Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007509103> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2007509103 endingPage "711" @default.
- W2007509103 startingPage "704" @default.
- W2007509103 abstract "Disease relapse is the leading cause of mortality for children diagnosed with disseminated neuroblastoma. The adoptive transfer of tumor-specific T cells is an attractive approach to target minimal residual disease following conventional therapies. We describe here the genetic engineering of human cytotoxic T lymphocytes (CTL) to express a chimeric immunoreceptor for re-directed HLA-independent recognition of neuroblastoma.The CE7R chimeric immunoreceptor was constructed by PCR splice overlap extension and is composed of a single-chain antibody extracellular domain (scFv) derived from the L1-CAM-specific murine CE7 hybridoma fused to human IgG1 hinge-Fc, the transmembrane portion of human CD4, and the cytoplasmic tail of huCD3-zeta chain (scFvFc:zeta). Primary human T cells were genetically modified by naked DNA electrotransfer of plasmid expression vector CE7R-pMG then analyzed by Western blotting, flow cytometry for CE7R expression and cell surface trafficking, 4-h chromium release assay for re-directed neuroblastoma lysis, and ELISA for tumor-specific activation of cytokine production.CE7R is expressed as an intact chimeric protein that trafficks to the cell surface as a type I transmembrane protein. Primary human CE7R-expressing CD8(+) CTL clones specifically recognize human neuroblastoma tumor cells and are activated for tumor cell lysis and T(c)1 cytokine production.These data demonstrate the utility of CE7R for re-directing the effector function of CTL to neuroblastoma and have provided the rationale to initiate a FDA-authorized (BB-IND#9149) pilot clinical trial to establish the feasibility and safety of adoptive transfer of autologous CE7R(+)CD8(+) CTL clones to children with recurrent/refractory neuroblastoma." @default.
- W2007509103 created "2016-06-24" @default.
- W2007509103 creator A5010964073 @default.
- W2007509103 creator A5013931986 @default.
- W2007509103 creator A5033591874 @default.
- W2007509103 creator A5058857048 @default.
- W2007509103 creator A5060347222 @default.
- W2007509103 creator A5086381895 @default.
- W2007509103 date "2004-05-26" @default.
- W2007509103 modified "2023-10-05" @default.
- W2007509103 title "Genetic engineering of cytolytic T lymphocytes for adoptive T-cell therapy of neuroblastoma" @default.
- W2007509103 doi "https://doi.org/10.1002/jgm.489" @default.
- W2007509103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15170741" @default.
- W2007509103 hasPublicationYear "2004" @default.
- W2007509103 type Work @default.
- W2007509103 sameAs 2007509103 @default.
- W2007509103 citedByCount "62" @default.
- W2007509103 countsByYear W20075091032012 @default.
- W2007509103 countsByYear W20075091032014 @default.
- W2007509103 countsByYear W20075091032015 @default.
- W2007509103 countsByYear W20075091032016 @default.
- W2007509103 countsByYear W20075091032017 @default.
- W2007509103 countsByYear W20075091032018 @default.
- W2007509103 countsByYear W20075091032019 @default.
- W2007509103 countsByYear W20075091032020 @default.
- W2007509103 countsByYear W20075091032021 @default.
- W2007509103 countsByYear W20075091032022 @default.
- W2007509103 countsByYear W20075091032023 @default.
- W2007509103 crossrefType "journal-article" @default.
- W2007509103 hasAuthorship W2007509103A5010964073 @default.
- W2007509103 hasAuthorship W2007509103A5013931986 @default.
- W2007509103 hasAuthorship W2007509103A5033591874 @default.
- W2007509103 hasAuthorship W2007509103A5058857048 @default.
- W2007509103 hasAuthorship W2007509103A5060347222 @default.
- W2007509103 hasAuthorship W2007509103A5086381895 @default.
- W2007509103 hasConcept C147969180 @default.
- W2007509103 hasConcept C153911025 @default.
- W2007509103 hasConcept C154317977 @default.
- W2007509103 hasConcept C167672396 @default.
- W2007509103 hasConcept C202751555 @default.
- W2007509103 hasConcept C203014093 @default.
- W2007509103 hasConcept C2776090121 @default.
- W2007509103 hasConcept C2776715637 @default.
- W2007509103 hasConcept C502942594 @default.
- W2007509103 hasConcept C54355233 @default.
- W2007509103 hasConcept C81885089 @default.
- W2007509103 hasConcept C86803240 @default.
- W2007509103 hasConcept C8891405 @default.
- W2007509103 hasConcept C90375314 @default.
- W2007509103 hasConceptScore W2007509103C147969180 @default.
- W2007509103 hasConceptScore W2007509103C153911025 @default.
- W2007509103 hasConceptScore W2007509103C154317977 @default.
- W2007509103 hasConceptScore W2007509103C167672396 @default.
- W2007509103 hasConceptScore W2007509103C202751555 @default.
- W2007509103 hasConceptScore W2007509103C203014093 @default.
- W2007509103 hasConceptScore W2007509103C2776090121 @default.
- W2007509103 hasConceptScore W2007509103C2776715637 @default.
- W2007509103 hasConceptScore W2007509103C502942594 @default.
- W2007509103 hasConceptScore W2007509103C54355233 @default.
- W2007509103 hasConceptScore W2007509103C81885089 @default.
- W2007509103 hasConceptScore W2007509103C86803240 @default.
- W2007509103 hasConceptScore W2007509103C8891405 @default.
- W2007509103 hasConceptScore W2007509103C90375314 @default.
- W2007509103 hasIssue "6" @default.
- W2007509103 hasLocation W20075091031 @default.
- W2007509103 hasLocation W20075091032 @default.
- W2007509103 hasOpenAccess W2007509103 @default.
- W2007509103 hasPrimaryLocation W20075091031 @default.
- W2007509103 hasRelatedWork W1513178816 @default.
- W2007509103 hasRelatedWork W1589818703 @default.
- W2007509103 hasRelatedWork W1743449791 @default.
- W2007509103 hasRelatedWork W1976572709 @default.
- W2007509103 hasRelatedWork W1992915380 @default.
- W2007509103 hasRelatedWork W2024677936 @default.
- W2007509103 hasRelatedWork W2042647947 @default.
- W2007509103 hasRelatedWork W2156631402 @default.
- W2007509103 hasRelatedWork W2480226770 @default.
- W2007509103 hasRelatedWork W2998930710 @default.
- W2007509103 hasVolume "6" @default.
- W2007509103 isParatext "false" @default.
- W2007509103 isRetracted "false" @default.
- W2007509103 magId "2007509103" @default.
- W2007509103 workType "article" @default.