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• W2007304295 abstract "Objectives: Exogenously added Reactive Oxygen Species (ROS) have been known to be detrimental or beneficial to mammalian sperm function. The higher levels of ROS, especially Hydrogen peroxide (H2O2), produced by damaged spermatozoa were associated with a loss of motility and decreased capacity for sperm-oocyte fusion. The objective of this study was to investigate whether the low levels of H2O2 could be detrimental to sperm motility and fertilization ability. Design: ICR mouse spermatozoa were preincubated with or without H2O2 for 30 min before in vitro fertilization (IVF), and sperm motility, fertilization rate were determined. Materials and Methods: The first study examined the effects of three concentrations of H2O2 in 30 min. incubation of mouse spermatozoa, prior to IVF. ICR mouse spermatozoa released from the cauda epididymis were exposed to H2O2 (0.05 mM, 0.1 mM and 0.2 mM) at 37°C in BMOC-3 medium for 30 min, washed on a mini-Percoll gradient and were divided into two groups. One was immediately used for insemination (with oocyte from ICR mice, aged 7–8 weeks) at a final concentration of 106 motile cell/ml. The other was used for evaluation of the H2O2 generation, which was assessed by the horseradish peroxide (HROP)-dependent oxidation. Results: Sperm motility were significantly decreased when exposed to 0.1 mM and 0.2 mM of H2O2 compared with non-H2O2 exposed spermatozoa (73.5 ± 7.3% vs. 62.4 ± 7.8%, 59.0 ± 14.5%, p<0.05 respectively). No such decrease was observed in spermatozoa exposed to 0.05 mM of H2O2 (72.8 ± 11.6%). The fertilization rates of spermatozoa exposed to 0.01mM and 0.02mM of H2O2 were significantly lower than that observed with non-H2O2 exposed spermatozoa (55.2% vs. 17.6%, 11.3%, p<0.05 respectively), and 0.05 mM of H2O2 exposed spermatozoa (55.2% vs. 36.8%, p<0.05). The generation of H2O2 when various concentrations of H2O2 (0.05 mM, 0.1 mM and 0.2 mM) were added to the sperm suspension, were increased depend on the concentration compared for non-H2O2 exposed sperm suspension (1.5, 2.1, 2.9-fold, respectively). Conclusions: These results suggest that H2O2 is the toxic ROS responsible for motility of mouse spermatozoa and the capacity of fertilization to spermatozoa. However, H2O2 may influence the capacity of fertilization to spermatozoa even with short time exposure to very low concentrations of H2O2 without affecting sperm motility." @default.
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• W2007304295 date "2000-09-01" @default.
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• W2007304295 title "Hydrogen Peroxide-Induced Oxidative Stress Impairs Motility and Fertilization Rate In Vitro of Mouse Spermatozoa" @default.
• W2007304295 doi "https://doi.org/10.1016/s0015-0282(00)01442-4" @default.
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