FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007394420> ?p ?o ?g. }

• W2007394420 endingPage "418" @default.
• W2007394420 startingPage "407" @default.
• W2007394420 abstract "Overexpression of the hexose/proton symporter HUP1 from Chlorella kessleri in S. cerevisiae permits a one-step purification via a biotinylation domain. Milligram amounts of the protein are obtained starting from 2 l of yeast culture. The HUP1 protein is used as a model eukaryotic membrane protein of the 'major facilitator superfamily' (MFS) to study specific lipid requirements for activity and stability. Testing two series of detergents revealed that n-nonyl-beta-D-glucoside (NG) and n-octyl-beta-D-glucoside (OG) solubilize the HUP1 protein efficiently. Only the use of NG resulted in long-term stabilization of the HUP1 protein in the absence of external lipids. When affinity purified protein was extracted with organic solvents, a stoichiometric amount of phosphatidyl choline, phosphatidyl ethanolamine and ergosterol in the ratio of close to 2:1 was detected. These lipids were only observed, however, when the protein purification was carried out in the presence of NG; no lipids were copurified with the HUP1 protein in the presence of OG. Of the three lipids copurified, phosphatidyl choline showed a crucial role in ensuring maximal HUP1 permease activity and stability when added back to the OG-protein. The requirement of phosphatidylcholine documents a specific effect of lipids on vectorial transport mediated by a eukaryotic membrane protein of the MFS family." @default.
• W2007394420 created "2016-06-24" @default.
• W2007394420 creator A5048369044 @default.
• W2007394420 creator A5052559958 @default.
• W2007394420 creator A5062778363 @default.
• W2007394420 creator A5077810277 @default.
• W2007394420 date "2000-02-01" @default.
• W2007394420 modified "2023-10-17" @default.
• W2007394420 title "Properties of a reconstituted eukaryotic hexose/proton symporter solubilized by structurally related non-ionic detergents: specific requirement of phosphatidylcholine for permease stability" @default.
• W2007394420 cites W1044851296 @default.
• W2007394420 cites W1493642813 @default.
• W2007394420 cites W1545584842 @default.
• W2007394420 cites W175843418 @default.
• W2007394420 cites W1792853116 @default.
• W2007394420 cites W1927096570 @default.
• W2007394420 cites W1964929902 @default.
• W2007394420 cites W1967888351 @default.
• W2007394420 cites W1968454024 @default.
• W2007394420 cites W1986015316 @default.
• W2007394420 cites W1991758707 @default.
• W2007394420 cites W1995695956 @default.
• W2007394420 cites W2017423684 @default.
• W2007394420 cites W2017760337 @default.
• W2007394420 cites W2030243917 @default.
• W2007394420 cites W2036788087 @default.
• W2007394420 cites W2050826023 @default.
• W2007394420 cites W2053315485 @default.
• W2007394420 cites W2057241898 @default.
• W2007394420 cites W2059285304 @default.
• W2007394420 cites W2067353791 @default.
• W2007394420 cites W2088263411 @default.
• W2007394420 cites W2089388579 @default.
• W2007394420 cites W2113442449 @default.
• W2007394420 cites W2130381741 @default.
• W2007394420 cites W2139399311 @default.
• W2007394420 cites W2145185052 @default.
• W2007394420 cites W2150264392 @default.
• W2007394420 cites W2161773538 @default.
• W2007394420 cites W2171101358 @default.
• W2007394420 cites W2394956077 @default.
• W2007394420 doi "https://doi.org/10.1016/s0005-2736(99)00229-1" @default.
• W2007394420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10675517" @default.
• W2007394420 hasPublicationYear "2000" @default.
• W2007394420 type Work @default.
• W2007394420 sameAs 2007394420 @default.
• W2007394420 citedByCount "17" @default.
• W2007394420 countsByYear W20073944202013 @default.
• W2007394420 countsByYear W20073944202014 @default.
• W2007394420 countsByYear W20073944202021 @default.
• W2007394420 crossrefType "journal-article" @default.
• W2007394420 hasAuthorship W2007394420A5048369044 @default.
• W2007394420 hasAuthorship W2007394420A5052559958 @default.
• W2007394420 hasAuthorship W2007394420A5062778363 @default.
• W2007394420 hasAuthorship W2007394420A5077810277 @default.
• W2007394420 hasBestOaLocation W20073944201 @default.
• W2007394420 hasConcept C104317684 @default.
• W2007394420 hasConcept C120405084 @default.
• W2007394420 hasConcept C149011108 @default.
• W2007394420 hasConcept C181199279 @default.
• W2007394420 hasConcept C185592680 @default.
• W2007394420 hasConcept C190114821 @default.
• W2007394420 hasConcept C204628709 @default.
• W2007394420 hasConcept C2776330855 @default.
• W2007394420 hasConcept C2776393687 @default.
• W2007394420 hasConcept C2778918659 @default.
• W2007394420 hasConcept C2778987810 @default.
• W2007394420 hasConcept C41625074 @default.
• W2007394420 hasConcept C43617362 @default.
• W2007394420 hasConcept C55493867 @default.
• W2007394420 hasConcept C62465871 @default.
• W2007394420 hasConceptScore W2007394420C104317684 @default.
• W2007394420 hasConceptScore W2007394420C120405084 @default.
• W2007394420 hasConceptScore W2007394420C149011108 @default.
• W2007394420 hasConceptScore W2007394420C181199279 @default.
• W2007394420 hasConceptScore W2007394420C185592680 @default.
• W2007394420 hasConceptScore W2007394420C190114821 @default.
• W2007394420 hasConceptScore W2007394420C204628709 @default.
• W2007394420 hasConceptScore W2007394420C2776330855 @default.
• W2007394420 hasConceptScore W2007394420C2776393687 @default.
• W2007394420 hasConceptScore W2007394420C2778918659 @default.
• W2007394420 hasConceptScore W2007394420C2778987810 @default.
• W2007394420 hasConceptScore W2007394420C41625074 @default.
• W2007394420 hasConceptScore W2007394420C43617362 @default.
• W2007394420 hasConceptScore W2007394420C55493867 @default.
• W2007394420 hasConceptScore W2007394420C62465871 @default.
• W2007394420 hasIssue "2" @default.
• W2007394420 hasLocation W20073944201 @default.
• W2007394420 hasLocation W20073944202 @default.
• W2007394420 hasOpenAccess W2007394420 @default.
• W2007394420 hasPrimaryLocation W20073944201 @default.
• W2007394420 hasRelatedWork W2007394420 @default.
• W2007394420 hasRelatedWork W2013616600 @default.
• W2007394420 hasRelatedWork W2033976445 @default.
• W2007394420 hasRelatedWork W2097877929 @default.
• W2007394420 hasRelatedWork W2110222269 @default.
• W2007394420 hasRelatedWork W2110392111 @default.
• W2007394420 hasRelatedWork W2111345489 @default.
• W2007394420 hasRelatedWork W2170420869 @default.

• next