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- W2007459062 abstract "Abstract The structure of simian virus 40 chromatin in nuclei isolated from infected monkey cells was probed with DNAase I. We show that a stretch of 390 base-pairs of SV40 DNA from nucleotide 5210 (± 5) to nucleotide 360 (± 15) is preferentially attacked by this enzyme. This region corresponds to the nucleosome-free region that has been shown previously to be involved in the control of early and late gene transcription, and includes the origin of replication. The DNAase I sensitivity in this region is not homogeneous: six hypersensitive sites were mapped. Three of these sites are separated from the others by a relatively resistant segment. Experiments with tsA and tsB mutants of SV40 that impair T antigen or major capsid protein functions, at the non-permissive temperature, suggest that the hypersensitivity pattern is not defined by these viral proteins." @default.
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- W2007459062 date "1982-09-01" @default.
- W2007459062 modified "2023-10-14" @default.
- W2007459062 title "Fine structure of the regulatory region of simian virus 40 minichromosomes revealed by DNAase I digestion" @default.
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- W2007459062 doi "https://doi.org/10.1016/0022-2836(82)90171-1" @default.
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