of inorganic tripolyphosphate. The brain kinesin preparation contained one major polypeptide of 135 kDa and four minor polypeptides of 45-70 kDa. The minor polypeptides were eluted from a gel-permeation chromatography column at the same position as the major component. All the polypeptides of the preparation were capable of binding to the microtubules under identical conditions. The kinesin molecule is most probably a complex of these polypeptides. Brain kinesin had a very low ATPase activity (0.06-0.08 mumol X min-1 X mg-1 in 3 mM Mg2+ at pH 6.7). ATPase activity was strongly stimulated by microtubules (Vmax = 4.6 mumol per min per mg of kinesin). Microtubule-activated kinesin ATPase had a Km for ATP between 10 and 12 X 10(-6) M and a Kapp for microtubules (i.e., polymerized tubulin concentration required for a half-maximal activation) of 12-14 X 10(-6) M. Kinesin had a significant ATPase activity even without microtubules if 2 mM Ca2+ was substituted for Mg2+ (Vmax = 1.6 mumol X min-1 X mg-1; Km = 800 X 10(-6) M). Kinesin is therefore a mechanochemical ATPase that is activated by microtubules." @default.
- W2007253244 created "2016-06-24" @default.
- W2007253244 creator A5026735577 @default.
- W2007253244 creator A5033899417 @default.
- W2007253244 date "1986-11-01" @default.
- W2007253244 modified "2023-10-16" @default.
- W2007253244 title "Bovine brain kinesin is a microtubule-activated ATPase." @default.
- W2007253244 cites W1130073019 @default.
- W2007253244 cites W1775749144 @default.
- W2007253244 cites W1965675171 @default.
- W2007253244 cites W1975040221 @default.
- W2007253244 cites W1993762028 @default.
- W2007253244 cites W2012141654 @default.
- W2007253244 cites W2014636427 @default.
- W2007253244 cites W2018289835 @default.
- W2007253244 cites W2020270282 @default.
- W2007253244 cites W2026145805 @default.
- W2007253244 cites W2028704064 @default.
- W2007253244 cites W2034992618 @default.
- W2007253244 cites W2036698502 @default.
- W2007253244 cites W2082631326 @default.
- W2007253244 cites W2100837269 @default.
- W2007253244 cites W2427606473 @default.
- W2007253244 doi "https://doi.org/10.1073/pnas.83.22.8530" @default.
- W2007253244 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/386964" @default.
- W2007253244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2946042" @default.
- W2007253244 hasPublicationYear "1986" @default.
- W2007253244 type Work @default.
- W2007253244 sameAs 2007253244 @default.
- W2007253244 citedByCount "191" @default.
- W2007253244 countsByYear W20072532442012 @default.
- W2007253244 countsByYear W20072532442015 @default.
- W2007253244 countsByYear W20072532442016 @default.
- W2007253244 countsByYear W20072532442017 @default.
- W2007253244 countsByYear W20072532442018 @default.
- W2007253244 countsByYear W20072532442020 @default.
- W2007253244 countsByYear W20072532442021 @default.
- W2007253244 countsByYear W20072532442022 @default.
- W2007253244 crossrefType "journal-article" @default.
- W2007253244 hasAuthorship W2007253244A5026735577 @default.
- W2007253244 hasAuthorship W2007253244A5033899417 @default.
- W2007253244 hasBestOaLocation W20072532442 @default.
- W2007253244 hasConcept C12554922 @default.
- W2007253244 hasConcept C181199279 @default.
- W2007253244 hasConcept C185592680 @default.
- W2007253244 hasConcept C20418707 @default.
- W2007253244 hasConcept C23265538 @default.
- W2007253244 hasConcept C55493867 @default.
- W2007253244 hasConcept C59006786 @default.
- W2007253244 hasConcept C61081601 @default.
- W2007253244 hasConcept C84425145 @default.
- W2007253244 hasConcept C86803240 @default.
- W2007253244 hasConcept C93126451 @default.
- W2007253244 hasConcept C95444343 @default.
- W2007253244 hasConceptScore W2007253244C12554922 @default.
- W2007253244 hasConceptScore W2007253244C181199279 @default.
- W2007253244 hasConceptScore W2007253244C185592680 @default.
- W2007253244 hasConceptScore W2007253244C20418707 @default.
- W2007253244 hasConceptScore W2007253244C23265538 @default.
- W2007253244 hasConceptScore W2007253244C55493867 @default.
- W2007253244 hasConceptScore W2007253244C59006786 @default.
- W2007253244 hasConceptScore W2007253244C61081601 @default.
- W2007253244 hasConceptScore W2007253244C84425145 @default.
- W2007253244 hasConceptScore W2007253244C86803240 @default.
- W2007253244 hasConceptScore W2007253244C93126451 @default.
- W2007253244 hasConceptScore W2007253244C95444343 @default.
- W2007253244 hasIssue "22" @default.
- W2007253244 hasLocation W20072532441 @default.
- W2007253244 hasLocation W20072532442 @default.
- W2007253244 hasLocation W20072532443 @default.
- W2007253244 hasLocation W20072532444 @default.
- W2007253244 hasOpenAccess W2007253244 @default.
- W2007253244 hasPrimaryLocation W20072532441 @default.
- W2007253244 hasRelatedWork W1468280468 @default.
- W2007253244 hasRelatedWork W1830011917 @default.
- W2007253244 hasRelatedWork W1968991344 @default.
- W2007253244 hasRelatedWork W1971756681 @default.
- W2007253244 hasRelatedWork W2058178091 @default.
- W2007253244 hasRelatedWork W2172014355 @default.
- W2007253244 hasRelatedWork W2329913011 @default.
- W2007253244 hasRelatedWork W2800495128 @default.
- W2007253244 hasRelatedWork W2950045261 @default.
- W2007253244 hasRelatedWork W4302558030 @default.
- W2007253244 hasVolume "83" @default.
- W2007253244 isParatext "false" @default.
- W2007253244 isRetracted "false" @default.
- W2007253244 magId "2007253244" @default.
- W2007253244 workType "article" @default.