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• W2007422013 abstract "The isolation and characterization of six pigment-protein complexes from five diatom species (Phaeodactylum tricornutum Bohlin, Chaetoceros gracilis Schutt, Nitzschia sp. Mono Lake, Nitzschia laevis Hust. and Thalassiosira pseudonana (Hust.) Hasle and Heimdal) was accomplished by membrane dissociation with digitonin followed by gel electrophoresis. Six analogous complexes obtained from all species were correlated in spectral characteristics and relative mass with complexes from higher plants obtained by the same procedure. The largest of these complexes, comprising about 15% of the total Chl a, contained reaction centers of Photosystem I (PSI) and antenna pigments (LHC1).Some PSI complexes also separated from LHC1 in the gel. For the first time in diatoms, a Photosystem II complex was isolated and identified from its position in the gels, absorption and fluorescence spectra, lack of P700, and enrichment carotene. Three antenna pigment-protein complexes in addition to LHC1 occurred in varying proportion under different experimental conditions but in sum, they accounted for 70% of the total Chl a. All three bands were highly enriched in Chl c and fucoxanthin, although the ratio of Chl c/ xanthophyll decreased between the slowest migrating LHC2 and fastest moving LHC4 LHC3 contained the highest proportio of pigment-protein and was composed primarily of polypeptides of about 18,000 D. Essentially all α- and β-carotene was bound to the reaction center complexes. The Nitzschia from Mono Lake differed from the other species in that PSI complexes could not be readily dissociated from its membrane by digitonin treatment, a characteristic which may reflect a different chloroplast membrane structure in this alga." @default.
• W2007422013 created "2016-06-24" @default.
• W2007422013 creator A5077666579 @default.
• W2007422013 date "2008-03-31" @default.
• W2007422013 modified "2023-10-16" @default.
• W2007422013 title "PHOTOSYNTHETIC PIGMENT ORGANIZATION IN DIATOMS (BACILLARIOPHYCEAE)1" @default.
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• W2007422013 doi "https://doi.org/10.1111/j.1529-8817.1988.tb04460.x" @default.
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