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• W2007453096 abstract "Enterotoxigenic Escherichia coli (ETEC) is an important pathogen responsible for secretory diarrhoea. The production of heat labile enterotoxin (LT), by ETEC, is largely responsible for the pathogenesis of diarrhoea. In the present study we investigated the effect of stress factors such as temperature, pH, osmotic stress and nutritional limitation on the production of LT by ETEC using in-house GMI-ELISA. Four strains of E. coli consisting, one standard strain MTCC 723 and three clinical isolates were used in the study. Maximum amount of LT (OD 3.285) was produced at 37°C followed by 40°C (OD 3.305). Growth of E. coli in medium with pH 8.6 resulted in maximum amount of LT production (OD 3.489). LT was not detectable when bacteria were grown in medium with pH ≤7.2 and ≥9.2. Sodium chloride concentration of 0.2 M stimulated maximum amount of LT production. Maximum amount of LT was produced when the bacteria were grown in medium containing 2.5g/l of glucose. All the stress factors had a significant effect on the LT production by E. coli, though quantitative differences in the various strains were observed." @default.
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• W2007453096 date "2009-10-01" @default.
• W2007453096 modified "2023-09-30" @default.
• W2007453096 title "EFFECT OF STRESS ON PRODUCTION OF HEAT LABILE ENTEROTOXIN BY ESCHERICHIA COLI" @default.
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• W2007453096 doi "https://doi.org/10.4103/0255-0857.55446" @default.
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