Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007404424> ?p ?o ?g. }
- W2007404424 endingPage "7309" @default.
- W2007404424 startingPage "7305" @default.
- W2007404424 abstract "Antisense oligonucleotides are widely used as inhibitors of gene expression in cultured cells and have been proposed as potential therapeutic agents, but it is not known to what extent they are specific for their intended target RNAs. Statistical considerations indicate that if oligonucleotides can form hybrids with mRNA molecules in vivo by means of short or imperfect regions of complementarity, then the specificity of oligonucleotides as antisense reagents will be greatly compromised. We have used Xenopus oocytes as a model system in which to investigate the potential specificity of antisense oligonucleotides in vivo. We injected perfect and partially matched antisense oligonucleotides into oocytes and measured the resulting degradation of the target RNA in each case. On the basis of the extent to which antisense oligonucleotides can cause cleavage of RNAs at imperfectly matched target sites, we conclude that in this system it is probably not possible to obtain specific cleavage of an intended target RNA without also causing at least the partial destruction of many nontargeted RNAs." @default.
- W2007404424 created "2016-06-24" @default.
- W2007404424 creator A5012942110 @default.
- W2007404424 creator A5035484404 @default.
- W2007404424 creator A5089719674 @default.
- W2007404424 date "1992-08-15" @default.
- W2007404424 modified "2023-10-18" @default.
- W2007404424 title "Specificity of antisense oligonucleotides in vivo." @default.
- W2007404424 cites W1971159711 @default.
- W2007404424 cites W1977335029 @default.
- W2007404424 cites W1977698865 @default.
- W2007404424 cites W1981884157 @default.
- W2007404424 cites W1984234491 @default.
- W2007404424 cites W1985965093 @default.
- W2007404424 cites W1987693307 @default.
- W2007404424 cites W1988787228 @default.
- W2007404424 cites W199140675 @default.
- W2007404424 cites W2004545420 @default.
- W2007404424 cites W2032572088 @default.
- W2007404424 cites W2038948621 @default.
- W2007404424 cites W2046291479 @default.
- W2007404424 cites W2071001795 @default.
- W2007404424 cites W2072794717 @default.
- W2007404424 cites W2074114445 @default.
- W2007404424 cites W2089543118 @default.
- W2007404424 cites W2130917153 @default.
- W2007404424 cites W2157521126 @default.
- W2007404424 cites W2157934217 @default.
- W2007404424 cites W2221907578 @default.
- W2007404424 cites W2410612368 @default.
- W2007404424 cites W48777568 @default.
- W2007404424 doi "https://doi.org/10.1073/pnas.89.16.7305" @default.
- W2007404424 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/49698" @default.
- W2007404424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1380154" @default.
- W2007404424 hasPublicationYear "1992" @default.
- W2007404424 type Work @default.
- W2007404424 sameAs 2007404424 @default.
- W2007404424 citedByCount "226" @default.
- W2007404424 countsByYear W20074044242012 @default.
- W2007404424 countsByYear W20074044242013 @default.
- W2007404424 countsByYear W20074044242014 @default.
- W2007404424 countsByYear W20074044242015 @default.
- W2007404424 countsByYear W20074044242017 @default.
- W2007404424 countsByYear W20074044242018 @default.
- W2007404424 countsByYear W20074044242019 @default.
- W2007404424 countsByYear W20074044242020 @default.
- W2007404424 countsByYear W20074044242021 @default.
- W2007404424 countsByYear W20074044242022 @default.
- W2007404424 countsByYear W20074044242023 @default.
- W2007404424 crossrefType "journal-article" @default.
- W2007404424 hasAuthorship W2007404424A5012942110 @default.
- W2007404424 hasAuthorship W2007404424A5035484404 @default.
- W2007404424 hasAuthorship W2007404424A5089719674 @default.
- W2007404424 hasBestOaLocation W20074044242 @default.
- W2007404424 hasConcept C104317684 @default.
- W2007404424 hasConcept C105580179 @default.
- W2007404424 hasConcept C129312508 @default.
- W2007404424 hasConcept C151730666 @default.
- W2007404424 hasConcept C153911025 @default.
- W2007404424 hasConcept C175156509 @default.
- W2007404424 hasConcept C202416437 @default.
- W2007404424 hasConcept C207001950 @default.
- W2007404424 hasConcept C2779535977 @default.
- W2007404424 hasConcept C43369102 @default.
- W2007404424 hasConcept C54355233 @default.
- W2007404424 hasConcept C67705224 @default.
- W2007404424 hasConcept C70721500 @default.
- W2007404424 hasConcept C86803240 @default.
- W2007404424 hasConcept C95444343 @default.
- W2007404424 hasConceptScore W2007404424C104317684 @default.
- W2007404424 hasConceptScore W2007404424C105580179 @default.
- W2007404424 hasConceptScore W2007404424C129312508 @default.
- W2007404424 hasConceptScore W2007404424C151730666 @default.
- W2007404424 hasConceptScore W2007404424C153911025 @default.
- W2007404424 hasConceptScore W2007404424C175156509 @default.
- W2007404424 hasConceptScore W2007404424C202416437 @default.
- W2007404424 hasConceptScore W2007404424C207001950 @default.
- W2007404424 hasConceptScore W2007404424C2779535977 @default.
- W2007404424 hasConceptScore W2007404424C43369102 @default.
- W2007404424 hasConceptScore W2007404424C54355233 @default.
- W2007404424 hasConceptScore W2007404424C67705224 @default.
- W2007404424 hasConceptScore W2007404424C70721500 @default.
- W2007404424 hasConceptScore W2007404424C86803240 @default.
- W2007404424 hasConceptScore W2007404424C95444343 @default.
- W2007404424 hasIssue "16" @default.
- W2007404424 hasLocation W20074044241 @default.
- W2007404424 hasLocation W20074044242 @default.
- W2007404424 hasLocation W20074044243 @default.
- W2007404424 hasLocation W20074044244 @default.
- W2007404424 hasOpenAccess W2007404424 @default.
- W2007404424 hasPrimaryLocation W20074044241 @default.
- W2007404424 hasRelatedWork W1906502852 @default.
- W2007404424 hasRelatedWork W2013167593 @default.
- W2007404424 hasRelatedWork W2037714379 @default.
- W2007404424 hasRelatedWork W2039152596 @default.
- W2007404424 hasRelatedWork W2042426634 @default.
- W2007404424 hasRelatedWork W2075414118 @default.
- W2007404424 hasRelatedWork W2143718816 @default.