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- W2007628214 abstract "The complete nucleotide sequence of a 1957 bp DNA fragment containing the dihydrofolate reductase gene (DFRl) of the yeast Saccharomyces cerevisiae is presented. Within this region a single open reading frame of 633 base pairs was found which is capable of encoding a 211 amino acid residue protein with a calculated Mr of 24,233. The amino acid sequence derived from the yeast DFRl gene shows limited homology with sequences from both eukaryotic and non-eukaryotic DHFR enzymes. Northern blot hybridization reveals that the mRNA from this gene is a 900 base polyadenylated transcript. Yeast strains containing the cloned DFRl gene on multicopy number shuttle vector plasmids show dramatically enhanced methotrexate resistance. Consensus DNA sequences responsible for RNA polymerase II interaction and general amino acid control in S. cerevisiae are located within the 5'-noncoding region with respect to the open reading frame. The DNA fragment containing these sequences has been shown to be necessary for DFRl gene expression in both S. cerevisiae and E. coli." @default.
- W2007628214 created "2016-06-24" @default.
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- W2007628214 date "1987-01-01" @default.
- W2007628214 modified "2023-09-29" @default.
- W2007628214 title "Molecular characterization of the<i>Saccharomyces cerevisiae</i>dihydrofolate reductase gene (<i>DFRl</i>)" @default.
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- W2007628214 doi "https://doi.org/10.1093/nar/15.24.10355" @default.
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