FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007596689> ?p ?o ?g. }

• W2007596689 endingPage "71" @default.
• W2007596689 startingPage "67" @default.
• W2007596689 abstract "hCG is a heterodimer consisting of an alpha-subunit common among all members of the glycoprotein hormone family, LH, FSH, and TSH, and a unique beta-subunit responsible for receptor specificity. Biologically active single chain analogs of these hormones have been engineered in which the C-terminus of the beta-subunit was fused to the N-terminus of the alpha-subunit (N-beta-alpha-C) either with or without a linker such as the hCGbeta C-terminal peptide (CTP). This tandem order of subunits was chosen based on studies suggesting that the N-terminal region of hCGbeta and particularly the C-terminal region of the alpha-subunit are important in receptor binding and activation. Single chain hCG (YhCG1) can, in turn, be fused to the LH receptor to yield a hormone-receptor complex that is biologically active in transfected cells. Herein, we report the construction of a new single chain hCG analog (YhCG3) in which the C-terminus of the alpha-subunit is fused to the N-terminus of hCGbeta via a CTP (N-alpha-CTP-beta-C). Compared with YhCG1, this analog binds receptor with a 25- to 30-fold lower affinity, but, surprisingly, is capable of stimulating intracellular cAMP levels to the same extent. Furthermore, YhCG3 can be covalently linked to its receptor to produce a biologically active complex that results in elevated levels of basal cAMP in transfected cells. These results suggest that free N- and C-termini of hCGbeta and the alpha-subunit, respectively, are not essential for receptor binding and activation and that YhCG3 is in a more efficacious conformation for receptor activation than YhCG1." @default.
• W2007596689 created "2016-06-24" @default.
• W2007596689 creator A5055286743 @default.
• W2007596689 creator A5058522048 @default.
• W2007596689 creator A5085772314 @default.
• W2007596689 date "2000-01-01" @default.
• W2007596689 modified "2023-09-26" @default.
• W2007596689 title "A Biologically Active Single Chain Human Chorionic Gonadotropin Analog with Altered Receptor Binding Properties1" @default.
• W2007596689 cites W1515271009 @default.
• W2007596689 cites W1745812857 @default.
• W2007596689 cites W1971808451 @default.
• W2007596689 cites W1972051257 @default.
• W2007596689 cites W1976168422 @default.
• W2007596689 cites W1981573675 @default.
• W2007596689 cites W1989149712 @default.
• W2007596689 cites W2004682603 @default.
• W2007596689 cites W2024936974 @default.
• W2007596689 cites W2028612664 @default.
• W2007596689 cites W2050318820 @default.
• W2007596689 cites W2050971119 @default.
• W2007596689 cites W2072985378 @default.
• W2007596689 cites W2083116754 @default.
• W2007596689 cites W2091326377 @default.
• W2007596689 cites W2092974882 @default.
• W2007596689 cites W2102131608 @default.
• W2007596689 doi "https://doi.org/10.1210/endo.141.1.7275" @default.
• W2007596689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10614624" @default.
• W2007596689 hasPublicationYear "2000" @default.
• W2007596689 type Work @default.
• W2007596689 sameAs 2007596689 @default.
• W2007596689 citedByCount "19" @default.
• W2007596689 countsByYear W20075966892014 @default.
• W2007596689 countsByYear W20075966892015 @default.
• W2007596689 countsByYear W20075966892018 @default.
• W2007596689 countsByYear W20075966892019 @default.
• W2007596689 crossrefType "journal-article" @default.
• W2007596689 hasAuthorship W2007596689A5055286743 @default.
• W2007596689 hasAuthorship W2007596689A5058522048 @default.
• W2007596689 hasAuthorship W2007596689A5085772314 @default.
• W2007596689 hasBestOaLocation W20075966891 @default.
• W2007596689 hasConcept C104292427 @default.
• W2007596689 hasConcept C104317684 @default.
• W2007596689 hasConcept C116016267 @default.
• W2007596689 hasConcept C116073593 @default.
• W2007596689 hasConcept C121608353 @default.
• W2007596689 hasConcept C126322002 @default.
• W2007596689 hasConcept C133603901 @default.
• W2007596689 hasConcept C14053116 @default.
• W2007596689 hasConcept C153911025 @default.
• W2007596689 hasConcept C164083038 @default.
• W2007596689 hasConcept C167625842 @default.
• W2007596689 hasConcept C170493617 @default.
• W2007596689 hasConcept C185592680 @default.
• W2007596689 hasConcept C202751555 @default.
• W2007596689 hasConcept C206412730 @default.
• W2007596689 hasConcept C23589133 @default.
• W2007596689 hasConcept C36069631 @default.
• W2007596689 hasConcept C515207424 @default.
• W2007596689 hasConcept C530470458 @default.
• W2007596689 hasConcept C54009773 @default.
• W2007596689 hasConcept C55493867 @default.
• W2007596689 hasConcept C57711820 @default.
• W2007596689 hasConcept C71924100 @default.
• W2007596689 hasConcept C83730767 @default.
• W2007596689 hasConcept C86803240 @default.
• W2007596689 hasConceptScore W2007596689C104292427 @default.
• W2007596689 hasConceptScore W2007596689C104317684 @default.
• W2007596689 hasConceptScore W2007596689C116016267 @default.
• W2007596689 hasConceptScore W2007596689C116073593 @default.
• W2007596689 hasConceptScore W2007596689C121608353 @default.
• W2007596689 hasConceptScore W2007596689C126322002 @default.
• W2007596689 hasConceptScore W2007596689C133603901 @default.
• W2007596689 hasConceptScore W2007596689C14053116 @default.
• W2007596689 hasConceptScore W2007596689C153911025 @default.
• W2007596689 hasConceptScore W2007596689C164083038 @default.
• W2007596689 hasConceptScore W2007596689C167625842 @default.
• W2007596689 hasConceptScore W2007596689C170493617 @default.
• W2007596689 hasConceptScore W2007596689C185592680 @default.
• W2007596689 hasConceptScore W2007596689C202751555 @default.
• W2007596689 hasConceptScore W2007596689C206412730 @default.
• W2007596689 hasConceptScore W2007596689C23589133 @default.
• W2007596689 hasConceptScore W2007596689C36069631 @default.
• W2007596689 hasConceptScore W2007596689C515207424 @default.
• W2007596689 hasConceptScore W2007596689C530470458 @default.
• W2007596689 hasConceptScore W2007596689C54009773 @default.
• W2007596689 hasConceptScore W2007596689C55493867 @default.
• W2007596689 hasConceptScore W2007596689C57711820 @default.
• W2007596689 hasConceptScore W2007596689C71924100 @default.
• W2007596689 hasConceptScore W2007596689C83730767 @default.
• W2007596689 hasConceptScore W2007596689C86803240 @default.
• W2007596689 hasIssue "1" @default.
• W2007596689 hasLocation W20075966891 @default.
• W2007596689 hasLocation W20075966892 @default.
• W2007596689 hasOpenAccess W2007596689 @default.
• W2007596689 hasPrimaryLocation W20075966891 @default.
• W2007596689 hasRelatedWork W1536809633 @default.
• W2007596689 hasRelatedWork W1972642903 @default.
• W2007596689 hasRelatedWork W2000148336 @default.

• next