Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007384310> ?p ?o ?g. }
- W2007384310 endingPage "5728" @default.
- W2007384310 startingPage "5720" @default.
- W2007384310 abstract "DNA microarray analysis has been applied to identify molecular markers of human hematological malignancies. However, the relatively low correlation between the abundance of a given mRNA and that of the encoded protein makes it important to characterize the protein profile directly, or ‘proteome,’ of malignant cells in addition to the ‘transcriptome.’ To identify proteins specifically expressed in leukemias, here we isolated AC133+ hematopoietic stem cell-like fractions from the bone marrow of 13 individuals with various leukemic disorders, and compared their protein profiles by two-dimensional electrophoresis. A total of 11 differentially expressed protein spots corresponding to 10 independent proteins were detected, and peptide fingerprinting combined with mass spectrometry of these proteins revealed them to include NuMA (nuclear protein that associates with the mitotic apparatus), heat shock proteins, and redox regulators. The abundance of NuMA in the leukemic blasts was significantly related to the presence of complex karyotype anomalies. Conditional expression of NuMA in a mouse myeloid cell line resulted in the induction of aneuploidy, cell cycle arrest in G2–M phases, and apoptosis. These results demonstrate the potential of proteome analysis with background-matched cell fractions obtained from fresh clinical specimens to provide insight into the mechanism of human leukemogenesis." @default.
- W2007384310 created "2016-06-24" @default.
- W2007384310 creator A5001514470 @default.
- W2007384310 creator A5013310458 @default.
- W2007384310 creator A5013574242 @default.
- W2007384310 creator A5018621864 @default.
- W2007384310 creator A5041829158 @default.
- W2007384310 creator A5049785426 @default.
- W2007384310 creator A5054148551 @default.
- W2007384310 creator A5059055930 @default.
- W2007384310 creator A5066060747 @default.
- W2007384310 creator A5068637876 @default.
- W2007384310 creator A5074799003 @default.
- W2007384310 creator A5076014498 @default.
- W2007384310 creator A5089756558 @default.
- W2007384310 creator A5090560513 @default.
- W2007384310 date "2003-08-28" @default.
- W2007384310 modified "2023-09-26" @default.
- W2007384310 title "Proteomic analysis of hematopoietic stem cell-like fractions in leukemic disorders" @default.
- W2007384310 cites W1982959896 @default.
- W2007384310 cites W1987514898 @default.
- W2007384310 cites W2013758383 @default.
- W2007384310 cites W2045621075 @default.
- W2007384310 cites W2046507097 @default.
- W2007384310 cites W2048659987 @default.
- W2007384310 cites W2051099851 @default.
- W2007384310 cites W2054015931 @default.
- W2007384310 cites W2065406588 @default.
- W2007384310 cites W2068513215 @default.
- W2007384310 cites W2078594804 @default.
- W2007384310 cites W2080552851 @default.
- W2007384310 cites W2094613397 @default.
- W2007384310 cites W2094782116 @default.
- W2007384310 cites W2105969460 @default.
- W2007384310 cites W2109363337 @default.
- W2007384310 cites W2119471586 @default.
- W2007384310 cites W2127230663 @default.
- W2007384310 cites W2147246240 @default.
- W2007384310 cites W2150081412 @default.
- W2007384310 cites W2153719235 @default.
- W2007384310 cites W2162859804 @default.
- W2007384310 cites W2170949754 @default.
- W2007384310 cites W2330120499 @default.
- W2007384310 cites W4246697467 @default.
- W2007384310 doi "https://doi.org/10.1038/sj.onc.1206855" @default.
- W2007384310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12944923" @default.
- W2007384310 hasPublicationYear "2003" @default.
- W2007384310 type Work @default.
- W2007384310 sameAs 2007384310 @default.
- W2007384310 citedByCount "49" @default.
- W2007384310 countsByYear W20073843102012 @default.
- W2007384310 countsByYear W20073843102014 @default.
- W2007384310 countsByYear W20073843102015 @default.
- W2007384310 countsByYear W20073843102017 @default.
- W2007384310 countsByYear W20073843102018 @default.
- W2007384310 countsByYear W20073843102022 @default.
- W2007384310 countsByYear W20073843102023 @default.
- W2007384310 crossrefType "journal-article" @default.
- W2007384310 hasAuthorship W2007384310A5001514470 @default.
- W2007384310 hasAuthorship W2007384310A5013310458 @default.
- W2007384310 hasAuthorship W2007384310A5013574242 @default.
- W2007384310 hasAuthorship W2007384310A5018621864 @default.
- W2007384310 hasAuthorship W2007384310A5041829158 @default.
- W2007384310 hasAuthorship W2007384310A5049785426 @default.
- W2007384310 hasAuthorship W2007384310A5054148551 @default.
- W2007384310 hasAuthorship W2007384310A5059055930 @default.
- W2007384310 hasAuthorship W2007384310A5066060747 @default.
- W2007384310 hasAuthorship W2007384310A5068637876 @default.
- W2007384310 hasAuthorship W2007384310A5074799003 @default.
- W2007384310 hasAuthorship W2007384310A5076014498 @default.
- W2007384310 hasAuthorship W2007384310A5089756558 @default.
- W2007384310 hasAuthorship W2007384310A5090560513 @default.
- W2007384310 hasBestOaLocation W20073843101 @default.
- W2007384310 hasConcept C104317684 @default.
- W2007384310 hasConcept C104397665 @default.
- W2007384310 hasConcept C109159458 @default.
- W2007384310 hasConcept C1491633281 @default.
- W2007384310 hasConcept C150194340 @default.
- W2007384310 hasConcept C153911025 @default.
- W2007384310 hasConcept C162317418 @default.
- W2007384310 hasConcept C201750760 @default.
- W2007384310 hasConcept C205260736 @default.
- W2007384310 hasConcept C2779282312 @default.
- W2007384310 hasConcept C28328180 @default.
- W2007384310 hasConcept C29537977 @default.
- W2007384310 hasConcept C46111723 @default.
- W2007384310 hasConcept C502942594 @default.
- W2007384310 hasConcept C54355233 @default.
- W2007384310 hasConcept C86803240 @default.
- W2007384310 hasConcept C93304396 @default.
- W2007384310 hasConcept C95444343 @default.
- W2007384310 hasConceptScore W2007384310C104317684 @default.
- W2007384310 hasConceptScore W2007384310C104397665 @default.
- W2007384310 hasConceptScore W2007384310C109159458 @default.
- W2007384310 hasConceptScore W2007384310C1491633281 @default.
- W2007384310 hasConceptScore W2007384310C150194340 @default.
- W2007384310 hasConceptScore W2007384310C153911025 @default.
- W2007384310 hasConceptScore W2007384310C162317418 @default.