Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007444724> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2007444724 endingPage "186" @default.
- W2007444724 startingPage "173" @default.
- W2007444724 abstract "Yeasts of the genus Zygosaccharomyces are notable agents of large-scale food spoilage. Despite the economic importance of these organisms, little is known about the stress adaptations whereby they adapt to many of the more severe conditions of food preservation. In this study it was shown that genes of Z. bailii, a yeast notable for its high resistances to food preservatives and ethanol, can be isolated by complementation of the corresponding mutant strains of Saccharomyces cerevisiae. It was also discovered that the acquisition by S. cerevisiae of a single small Z. bailii gene (ZbYME2) was sufficient for the former yeast to acquire the ability to degrade two major food preservatives, benzoic acid and sorbic acid. Using DNA cassettes containing dominant selectable markers and methods originally developed for performing gene deletions in S. cerevisiae, the two copies of ZbYME2 in the Z. bailii genome were sequentially deleted. The resulting Zbyme2/Zbyme2 homozygous deletant strain had lost any ability to utilize benzoate as sole carbon source and was more sensitive to weak acid preservatives during growth on glucose. Thus, ZbYME2, probably the nuclear gene for a mitochondrial mono-oxygenase function, is essential for Z. bailii to degrade food preservatives. This ability to catabolize weak acid preservatives is a significant factor contributing to the preservative resistance of Z. bailii under aerobic conditions. This study is the first to demonstrate that it is possible to delete in Z. bailii genes that are suspected as being important for growth of this organism in preserved foods and beverages. With the construction of further mutant of Z. bailii strains, a clearer picture should emerge of how this yeast adapts to the conditions of food preservation. This information will, in turn, allow the design of new preservation strategies. GenBank Accession Nos: ZbURA3 (AF279259), ZbTIM9 (AF279260), ZbYME2 (AF279261), ZbTRP1 (AF279262), ZbHHT1(AF296170)." @default.
- W2007444724 created "2016-06-24" @default.
- W2007444724 creator A5009360938 @default.
- W2007444724 creator A5058621466 @default.
- W2007444724 date "2001-01-01" @default.
- W2007444724 modified "2023-09-29" @default.
- W2007444724 title "Targeted gene deletion inZygosaccharomyces bailii" @default.
- W2007444724 cites W1823160263 @default.
- W2007444724 cites W1850569493 @default.
- W2007444724 cites W1893995452 @default.
- W2007444724 cites W1967390330 @default.
- W2007444724 cites W1992923868 @default.
- W2007444724 cites W1992979426 @default.
- W2007444724 cites W1997859335 @default.
- W2007444724 cites W2004595470 @default.
- W2007444724 cites W2005641752 @default.
- W2007444724 cites W2011534774 @default.
- W2007444724 cites W2013056827 @default.
- W2007444724 cites W2025915645 @default.
- W2007444724 cites W2047439787 @default.
- W2007444724 cites W2058077723 @default.
- W2007444724 cites W2062796799 @default.
- W2007444724 cites W2063646359 @default.
- W2007444724 cites W2067724938 @default.
- W2007444724 cites W2070756319 @default.
- W2007444724 cites W2079458070 @default.
- W2007444724 cites W2100057937 @default.
- W2007444724 cites W2114358611 @default.
- W2007444724 cites W2140227204 @default.
- W2007444724 cites W2140935709 @default.
- W2007444724 cites W2159408806 @default.
- W2007444724 cites W2169333620 @default.
- W2007444724 cites W4211043816 @default.
- W2007444724 cites W4211204307 @default.
- W2007444724 cites W93963094 @default.
- W2007444724 doi "https://doi.org/10.1002/1097-0061(20010130)18:2<173::aid-yea663>3.0.co;2-f" @default.
- W2007444724 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11169759" @default.
- W2007444724 hasPublicationYear "2001" @default.
- W2007444724 type Work @default.
- W2007444724 sameAs 2007444724 @default.
- W2007444724 citedByCount "41" @default.
- W2007444724 countsByYear W20074447242014 @default.
- W2007444724 countsByYear W20074447242015 @default.
- W2007444724 countsByYear W20074447242016 @default.
- W2007444724 countsByYear W20074447242017 @default.
- W2007444724 countsByYear W20074447242019 @default.
- W2007444724 countsByYear W20074447242022 @default.
- W2007444724 crossrefType "journal-article" @default.
- W2007444724 hasAuthorship W2007444724A5009360938 @default.
- W2007444724 hasAuthorship W2007444724A5058621466 @default.
- W2007444724 hasBestOaLocation W20074447241 @default.
- W2007444724 hasConcept C104317684 @default.
- W2007444724 hasConcept C143065580 @default.
- W2007444724 hasConcept C147490202 @default.
- W2007444724 hasConcept C188082640 @default.
- W2007444724 hasConcept C2777576037 @default.
- W2007444724 hasConcept C2779222958 @default.
- W2007444724 hasConcept C2779749926 @default.
- W2007444724 hasConcept C31903555 @default.
- W2007444724 hasConcept C523546767 @default.
- W2007444724 hasConcept C54355233 @default.
- W2007444724 hasConcept C55493867 @default.
- W2007444724 hasConcept C67031304 @default.
- W2007444724 hasConcept C86803240 @default.
- W2007444724 hasConceptScore W2007444724C104317684 @default.
- W2007444724 hasConceptScore W2007444724C143065580 @default.
- W2007444724 hasConceptScore W2007444724C147490202 @default.
- W2007444724 hasConceptScore W2007444724C188082640 @default.
- W2007444724 hasConceptScore W2007444724C2777576037 @default.
- W2007444724 hasConceptScore W2007444724C2779222958 @default.
- W2007444724 hasConceptScore W2007444724C2779749926 @default.
- W2007444724 hasConceptScore W2007444724C31903555 @default.
- W2007444724 hasConceptScore W2007444724C523546767 @default.
- W2007444724 hasConceptScore W2007444724C54355233 @default.
- W2007444724 hasConceptScore W2007444724C55493867 @default.
- W2007444724 hasConceptScore W2007444724C67031304 @default.
- W2007444724 hasConceptScore W2007444724C86803240 @default.
- W2007444724 hasIssue "2" @default.
- W2007444724 hasLocation W20074447241 @default.
- W2007444724 hasLocation W20074447242 @default.
- W2007444724 hasOpenAccess W2007444724 @default.
- W2007444724 hasPrimaryLocation W20074447241 @default.
- W2007444724 hasRelatedWork W2043251826 @default.
- W2007444724 hasRelatedWork W2081690701 @default.
- W2007444724 hasRelatedWork W2114217399 @default.
- W2007444724 hasRelatedWork W2117110650 @default.
- W2007444724 hasRelatedWork W2128665526 @default.
- W2007444724 hasRelatedWork W2602599566 @default.
- W2007444724 hasRelatedWork W3030185603 @default.
- W2007444724 hasRelatedWork W4280567361 @default.
- W2007444724 hasRelatedWork W4312201471 @default.
- W2007444724 hasRelatedWork W4378834801 @default.
- W2007444724 hasVolume "18" @default.
- W2007444724 isParatext "false" @default.
- W2007444724 isRetracted "false" @default.
- W2007444724 magId "2007444724" @default.
- W2007444724 workType "article" @default.