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• W2007456064 abstract "Abstract Aim The primary aim of this study was to identify the effects of hyperammonaemia on functional expression of Ca v 1.2 L‐type Ca 2+ channels in astroglia. Methods Primary cultures of mouse astrocytes were used to study effects of chronic treatment (1–5 days) with ammonium chloride, at 1, 3 and 5 m m on depolarization‐induced increases in free cytosolic Ca 2+ concentration ([Ca 2+ ] i , measured with Fura‐2 based microfluorimetry) in control conditions and following treatment with the L‐type Ca 2+ channel inhibitor, nifedipine, or with ryanodine receptor inhibitor, ryanodine. Expression of Ca v 1.2 mRNA was identified with RT ‐ PCR , whereas protein content was determined by Western blotting. Sustained hyperammonaemia in vivo was induced by daily injections of urease (33 units kg body weight −1 , i.p.) for 3 days. Results Depolarization‐induced [Ca 2+ ] i transients sensitive to nifedipine (peak of the response) and to ryanodine (plateau phase) were significantly increased in astrocytes chronically exposed to ammonium. The ammonium‐induced increase in Ca 2+ influx in astrocytes resulted from an upregulation of Ca v 1.2 channel's expression detected at mRNA and protein levels. Increase in Ca v 1.2 expression was prevented by ouabain antagonist canrenone. Similar upregulation of Ca v 1.2 gene expression was found in the brains of adult mice subjected to intraperitoneal injection of urease. In transgenic mice tagged with an astrocyte‐specific or neurone‐specific markers and treated with intraperitoneal injections of urease, the fluorescence‐activated cell sorting of neurones and astrocytes demonstrated that Ca v 1.2 mRNA expression was upregulated in astrocytes, but not in neurones. Conclusions Ammonium‐induced deregulation of astroglial Ca 2+ signalling, is, in part, associated with upregulation of Ca v 1.2 L‐type calcium channels." @default.
• W2007456064 created "2016-06-24" @default.
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• W2007456064 date "2015-04-18" @default.
• W2007456064 modified "2023-10-10" @default.
• W2007456064 title "Ammonium increases Ca²⁺ signalling and upregulates expression of Ca_v1.2 gene in astrocytes in primary cultures and in the <i>in vivo</i> brain" @default.
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• W2007456064 doi "https://doi.org/10.1111/apha.12500" @default.
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