FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007316441> ?p ?o ?g. }

• W2007316441 endingPage "6752" @default.
• W2007316441 startingPage "6743" @default.
• W2007316441 abstract "Among the factors that modulate protein interactions, several protein structural properties, such as size, shape, and charge distribution, may play significant roles. In this work, we investigate the influence of protein structure on binding in ion-exchange chromatography, in which electrostatic interactions are dominant. Chromatographic experiments show separation of cytochrome c variants with a limited number of sequence differences to be feasible. To probe the molecular basis for this behavior, protein−adsorbent electrostatic interactions were modeled in the context of continuum electrostatics accounting for the full 3D protein structure. Protein retention was modeled by averaging over all protein−adsorbent configurations using the full accessible surface of the protein. The electrostatic interaction free energy distribution shows that configurations in which numerous positive protein charges are close to the cation exchanger functional groups produce the most favorable binding. The calculated binding equilibrium constant, found by averaging over the full 3D configurational space, captures the chromatographic differentiation of closely related cytochrome c variants. To obviate the need for full sampling of protein configurations, calculations of interaction free energies at short protein−adsorbent separation distances or of protein surface potentials were found to yield reasonable semiquantitative descriptions of the retention trends." @default.
• W2007316441 created "2016-06-24" @default.
• W2007316441 creator A5018451658 @default.
• W2007316441 creator A5089483150 @default.
• W2007316441 date "2004-10-12" @default.
• W2007316441 modified "2023-09-23" @default.
• W2007316441 title "Electrostatic Contributions to Protein Retention in Ion-Exchange Chromatography. 1. Cytochrome <i>c</i> Variants" @default.
• W2007316441 cites W1604894604 @default.
• W2007316441 cites W1894569347 @default.
• W2007316441 cites W1965380027 @default.
• W2007316441 cites W1966041739 @default.
• W2007316441 cites W1972620015 @default.
• W2007316441 cites W1981925074 @default.
• W2007316441 cites W1989876752 @default.
• W2007316441 cites W1992318276 @default.
• W2007316441 cites W2000033940 @default.
• W2007316441 cites W2009792672 @default.
• W2007316441 cites W2023504751 @default.
• W2007316441 cites W2029667189 @default.
• W2007316441 cites W2032518125 @default.
• W2007316441 cites W2039302123 @default.
• W2007316441 cites W2051568142 @default.
• W2007316441 cites W2056180063 @default.
• W2007316441 cites W2063820532 @default.
• W2007316441 cites W2066142272 @default.
• W2007316441 cites W2069616361 @default.
• W2007316441 cites W2071657062 @default.
• W2007316441 cites W2075415646 @default.
• W2007316441 cites W2078269273 @default.
• W2007316441 cites W2079243965 @default.
• W2007316441 cites W2081198406 @default.
• W2007316441 cites W2081588627 @default.
• W2007316441 cites W2088316911 @default.
• W2007316441 cites W2090233594 @default.
• W2007316441 cites W2234332767 @default.
• W2007316441 cites W53817057 @default.
• W2007316441 doi "https://doi.org/10.1021/ac049327z" @default.
• W2007316441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15538799" @default.
• W2007316441 hasPublicationYear "2004" @default.
• W2007316441 type Work @default.
• W2007316441 sameAs 2007316441 @default.
• W2007316441 citedByCount "42" @default.
• W2007316441 countsByYear W20073164412012 @default.
• W2007316441 countsByYear W20073164412013 @default.
• W2007316441 countsByYear W20073164412014 @default.
• W2007316441 countsByYear W20073164412015 @default.
• W2007316441 countsByYear W20073164412016 @default.
• W2007316441 countsByYear W20073164412017 @default.
• W2007316441 countsByYear W20073164412018 @default.
• W2007316441 countsByYear W20073164412019 @default.
• W2007316441 countsByYear W20073164412020 @default.
• W2007316441 countsByYear W20073164412021 @default.
• W2007316441 countsByYear W20073164412023 @default.
• W2007316441 crossrefType "journal-article" @default.
• W2007316441 hasAuthorship W2007316441A5018451658 @default.
• W2007316441 hasAuthorship W2007316441A5089483150 @default.
• W2007316441 hasConcept C10710636 @default.
• W2007316441 hasConcept C113196181 @default.
• W2007316441 hasConcept C117626034 @default.
• W2007316441 hasConcept C119599485 @default.
• W2007316441 hasConcept C127413603 @default.
• W2007316441 hasConcept C147789679 @default.
• W2007316441 hasConcept C150394285 @default.
• W2007316441 hasConcept C151730666 @default.
• W2007316441 hasConcept C159467904 @default.
• W2007316441 hasConcept C185592680 @default.
• W2007316441 hasConcept C2779343474 @default.
• W2007316441 hasConcept C28859421 @default.
• W2007316441 hasConcept C29311851 @default.
• W2007316441 hasConcept C43617362 @default.
• W2007316441 hasConcept C55493867 @default.
• W2007316441 hasConcept C86803240 @default.
• W2007316441 hasConcept C87716614 @default.
• W2007316441 hasConcept C88001094 @default.
• W2007316441 hasConceptScore W2007316441C10710636 @default.
• W2007316441 hasConceptScore W2007316441C113196181 @default.
• W2007316441 hasConceptScore W2007316441C117626034 @default.
• W2007316441 hasConceptScore W2007316441C119599485 @default.
• W2007316441 hasConceptScore W2007316441C127413603 @default.
• W2007316441 hasConceptScore W2007316441C147789679 @default.
• W2007316441 hasConceptScore W2007316441C150394285 @default.
• W2007316441 hasConceptScore W2007316441C151730666 @default.
• W2007316441 hasConceptScore W2007316441C159467904 @default.
• W2007316441 hasConceptScore W2007316441C185592680 @default.
• W2007316441 hasConceptScore W2007316441C2779343474 @default.
• W2007316441 hasConceptScore W2007316441C28859421 @default.
• W2007316441 hasConceptScore W2007316441C29311851 @default.
• W2007316441 hasConceptScore W2007316441C43617362 @default.
• W2007316441 hasConceptScore W2007316441C55493867 @default.
• W2007316441 hasConceptScore W2007316441C86803240 @default.
• W2007316441 hasConceptScore W2007316441C87716614 @default.
• W2007316441 hasConceptScore W2007316441C88001094 @default.
• W2007316441 hasIssue "22" @default.
• W2007316441 hasLocation W20073164411 @default.
• W2007316441 hasLocation W20073164412 @default.
• W2007316441 hasOpenAccess W2007316441 @default.
• W2007316441 hasPrimaryLocation W20073164411 @default.
• W2007316441 hasRelatedWork W1595274355 @default.

• next