FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007298093> ?p ?o ?g. }

• W2007298093 endingPage "48" @default.
• W2007298093 startingPage "39" @default.
• W2007298093 abstract "Results of studies from our laboratory have shown that administration of 17β-estradiol (E2) reduces cerebellar neuronal damage during ethanol withdrawal (EW). In the current study, we examined mechanisms underlying E2 protection against EW-associated cerebellar damage by assessing apoptotic indicators: DNA fragmentation, caspase-3 activity, and protein kinase C (PKC) activity. Ovariectomized rats, implanted with E2 or oil pellets, received ethanol [7.5% weight/volume (wt./vol.)] (EW/E2 group and EW/Oil group, respectively) chronically (for 5 weeks) or control dextrin diet (Dextrin/Oil group). At day 14 of EW, cerebelli were collected for the terminal deoxynucleotidyltransferase (TdT)-mediated dUDP-biotin nick end labeling (TUNEL) assay to detect DNA fragmentation and for immunohistochemistry to detect caspase-3 activation. A separate group of rat cerebelli was prepared to assess for total PKC activity, as well as for activity of a specific PKC isozyme, epsilon (PKCε), by using an in vitro [γ-32P]ATP phosphorylation assay at days 1 and 14 of EW. Results indicated that rats in the EW/Oil group had more DNA fragments and caspase-3–positive neuronal cells than observed for control rats, and these effects were inhibited by E2 treatment. For total PKC activity at day 1 of EW, rats in the EW/E2 group had a lower cytosolic PKC activity than observed for either rats in the EW/Oil group or control rats. At day 14 of EW, both EW groups had a lower total PKC activity than observed for control rats. For PKCε activity, rats in the EW/E2 group had a lower cytosolic PKCε activity than observed for rats in the EW/Oil group or for control rats at day 1, and they had a lower membrane PKCε activity at day 14 of EW than observed for control rats. These findings support the suggestion that E2 protects against cerebellar neuronal damage in ethanol-withdrawn rats by inhibition of DNA fragmentation and caspase-3 activation, and that reduced PKC activity may be involved in the protection." @default.
• W2007298093 created "2016-06-24" @default.
• W2007298093 creator A5024146315 @default.
• W2007298093 creator A5024835172 @default.
• W2007298093 creator A5035736015 @default.
• W2007298093 creator A5069174970 @default.
• W2007298093 date "2003-08-01" @default.
• W2007298093 modified "2023-10-15" @default.
• W2007298093 title "Role of protein kinase C in estrogen protection against apoptotic cerebellar cell death in ethanol-withdrawn rats" @default.
• W2007298093 cites W1486333767 @default.
• W2007298093 cites W1506768719 @default.
• W2007298093 cites W1517614087 @default.
• W2007298093 cites W1701778517 @default.
• W2007298093 cites W1786056430 @default.
• W2007298093 cites W1837586904 @default.
• W2007298093 cites W1838634581 @default.
• W2007298093 cites W1975363851 @default.
• W2007298093 cites W1978991174 @default.
• W2007298093 cites W1981939894 @default.
• W2007298093 cites W1991972636 @default.
• W2007298093 cites W1992331688 @default.
• W2007298093 cites W1992889151 @default.
• W2007298093 cites W1992958781 @default.
• W2007298093 cites W1995538168 @default.
• W2007298093 cites W1998405135 @default.
• W2007298093 cites W1999987807 @default.
• W2007298093 cites W2002879969 @default.
• W2007298093 cites W2008186920 @default.
• W2007298093 cites W2008258524 @default.
• W2007298093 cites W2009060563 @default.
• W2007298093 cites W2014040726 @default.
• W2007298093 cites W2014102011 @default.
• W2007298093 cites W2016717357 @default.
• W2007298093 cites W2021407509 @default.
• W2007298093 cites W2021677873 @default.
• W2007298093 cites W2022437462 @default.
• W2007298093 cites W2024081559 @default.
• W2007298093 cites W2032144305 @default.
• W2007298093 cites W2032755712 @default.
• W2007298093 cites W2036869755 @default.
• W2007298093 cites W2042668042 @default.
• W2007298093 cites W2043348076 @default.
• W2007298093 cites W2045718847 @default.
• W2007298093 cites W2047281776 @default.
• W2007298093 cites W2048684469 @default.
• W2007298093 cites W2053261189 @default.
• W2007298093 cites W2058326411 @default.
• W2007298093 cites W2060488660 @default.
• W2007298093 cites W2061497413 @default.
• W2007298093 cites W2062904343 @default.
• W2007298093 cites W2063225451 @default.
• W2007298093 cites W2067899258 @default.
• W2007298093 cites W2070035008 @default.
• W2007298093 cites W2072286244 @default.
• W2007298093 cites W2074823342 @default.
• W2007298093 cites W2077513367 @default.
• W2007298093 cites W2078877036 @default.
• W2007298093 cites W2082433420 @default.
• W2007298093 cites W2082648762 @default.
• W2007298093 cites W2088472722 @default.
• W2007298093 cites W2089475281 @default.
• W2007298093 cites W2095362651 @default.
• W2007298093 cites W2121784259 @default.
• W2007298093 cites W2122680359 @default.
• W2007298093 cites W2134320754 @default.
• W2007298093 cites W2142541950 @default.
• W2007298093 cites W2153990155 @default.
• W2007298093 cites W2154964438 @default.
• W2007298093 cites W2159984609 @default.
• W2007298093 cites W2162665706 @default.
• W2007298093 cites W2178730998 @default.
• W2007298093 cites W2316991189 @default.
• W2007298093 cites W4236030394 @default.
• W2007298093 cites W4241023416 @default.
• W2007298093 cites W4313347840 @default.
• W2007298093 doi "https://doi.org/10.1016/j.alcohol.2003.07.004" @default.
• W2007298093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14615010" @default.
• W2007298093 hasPublicationYear "2003" @default.
• W2007298093 type Work @default.
• W2007298093 sameAs 2007298093 @default.
• W2007298093 citedByCount "16" @default.
• W2007298093 countsByYear W20072980932018 @default.
• W2007298093 crossrefType "journal-article" @default.
• W2007298093 hasAuthorship W2007298093A5024146315 @default.
• W2007298093 hasAuthorship W2007298093A5024835172 @default.
• W2007298093 hasAuthorship W2007298093A5035736015 @default.
• W2007298093 hasAuthorship W2007298093A5069174970 @default.
• W2007298093 hasConcept C11960822 @default.
• W2007298093 hasConcept C126322002 @default.
• W2007298093 hasConcept C134018914 @default.
• W2007298093 hasConcept C153911025 @default.
• W2007298093 hasConcept C16685009 @default.
• W2007298093 hasConcept C185592680 @default.
• W2007298093 hasConcept C190283241 @default.
• W2007298093 hasConcept C195794163 @default.
• W2007298093 hasConcept C196795494 @default.
• W2007298093 hasConcept C2777164284 @default.
• W2007298093 hasConcept C31573885 @default.

• next