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- W2007352024 abstract "<h3>Background and Objectives.</h3> 2-Chloroprocaine is rapidly metabolized in the blood to yield 2-chloro-para-aminobenzoic acid (an inactive metabolite) and diethylaminoethanol (DEAE). DEAE possesses local anesthetic activity. The only reported assay for DEAE is a colorimetric method. <h3>Methods.</h3> Clinical samples of whole blood and serum were obtained from patients receiving stepped intravenous infusions of 3% 2-chloroprocaine. A high pH-dependent liquid-liquid extraction step with diethyl ether was used to eliminate interfering peaks in high-pressure liquid chromatography (HPLC) analysis. Separation and quantitation were performed using HPLC on a polymeric-reversed phase column with a mobile phase consisting of 10% or 20% acetonitrile (for whole blood or serum analysis, respectively) in 50 mm aqueous sodium phosphate buffer, pH = 11.50. The elution order of DEAE and its analogues was tested to interpret the HPLC separation mechanism. <h3>Results.</h3> Extraction recovery of DEAE from whole blood was 67 ± 13.5%, from serum, 71 ± 12.2%, and from water, 75 ± 2.9%. The high pH value of the mobile phase resulted in sharp, well-resolved peaks with run times of approximately 8 minutes using 20% acetonitrile. The lower limit of detection was 5 ng/mL of DEAE from a 1-mL sample. The elution order of DEAE and its analogues indicated that separation was based on the hydrophobicity of the analytes rather than polar group interactions occurring with silica-based stationary phase. <h3>Conclusions.</h3> A new, simple and rapid HPLC method for extraction and measurement of DEAE in whole blood or serum samples is reported here." @default.
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- W2007352024 date "1999-12-04" @default.
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- W2007352024 title "Improving NHS performance: human behaviour and health policy" @default.
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- W2007352024 doi "https://doi.org/10.1136/bmj.319.7223.1490" @default.
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