Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007645277> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2007645277 abstract "We have previously reported an increased susceptibility to cell death of lymphocytes obtained from patients with Alzheimer's disease (AD) when exposed to oxidative stress induced by hydrogen peroxide (H2O2). Here we investigated whether the susceptibility to H 2 O 2 -induced death was related to the degree of dementia severity. Lymphocytes were extracted from peripheral blood from 25 AD patients (9 mild-moderate, CDR 1–2; 6 severe, CDR 3) and 10 healthy controls (all ≥ 60 years old) and exposed to H 2 O 2 for 20hrs - in the presence or absence of 5 mM 3-aminobenzamide (3-ABA), a PARP-1 inhibitor- or to staurosporine, an apoptosis inducer. Cell death was evaluated by flow cytometry and propidium iodide (PI) staining, whereby viable (PI-negative), apoptotic (PI-positive, hypodiploid) and necrotic (PI-positive diploid) cells were distinguished. The dose response curve of lymphocyte viability in the presence of H2O2 was shifted to the left in AD patients compared to healthy controls, with severe dementia showing the highest vulnerability to H 2 O 2 and those with mild to moderate dementia showing intermediate values; i.e. treatment with 50 μM H 2 O 2 (around LD50) for 20 hrs induced death of 68.1% of lymphocytes from patients with severe dementia, 51.1% of those with mild to moderate dementia, and 34.6% of healthy control lymphocytes. The greater susceptibility to death was due to an increase mostly of apoptosis. Staurosporine, an apoptosis inducer, at concentrations between 0.6 - 6mM provoked death to a similar extent in the three groups of patients. As previously shown, H 2 O 2 -induced death was significantly reduced by PARP inhibition, whereby the extent of protection was less significant in lymphocytes from patients with severe dementia." @default.
- W2007645277 created "2016-06-24" @default.
- W2007645277 creator A5023232121 @default.
- W2007645277 creator A5034268426 @default.
- W2007645277 creator A5035514608 @default.
- W2007645277 creator A5039658579 @default.
- W2007645277 creator A5040288625 @default.
- W2007645277 creator A5060520784 @default.
- W2007645277 creator A5061641514 @default.
- W2007645277 creator A5068771817 @default.
- W2007645277 creator A5090844447 @default.
- W2007645277 date "2013-07-01" @default.
- W2007645277 modified "2023-09-26" @default.
- W2007645277 title "P3-043: The increased susceptibility to oxidative death of lymphocytes from people with Alzheimer's disease correlates with the severity of dementia and is specific for oxidative damage" @default.
- W2007645277 doi "https://doi.org/10.1016/j.jalz.2013.05.1112" @default.
- W2007645277 hasPublicationYear "2013" @default.
- W2007645277 type Work @default.
- W2007645277 sameAs 2007645277 @default.
- W2007645277 citedByCount "0" @default.
- W2007645277 crossrefType "journal-article" @default.
- W2007645277 hasAuthorship W2007645277A5023232121 @default.
- W2007645277 hasAuthorship W2007645277A5034268426 @default.
- W2007645277 hasAuthorship W2007645277A5035514608 @default.
- W2007645277 hasAuthorship W2007645277A5039658579 @default.
- W2007645277 hasAuthorship W2007645277A5040288625 @default.
- W2007645277 hasAuthorship W2007645277A5060520784 @default.
- W2007645277 hasAuthorship W2007645277A5061641514 @default.
- W2007645277 hasAuthorship W2007645277A5068771817 @default.
- W2007645277 hasAuthorship W2007645277A5090844447 @default.
- W2007645277 hasConcept C126322002 @default.
- W2007645277 hasConcept C181199279 @default.
- W2007645277 hasConcept C190283241 @default.
- W2007645277 hasConcept C195794163 @default.
- W2007645277 hasConcept C203014093 @default.
- W2007645277 hasConcept C2775934118 @default.
- W2007645277 hasConcept C2776151105 @default.
- W2007645277 hasConcept C2777761686 @default.
- W2007645277 hasConcept C2779084600 @default.
- W2007645277 hasConcept C2779134260 @default.
- W2007645277 hasConcept C2779483572 @default.
- W2007645277 hasConcept C31573885 @default.
- W2007645277 hasConcept C55493867 @default.
- W2007645277 hasConcept C71924100 @default.
- W2007645277 hasConcept C86803240 @default.
- W2007645277 hasConceptScore W2007645277C126322002 @default.
- W2007645277 hasConceptScore W2007645277C181199279 @default.
- W2007645277 hasConceptScore W2007645277C190283241 @default.
- W2007645277 hasConceptScore W2007645277C195794163 @default.
- W2007645277 hasConceptScore W2007645277C203014093 @default.
- W2007645277 hasConceptScore W2007645277C2775934118 @default.
- W2007645277 hasConceptScore W2007645277C2776151105 @default.
- W2007645277 hasConceptScore W2007645277C2777761686 @default.
- W2007645277 hasConceptScore W2007645277C2779084600 @default.
- W2007645277 hasConceptScore W2007645277C2779134260 @default.
- W2007645277 hasConceptScore W2007645277C2779483572 @default.
- W2007645277 hasConceptScore W2007645277C31573885 @default.
- W2007645277 hasConceptScore W2007645277C55493867 @default.
- W2007645277 hasConceptScore W2007645277C71924100 @default.
- W2007645277 hasConceptScore W2007645277C86803240 @default.
- W2007645277 hasIssue "4S_Part_14" @default.
- W2007645277 hasLocation W20076452771 @default.
- W2007645277 hasOpenAccess W2007645277 @default.
- W2007645277 hasPrimaryLocation W20076452771 @default.
- W2007645277 hasRelatedWork W1968505653 @default.
- W2007645277 hasRelatedWork W2003699682 @default.
- W2007645277 hasRelatedWork W2019266055 @default.
- W2007645277 hasRelatedWork W2060065823 @default.
- W2007645277 hasRelatedWork W2060924028 @default.
- W2007645277 hasRelatedWork W2076104235 @default.
- W2007645277 hasRelatedWork W2166379079 @default.
- W2007645277 hasRelatedWork W2220797277 @default.
- W2007645277 hasRelatedWork W2328524531 @default.
- W2007645277 hasRelatedWork W2792118776 @default.
- W2007645277 hasVolume "9" @default.
- W2007645277 isParatext "false" @default.
- W2007645277 isRetracted "false" @default.
- W2007645277 magId "2007645277" @default.
- W2007645277 workType "article" @default.