FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007406685> ?p ?o ?g. }

• W2007406685 endingPage "1301" @default.
• W2007406685 startingPage "1291" @default.
• W2007406685 abstract "PEGylation of therapeutic proteins is commonly used to extend half-lives and to reduce immunogenicity. However, reports of antibodies toward PEGylated proteins and of poly(ethylene glycol) (PEG) accumulation suggest that efficacy and safety concerns may arise. To understand the relationship among the pharmacology, immunogenicity, and toxicology of PEGylated proteins, we require knowledge of the disposition and metabolic fate of both the drug and the polymer moieties. The analysis of PEG by standard spectrophotometric or mass spectrometric techniques is problematic. Consequently, we have examined and compared two independent analytical approaches, based on gel electrophoresis and nuclear magnetic resonance (NMR) spectroscopy, to determine the biological fate of a model PEGylated protein, 40KPEG-insulin, within a rat model. Both immunoblotting with an antibody to PEG and NMR analyses (LOD 0.5 μg/mL for both assays) indicated that the PEG moiety remained detectable for several weeks in both serum and urine following intravenous administration of 40KPEG-insulin (4 mg/kg). In contrast, Western blotting with anti-insulin IgG indicated that the terminal half-life of the insulin moiety was far shorter than that of the PEG, providing clear evidence of conjugate cleavage. The application of combined analytical techniques in this way thus allows simultaneous independent monitoring of both protein and polymer elements of a PEGylated molecule. These methodologies also provide direct evidence for cleavage and definition of the chemical species present in biological fluids which may have toxicological consequences due to unconjugated PEG accumulation or immunogenic recognition of the uncoupled protein." @default.
• W2007406685 created "2016-06-24" @default.
• W2007406685 creator A5004511344 @default.
• W2007406685 creator A5006494530 @default.
• W2007406685 creator A5006874876 @default.
• W2007406685 creator A5012773581 @default.
• W2007406685 creator A5015164646 @default.
• W2007406685 creator A5016743800 @default.
• W2007406685 creator A5023569261 @default.
• W2007406685 creator A5083837295 @default.
• W2007406685 date "2012-04-18" @default.
• W2007406685 modified "2023-09-27" @default.
• W2007406685 title "Evidence for Metabolic Cleavage of a PEGylated Protein <i>in Vivo</i> Using Multiple Analytical Methodologies" @default.
• W2007406685 cites W1490299254 @default.
• W2007406685 cites W1555210583 @default.
• W2007406685 cites W1918944513 @default.
• W2007406685 cites W1967523778 @default.
• W2007406685 cites W1970266799 @default.
• W2007406685 cites W1987119060 @default.
• W2007406685 cites W2007102329 @default.
• W2007406685 cites W2008138329 @default.
• W2007406685 cites W2009510047 @default.
• W2007406685 cites W2010443042 @default.
• W2007406685 cites W2018808382 @default.
• W2007406685 cites W2022234371 @default.
• W2007406685 cites W2022453274 @default.
• W2007406685 cites W2024016060 @default.
• W2007406685 cites W2037808957 @default.
• W2007406685 cites W2048283280 @default.
• W2007406685 cites W2060860123 @default.
• W2007406685 cites W2065876461 @default.
• W2007406685 cites W2082556053 @default.
• W2007406685 cites W2082877367 @default.
• W2007406685 cites W2102778972 @default.
• W2007406685 cites W2110174460 @default.
• W2007406685 cites W2122290258 @default.
• W2007406685 cites W2150417402 @default.
• W2007406685 cites W2255928367 @default.
• W2007406685 cites W2612281319 @default.
• W2007406685 cites W2977710825 @default.
• W2007406685 cites W2987005477 @default.
• W2007406685 cites W4253535645 @default.
• W2007406685 cites W80378057 @default.
• W2007406685 cites W2092998911 @default.
• W2007406685 doi "https://doi.org/10.1021/mp200587m" @default.
• W2007406685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22480236" @default.
• W2007406685 hasPublicationYear "2012" @default.
• W2007406685 type Work @default.
• W2007406685 sameAs 2007406685 @default.
• W2007406685 citedByCount "28" @default.
• W2007406685 countsByYear W20074066852013 @default.
• W2007406685 countsByYear W20074066852014 @default.
• W2007406685 countsByYear W20074066852015 @default.
• W2007406685 countsByYear W20074066852016 @default.
• W2007406685 countsByYear W20074066852017 @default.
• W2007406685 countsByYear W20074066852018 @default.
• W2007406685 countsByYear W20074066852019 @default.
• W2007406685 countsByYear W20074066852021 @default.
• W2007406685 countsByYear W20074066852022 @default.
• W2007406685 countsByYear W20074066852023 @default.
• W2007406685 crossrefType "journal-article" @default.
• W2007406685 hasAuthorship W2007406685A5004511344 @default.
• W2007406685 hasAuthorship W2007406685A5006494530 @default.
• W2007406685 hasAuthorship W2007406685A5006874876 @default.
• W2007406685 hasAuthorship W2007406685A5012773581 @default.
• W2007406685 hasAuthorship W2007406685A5015164646 @default.
• W2007406685 hasAuthorship W2007406685A5016743800 @default.
• W2007406685 hasAuthorship W2007406685A5023569261 @default.
• W2007406685 hasAuthorship W2007406685A5083837295 @default.
• W2007406685 hasConcept C10138342 @default.
• W2007406685 hasConcept C134306372 @default.
• W2007406685 hasConcept C150903083 @default.
• W2007406685 hasConcept C159654299 @default.
• W2007406685 hasConcept C162324750 @default.
• W2007406685 hasConcept C178790620 @default.
• W2007406685 hasConcept C185592680 @default.
• W2007406685 hasConcept C197336794 @default.
• W2007406685 hasConcept C203014093 @default.
• W2007406685 hasConcept C207001950 @default.
• W2007406685 hasConcept C2776964284 @default.
• W2007406685 hasConcept C2777516009 @default.
• W2007406685 hasConcept C2779149719 @default.
• W2007406685 hasConcept C2780868878 @default.
• W2007406685 hasConcept C33923547 @default.
• W2007406685 hasConcept C43617362 @default.
• W2007406685 hasConcept C54400483 @default.
• W2007406685 hasConcept C55493867 @default.
• W2007406685 hasConcept C86803240 @default.
• W2007406685 hasConcept C98274493 @default.
• W2007406685 hasConceptScore W2007406685C10138342 @default.
• W2007406685 hasConceptScore W2007406685C134306372 @default.
• W2007406685 hasConceptScore W2007406685C150903083 @default.
• W2007406685 hasConceptScore W2007406685C159654299 @default.
• W2007406685 hasConceptScore W2007406685C162324750 @default.
• W2007406685 hasConceptScore W2007406685C178790620 @default.
• W2007406685 hasConceptScore W2007406685C185592680 @default.
• W2007406685 hasConceptScore W2007406685C197336794 @default.
• W2007406685 hasConceptScore W2007406685C203014093 @default.

• next