Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007484217> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2007484217 endingPage "7868" @default.
- W2007484217 startingPage "7860" @default.
- W2007484217 abstract "A mouse monoclonal anti-7-(O-carboxymethyl)oximinoestradiol antibody was photoaffinity labeled with two cross-reactive 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amido[17alpha-3H]estradiol photoreagents (6alpha- and 6beta-ANBA-[17alpha-3H]estradiol). Covalently bound radioactivity was found exclusively on the light chain. The maximal level of specific incorporation was 0.18 mol of label per mole of antibody for both photoreagents. In both cases, tryptic digestion of the photolabeled light chain, immunopurification with the immobilized antibody, reverse-phase liquid chromatography, and Edman degradation showed the presence of radioactive peptide GLM-([3H]X)-HGNTLEDGIPSR derived from peptide 46-61 of the light chain sequence (determined from cDNA) in which the unidentified amino acid corresponding to X is a Tyr residue. Two other radioactive peptides were also isolated, one corresponding probably to the methionine sulfoxide derivative of the peptide 46-61 photolabeled with the 6beta-reagent and the other to the N-terminal tetrapeptide 46-49 of the peptide 46-61 photolabeled with the 6alpha-reagent. In all cases, the main peak of radioactivity was released at the fourth Edman cycle, thus suggesting that the same Tyr-49 residue on the light chain was photolabeled. This residue is contiguous to the N-terminal amino acid of the second hypervariable complementary determining region 50-56 of light chain. Covalent labeling was confirmed by mass spectrometry of photolabeled peptides which showed molecular ion values corresponding to the addition of the photoactive 6alpha- or 6beta-ANBA-estradiol nitrene derivatives to the peptide." @default.
- W2007484217 created "2016-06-24" @default.
- W2007484217 creator A5005292755 @default.
- W2007484217 creator A5006098324 @default.
- W2007484217 creator A5008289247 @default.
- W2007484217 creator A5052392146 @default.
- W2007484217 creator A5052564121 @default.
- W2007484217 creator A5058829157 @default.
- W2007484217 creator A5067157462 @default.
- W2007484217 date "1997-06-01" @default.
- W2007484217 modified "2023-10-18" @default.
- W2007484217 title "Specific Photoaffinity Labeling of Tyr-49 on the Light Chain in the Steroid-Combining Site of a Mouse Monoclonal Anti-Estradiol Antibody Using Two Epimeric 6α- and 6β-(5-Azido-2-nitrobenzoyl)amidoestradiol Photoreagents<sup>,</sup>" @default.
- W2007484217 cites W1517594377 @default.
- W2007484217 cites W1557743033 @default.
- W2007484217 cites W1561244155 @default.
- W2007484217 cites W1858317139 @default.
- W2007484217 cites W1932052686 @default.
- W2007484217 cites W1976254721 @default.
- W2007484217 cites W2120513063 @default.
- W2007484217 cites W2152833618 @default.
- W2007484217 cites W2173256032 @default.
- W2007484217 cites W67864273 @default.
- W2007484217 doi "https://doi.org/10.1021/bi962645a" @default.
- W2007484217 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9201930" @default.
- W2007484217 hasPublicationYear "1997" @default.
- W2007484217 type Work @default.
- W2007484217 sameAs 2007484217 @default.
- W2007484217 citedByCount "13" @default.
- W2007484217 countsByYear W20074842172019 @default.
- W2007484217 countsByYear W20074842172022 @default.
- W2007484217 crossrefType "journal-article" @default.
- W2007484217 hasAuthorship W2007484217A5005292755 @default.
- W2007484217 hasAuthorship W2007484217A5006098324 @default.
- W2007484217 hasAuthorship W2007484217A5008289247 @default.
- W2007484217 hasAuthorship W2007484217A5052392146 @default.
- W2007484217 hasAuthorship W2007484217A5052564121 @default.
- W2007484217 hasAuthorship W2007484217A5058829157 @default.
- W2007484217 hasAuthorship W2007484217A5067157462 @default.
- W2007484217 hasConcept C104317684 @default.
- W2007484217 hasConcept C107824862 @default.
- W2007484217 hasConcept C159654299 @default.
- W2007484217 hasConcept C167625842 @default.
- W2007484217 hasConcept C181199279 @default.
- W2007484217 hasConcept C185592680 @default.
- W2007484217 hasConcept C203014093 @default.
- W2007484217 hasConcept C2776515853 @default.
- W2007484217 hasConcept C2778581200 @default.
- W2007484217 hasConcept C2779201268 @default.
- W2007484217 hasConcept C2779281246 @default.
- W2007484217 hasConcept C2781338088 @default.
- W2007484217 hasConcept C36394416 @default.
- W2007484217 hasConcept C43617362 @default.
- W2007484217 hasConcept C515207424 @default.
- W2007484217 hasConcept C55493867 @default.
- W2007484217 hasConcept C71240020 @default.
- W2007484217 hasConcept C86803240 @default.
- W2007484217 hasConcept C87933860 @default.
- W2007484217 hasConceptScore W2007484217C104317684 @default.
- W2007484217 hasConceptScore W2007484217C107824862 @default.
- W2007484217 hasConceptScore W2007484217C159654299 @default.
- W2007484217 hasConceptScore W2007484217C167625842 @default.
- W2007484217 hasConceptScore W2007484217C181199279 @default.
- W2007484217 hasConceptScore W2007484217C185592680 @default.
- W2007484217 hasConceptScore W2007484217C203014093 @default.
- W2007484217 hasConceptScore W2007484217C2776515853 @default.
- W2007484217 hasConceptScore W2007484217C2778581200 @default.
- W2007484217 hasConceptScore W2007484217C2779201268 @default.
- W2007484217 hasConceptScore W2007484217C2779281246 @default.
- W2007484217 hasConceptScore W2007484217C2781338088 @default.
- W2007484217 hasConceptScore W2007484217C36394416 @default.
- W2007484217 hasConceptScore W2007484217C43617362 @default.
- W2007484217 hasConceptScore W2007484217C515207424 @default.
- W2007484217 hasConceptScore W2007484217C55493867 @default.
- W2007484217 hasConceptScore W2007484217C71240020 @default.
- W2007484217 hasConceptScore W2007484217C86803240 @default.
- W2007484217 hasConceptScore W2007484217C87933860 @default.
- W2007484217 hasIssue "25" @default.
- W2007484217 hasLocation W20074842171 @default.
- W2007484217 hasLocation W20074842172 @default.
- W2007484217 hasOpenAccess W2007484217 @default.
- W2007484217 hasPrimaryLocation W20074842171 @default.
- W2007484217 hasRelatedWork W2006599802 @default.
- W2007484217 hasRelatedWork W2016062340 @default.
- W2007484217 hasRelatedWork W2050580465 @default.
- W2007484217 hasRelatedWork W2073199546 @default.
- W2007484217 hasRelatedWork W2073375101 @default.
- W2007484217 hasRelatedWork W2080726233 @default.
- W2007484217 hasRelatedWork W2119986142 @default.
- W2007484217 hasRelatedWork W2329310416 @default.
- W2007484217 hasRelatedWork W3140121548 @default.
- W2007484217 hasRelatedWork W435495287 @default.
- W2007484217 hasVolume "36" @default.
- W2007484217 isParatext "false" @default.
- W2007484217 isRetracted "false" @default.
- W2007484217 magId "2007484217" @default.
- W2007484217 workType "article" @default.