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- W2007365741 abstract "A correlation has been sought between the effects of lipids on membrane fluidity and mitogen responsiveness of human peripheral blood lymphocytes (PBL). Cholesterol and the reputedly potent membrane fluidizing agent AL721 (1) were used for these studies. However, the large AL721 induced increase in membrane fluidity, assessed by steady state polarization of the probe 1,6-diphenyl-1,3,5-hexatriene (DPH), was found to be an artefact arising from lipid uptake by monocytes. Mitogen responses were enhanced by AL721 but unaffected by cholesterol. It is concluded that AL721 does not exert its effect through enhanced triggering of cells by altered membrane fluidity but rather that lymphocytes require an exogenous source of phospholipids/triglycerides for optimal growth in vitro, although they can synthesize sufficient cholesterol to meet their own needs." @default.
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- W2007365741 date "1988-03-01" @default.
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- W2007365741 title "Lipid requirements of human t lymphocytes stimulated with mitogen in serum-free medium. Membrane «fluidity» changes are an artefact of lipid (AL721)uptake by monocytes" @default.
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- W2007365741 doi "https://doi.org/10.1016/s0171-2985(88)80026-3" @default.
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