FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007402269> ?p ?o ?g. }

• W2007402269 endingPage "409" @default.
• W2007402269 startingPage "397" @default.
• W2007402269 abstract "Eukaryotic genomes encode a considerably higher fraction of multi-domain proteins than their prokaryotic counterparts. It has been postulated that efficient co-translational and sequential domain folding has facilitated the explosive evolution of multi-domain proteins in eukaryotes by the recombination of pre-existent domains. Here, we tested whether eukaryotes and bacteria differ generally in the folding efficiency of multi-domain proteins generated by domain recombination. To this end, we compared the folding behavior of a series of recombinant proteins comprised of green fluorescent protein (GFP) fused to four different robustly folding proteins through six different linkers upon expression in Escherichia coli and the yeast Saccharomyces cerevisiae. We found that, unlike yeast, bacteria are remarkably inefficient at folding these fusion proteins, even at comparable levels of expression. In vitro and in vivo folding experiments demonstrate that the GFP domain imposes significant constraints on de novo folding of its fusion partners in bacteria, consistent with a largely post-translational folding mechanism. This behavior may result from an interference of GFP with adjacent domains during folding due to the particular topology of the β-barrel GFP structure. By following the accumulation of enzymatic activity, we found that the rate of appearance of correctly folded fusion protein per ribosome is indeed considerably higher in yeast than in bacteria." @default.
• W2007402269 created "2016-06-24" @default.
• W2007402269 creator A5038932122 @default.
• W2007402269 creator A5053848388 @default.
• W2007402269 creator A5058920778 @default.
• W2007402269 creator A5076779327 @default.
• W2007402269 date "2005-10-01" @default.
• W2007402269 modified "2023-10-16" @default.
• W2007402269 title "De novo Folding of GFP Fusion Proteins: High Efficiency in Eukaryotes but Not in Bacteria" @default.
• W2007402269 cites W1519297291 @default.
• W2007402269 cites W1795716959 @default.
• W2007402269 cites W1854687733 @default.
• W2007402269 cites W1968218728 @default.
• W2007402269 cites W1970756887 @default.
• W2007402269 cites W1974440020 @default.
• W2007402269 cites W1983093761 @default.
• W2007402269 cites W1987209219 @default.
• W2007402269 cites W1992256434 @default.
• W2007402269 cites W2000456081 @default.
• W2007402269 cites W2005965349 @default.
• W2007402269 cites W2017526934 @default.
• W2007402269 cites W2023096047 @default.
• W2007402269 cites W2028014512 @default.
• W2007402269 cites W2030695915 @default.
• W2007402269 cites W2036742051 @default.
• W2007402269 cites W2041914510 @default.
• W2007402269 cites W2049883153 @default.
• W2007402269 cites W2056333944 @default.
• W2007402269 cites W2057172252 @default.
• W2007402269 cites W2069496952 @default.
• W2007402269 cites W2073785345 @default.
• W2007402269 cites W2080071375 @default.
• W2007402269 cites W2087823077 @default.
• W2007402269 cites W2088773283 @default.
• W2007402269 cites W2089597488 @default.
• W2007402269 cites W2096966529 @default.
• W2007402269 cites W2105422109 @default.
• W2007402269 cites W2114522205 @default.
• W2007402269 cites W2127068865 @default.
• W2007402269 cites W2128000228 @default.
• W2007402269 cites W2130579232 @default.
• W2007402269 cites W2130581715 @default.
• W2007402269 cites W2137450588 @default.
• W2007402269 cites W2137663279 @default.
• W2007402269 cites W2145038487 @default.
• W2007402269 cites W2147289825 @default.
• W2007402269 cites W2149198894 @default.
• W2007402269 cites W2160084442 @default.
• W2007402269 cites W2170471085 @default.
• W2007402269 cites W4251838592 @default.
• W2007402269 doi "https://doi.org/10.1016/j.jmb.2005.08.052" @default.
• W2007402269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16171814" @default.
• W2007402269 hasPublicationYear "2005" @default.
• W2007402269 type Work @default.
• W2007402269 sameAs 2007402269 @default.
• W2007402269 citedByCount "95" @default.
• W2007402269 countsByYear W20074022692012 @default.
• W2007402269 countsByYear W20074022692013 @default.
• W2007402269 countsByYear W20074022692014 @default.
• W2007402269 countsByYear W20074022692015 @default.
• W2007402269 countsByYear W20074022692016 @default.
• W2007402269 countsByYear W20074022692017 @default.
• W2007402269 countsByYear W20074022692018 @default.
• W2007402269 countsByYear W20074022692019 @default.
• W2007402269 countsByYear W20074022692020 @default.
• W2007402269 countsByYear W20074022692021 @default.
• W2007402269 countsByYear W20074022692022 @default.
• W2007402269 countsByYear W20074022692023 @default.
• W2007402269 crossrefType "journal-article" @default.
• W2007402269 hasAuthorship W2007402269A5038932122 @default.
• W2007402269 hasAuthorship W2007402269A5053848388 @default.
• W2007402269 hasAuthorship W2007402269A5058920778 @default.
• W2007402269 hasAuthorship W2007402269A5076779327 @default.
• W2007402269 hasConcept C104317684 @default.
• W2007402269 hasConcept C119599485 @default.
• W2007402269 hasConcept C123894998 @default.
• W2007402269 hasConcept C12554922 @default.
• W2007402269 hasConcept C127413603 @default.
• W2007402269 hasConcept C142613039 @default.
• W2007402269 hasConcept C185592680 @default.
• W2007402269 hasConcept C204328495 @default.
• W2007402269 hasConcept C2776545253 @default.
• W2007402269 hasConcept C2777576037 @default.
• W2007402269 hasConcept C2779222958 @default.
• W2007402269 hasConcept C40767141 @default.
• W2007402269 hasConcept C547475151 @default.
• W2007402269 hasConcept C55493867 @default.
• W2007402269 hasConcept C86803240 @default.
• W2007402269 hasConcept C95444343 @default.
• W2007402269 hasConceptScore W2007402269C104317684 @default.
• W2007402269 hasConceptScore W2007402269C119599485 @default.
• W2007402269 hasConceptScore W2007402269C123894998 @default.
• W2007402269 hasConceptScore W2007402269C12554922 @default.
• W2007402269 hasConceptScore W2007402269C127413603 @default.
• W2007402269 hasConceptScore W2007402269C142613039 @default.
• W2007402269 hasConceptScore W2007402269C185592680 @default.
• W2007402269 hasConceptScore W2007402269C204328495 @default.
• W2007402269 hasConceptScore W2007402269C2776545253 @default.

• next