Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007486993> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2007486993 endingPage "644" @default.
- W2007486993 startingPage "640" @default.
- W2007486993 abstract "Secretion of a human collagen alpha1(I) chain fragment was achieved in Hansenula polymorpha using the native alpha1(I) procollagen secretory signal sequence. The N-terminal propeptide in the fragment was cleaved off during secretion, yielding the N-terminus of mature alpha1(I) collagen. In Pichia pastoris transformants, the expression of the fragment could be detected on RNA-level, but the product could not be determined extracellularly. After fusion of the fragment with a myc-HIS6 epitope, the intact product was found intracellularly. The difference in the extracellular level of the protein between the two expression hosts is most likely caused by difference in secretion efficiency." @default.
- W2007486993 created "2016-06-24" @default.
- W2007486993 creator A5006962003 @default.
- W2007486993 creator A5013826355 @default.
- W2007486993 creator A5014137521 @default.
- W2007486993 date "2000-06-01" @default.
- W2007486993 modified "2023-10-12" @default.
- W2007486993 title "Expression and secretion of human α1(I) procollagen fragment by Hansenula polymorpha as compared to Pichia pastoris" @default.
- W2007486993 cites W1987512033 @default.
- W2007486993 cites W1990163323 @default.
- W2007486993 cites W2004374675 @default.
- W2007486993 cites W2015020062 @default.
- W2007486993 cites W2023089746 @default.
- W2007486993 cites W2023853843 @default.
- W2007486993 cites W2026259690 @default.
- W2007486993 cites W2034309871 @default.
- W2007486993 cites W2034498656 @default.
- W2007486993 cites W2036078185 @default.
- W2007486993 cites W2037957091 @default.
- W2007486993 cites W2066958901 @default.
- W2007486993 cites W2092846883 @default.
- W2007486993 cites W2100837269 @default.
- W2007486993 cites W2195593402 @default.
- W2007486993 cites W4213151022 @default.
- W2007486993 doi "https://doi.org/10.1016/s0141-0229(00)00153-8" @default.
- W2007486993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10862867" @default.
- W2007486993 hasPublicationYear "2000" @default.
- W2007486993 type Work @default.
- W2007486993 sameAs 2007486993 @default.
- W2007486993 citedByCount "25" @default.
- W2007486993 countsByYear W20074869932012 @default.
- W2007486993 countsByYear W20074869932014 @default.
- W2007486993 countsByYear W20074869932015 @default.
- W2007486993 countsByYear W20074869932022 @default.
- W2007486993 countsByYear W20074869932023 @default.
- W2007486993 crossrefType "journal-article" @default.
- W2007486993 hasAuthorship W2007486993A5006962003 @default.
- W2007486993 hasAuthorship W2007486993A5013826355 @default.
- W2007486993 hasAuthorship W2007486993A5014137521 @default.
- W2007486993 hasConcept C104317684 @default.
- W2007486993 hasConcept C10858879 @default.
- W2007486993 hasConcept C153911025 @default.
- W2007486993 hasConcept C156399914 @default.
- W2007486993 hasConcept C159654299 @default.
- W2007486993 hasConcept C195616568 @default.
- W2007486993 hasConcept C203014093 @default.
- W2007486993 hasConcept C2779703480 @default.
- W2007486993 hasConcept C2781372317 @default.
- W2007486993 hasConcept C28406088 @default.
- W2007486993 hasConcept C40767141 @default.
- W2007486993 hasConcept C49039625 @default.
- W2007486993 hasConcept C55493867 @default.
- W2007486993 hasConcept C64348721 @default.
- W2007486993 hasConcept C86803240 @default.
- W2007486993 hasConceptScore W2007486993C104317684 @default.
- W2007486993 hasConceptScore W2007486993C10858879 @default.
- W2007486993 hasConceptScore W2007486993C153911025 @default.
- W2007486993 hasConceptScore W2007486993C156399914 @default.
- W2007486993 hasConceptScore W2007486993C159654299 @default.
- W2007486993 hasConceptScore W2007486993C195616568 @default.
- W2007486993 hasConceptScore W2007486993C203014093 @default.
- W2007486993 hasConceptScore W2007486993C2779703480 @default.
- W2007486993 hasConceptScore W2007486993C2781372317 @default.
- W2007486993 hasConceptScore W2007486993C28406088 @default.
- W2007486993 hasConceptScore W2007486993C40767141 @default.
- W2007486993 hasConceptScore W2007486993C49039625 @default.
- W2007486993 hasConceptScore W2007486993C55493867 @default.
- W2007486993 hasConceptScore W2007486993C64348721 @default.
- W2007486993 hasConceptScore W2007486993C86803240 @default.
- W2007486993 hasIssue "9-10" @default.
- W2007486993 hasLocation W20074869931 @default.
- W2007486993 hasLocation W20074869932 @default.
- W2007486993 hasOpenAccess W2007486993 @default.
- W2007486993 hasPrimaryLocation W20074869931 @default.
- W2007486993 hasRelatedWork W1971902798 @default.
- W2007486993 hasRelatedWork W2014728602 @default.
- W2007486993 hasRelatedWork W2050953035 @default.
- W2007486993 hasRelatedWork W2271499740 @default.
- W2007486993 hasRelatedWork W2350109756 @default.
- W2007486993 hasRelatedWork W2353156519 @default.
- W2007486993 hasRelatedWork W2383514277 @default.
- W2007486993 hasRelatedWork W2387947201 @default.
- W2007486993 hasRelatedWork W2412728054 @default.
- W2007486993 hasRelatedWork W3147115024 @default.
- W2007486993 hasVolume "26" @default.
- W2007486993 isParatext "false" @default.
- W2007486993 isRetracted "false" @default.
- W2007486993 magId "2007486993" @default.
- W2007486993 workType "article" @default.