Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007296746> ?p ?o ?g. }
- W2007296746 endingPage "e1003800" @default.
- W2007296746 startingPage "e1003800" @default.
- W2007296746 abstract "All genomes require a system for avoidance or handling of collisions between the machineries of DNA replication and transcription. We have investigated the roles in this process of the mTERF (mitochondrial transcription termination factor) family members mTTF and mTerf5 in Drosophila melanogaster. The two mTTF binding sites in Drosophila mtDNA, which also bind mTerf5, were found to coincide with major sites of replication pausing. RNAi-mediated knockdown of either factor resulted in mtDNA depletion and developmental arrest. mTTF knockdown decreased site-specific replication pausing, but led to an increase in replication stalling and fork regression in broad zones around each mTTF binding site. Lagging-strand DNA synthesis was impaired, with extended RNA/DNA hybrid segments seen in replication intermediates. This was accompanied by the accumulation of recombination intermediates and nicked/broken mtDNA species. Conversely, mTerf5 knockdown led to enhanced replication pausing at mTTF binding sites, a decrease in fragile replication intermediates containing single-stranded segments, and the disappearance of species containing segments of RNA/DNA hybrid. These findings indicate an essential and previously undescribed role for proteins of the mTERF family in the integration of transcription and DNA replication, preventing unregulated collisions and facilitating productive interactions between the two machineries that are inferred to be essential for completion of lagging-strand DNA synthesis." @default.
- W2007296746 created "2016-06-24" @default.
- W2007296746 creator A5013145284 @default.
- W2007296746 creator A5036199516 @default.
- W2007296746 creator A5046314051 @default.
- W2007296746 creator A5046656497 @default.
- W2007296746 creator A5066630834 @default.
- W2007296746 creator A5067287460 @default.
- W2007296746 creator A5084324370 @default.
- W2007296746 date "2013-09-19" @default.
- W2007296746 modified "2023-09-25" @default.
- W2007296746 title "Mitochondrial Transcription Terminator Family Members mTTF and mTerf5 Have Opposing Roles in Coordination of mtDNA Synthesis" @default.
- W2007296746 cites W140135608 @default.
- W2007296746 cites W145422372 @default.
- W2007296746 cites W1512483303 @default.
- W2007296746 cites W1604458367 @default.
- W2007296746 cites W1957389955 @default.
- W2007296746 cites W1963720723 @default.
- W2007296746 cites W1965306034 @default.
- W2007296746 cites W1967072429 @default.
- W2007296746 cites W1967154926 @default.
- W2007296746 cites W1968197570 @default.
- W2007296746 cites W1975817822 @default.
- W2007296746 cites W1978680674 @default.
- W2007296746 cites W1979276801 @default.
- W2007296746 cites W1979492621 @default.
- W2007296746 cites W1980444804 @default.
- W2007296746 cites W1983594157 @default.
- W2007296746 cites W1984886494 @default.
- W2007296746 cites W1987024381 @default.
- W2007296746 cites W1992036233 @default.
- W2007296746 cites W1993401855 @default.
- W2007296746 cites W1996052541 @default.
- W2007296746 cites W1996109316 @default.
- W2007296746 cites W1996809767 @default.
- W2007296746 cites W2001801970 @default.
- W2007296746 cites W2003239553 @default.
- W2007296746 cites W2003279154 @default.
- W2007296746 cites W2005277952 @default.
- W2007296746 cites W2008043362 @default.
- W2007296746 cites W2008255353 @default.
- W2007296746 cites W2014114000 @default.
- W2007296746 cites W2014254942 @default.
- W2007296746 cites W2019412492 @default.
- W2007296746 cites W2020985206 @default.
- W2007296746 cites W2023475717 @default.
- W2007296746 cites W2023941806 @default.
- W2007296746 cites W2032913442 @default.
- W2007296746 cites W2035257545 @default.
- W2007296746 cites W2039869364 @default.
- W2007296746 cites W2046453744 @default.
- W2007296746 cites W2047225910 @default.
- W2007296746 cites W2053259460 @default.
- W2007296746 cites W2056379548 @default.
- W2007296746 cites W2058244193 @default.
- W2007296746 cites W2059185956 @default.
- W2007296746 cites W2061027931 @default.
- W2007296746 cites W2068464246 @default.
- W2007296746 cites W2071984045 @default.
- W2007296746 cites W2072879515 @default.
- W2007296746 cites W2075195146 @default.
- W2007296746 cites W2077830471 @default.
- W2007296746 cites W2082527361 @default.
- W2007296746 cites W2084927470 @default.
- W2007296746 cites W2088355095 @default.
- W2007296746 cites W2088585248 @default.
- W2007296746 cites W2089556958 @default.
- W2007296746 cites W2090336051 @default.
- W2007296746 cites W2092380274 @default.
- W2007296746 cites W2092413662 @default.
- W2007296746 cites W2092910502 @default.
- W2007296746 cites W2095861466 @default.
- W2007296746 cites W2107177579 @default.
- W2007296746 cites W2109989513 @default.
- W2007296746 cites W2111978748 @default.
- W2007296746 cites W2113062596 @default.
- W2007296746 cites W2113942479 @default.
- W2007296746 cites W2117031151 @default.
- W2007296746 cites W2119276005 @default.
- W2007296746 cites W2120745370 @default.
- W2007296746 cites W2124982778 @default.
- W2007296746 cites W2126354256 @default.
- W2007296746 cites W2138676769 @default.
- W2007296746 cites W2144567287 @default.
- W2007296746 cites W2146482482 @default.
- W2007296746 cites W2149947954 @default.
- W2007296746 cites W2155949689 @default.
- W2007296746 cites W2158737337 @default.
- W2007296746 cites W2160336344 @default.
- W2007296746 cites W2161116308 @default.
- W2007296746 cites W2162928159 @default.
- W2007296746 cites W2163733636 @default.
- W2007296746 cites W2165988320 @default.
- W2007296746 cites W2171469487 @default.
- W2007296746 cites W2171821318 @default.
- W2007296746 cites W2330644852 @default.
- W2007296746 cites W293285070 @default.
- W2007296746 cites W4233901565 @default.