Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007652052> ?p ?o ?g. }
- W2007652052 endingPage "398" @default.
- W2007652052 startingPage "390" @default.
- W2007652052 abstract "Archaerhodopsin 4 (AR4), a new member of the microbial rhodopsin family, is isolated from Halobacterium species xz515 in a Tibetan salt lake. AR4 functions as a proton pump similar to bacteriorhodopsin (BR) but with an opposite temporal order of proton uptake and release at neutral pH. However, further studies to elucidate the mechanism of the proton pump and photocycle of AR4 have been inhibited due to the difficulty of establishing a suitable system in which to express recombinant AR4 mutants. In this paper, we report a reliable method for expressing recombinant AR4 in Halobacterium salinarum L33 with a high yield of up to 20 mg/l. Experimental results show that the recombinant AR4 retains the light-driven proton pump characteristics and photo-cycling kinetics, similar to that in the native membrane. The functional role of bacterioruberin in AR4 and the trimeric packing of AR4 in its native and recombinant forms are investigated through light-induced kinetic measurements, two-dimensional solid-state NMR experiments, dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). Such approaches provide new insights into structure–function relationships of AR4, and form a basis for other archaeal rhodopsins." @default.
- W2007652052 created "2016-06-24" @default.
- W2007652052 creator A5007213740 @default.
- W2007652052 creator A5007947547 @default.
- W2007652052 creator A5013240931 @default.
- W2007652052 creator A5016570969 @default.
- W2007652052 creator A5026499832 @default.
- W2007652052 creator A5027059075 @default.
- W2007652052 creator A5064357764 @default.
- W2007652052 date "2015-04-01" @default.
- W2007652052 modified "2023-10-17" @default.
- W2007652052 title "Novel expression and characterization of a light driven proton pump archaerhodopsin 4 in a Halobacterium salinarum strain" @default.
- W2007652052 cites W1497394985 @default.
- W2007652052 cites W1533511716 @default.
- W2007652052 cites W1544485066 @default.
- W2007652052 cites W1558893130 @default.
- W2007652052 cites W187856331 @default.
- W2007652052 cites W1885054238 @default.
- W2007652052 cites W1964183942 @default.
- W2007652052 cites W1966910457 @default.
- W2007652052 cites W1969898499 @default.
- W2007652052 cites W1970405929 @default.
- W2007652052 cites W1977754106 @default.
- W2007652052 cites W1978315291 @default.
- W2007652052 cites W1980007786 @default.
- W2007652052 cites W1980239136 @default.
- W2007652052 cites W1981564460 @default.
- W2007652052 cites W1985074623 @default.
- W2007652052 cites W1989662761 @default.
- W2007652052 cites W1989695304 @default.
- W2007652052 cites W1989867387 @default.
- W2007652052 cites W1991229579 @default.
- W2007652052 cites W1994609674 @default.
- W2007652052 cites W1995139556 @default.
- W2007652052 cites W1995253643 @default.
- W2007652052 cites W1996468364 @default.
- W2007652052 cites W1997447018 @default.
- W2007652052 cites W1997759750 @default.
- W2007652052 cites W2002869713 @default.
- W2007652052 cites W2004649637 @default.
- W2007652052 cites W2004747713 @default.
- W2007652052 cites W2004991314 @default.
- W2007652052 cites W2005768010 @default.
- W2007652052 cites W2011368767 @default.
- W2007652052 cites W2012479522 @default.
- W2007652052 cites W2014050915 @default.
- W2007652052 cites W2016282681 @default.
- W2007652052 cites W2024305830 @default.
- W2007652052 cites W2025424550 @default.
- W2007652052 cites W2026701108 @default.
- W2007652052 cites W2027377567 @default.
- W2007652052 cites W2030374375 @default.
- W2007652052 cites W2031210068 @default.
- W2007652052 cites W2035688781 @default.
- W2007652052 cites W2038061512 @default.
- W2007652052 cites W2045458872 @default.
- W2007652052 cites W2048794698 @default.
- W2007652052 cites W2051752535 @default.
- W2007652052 cites W2051956840 @default.
- W2007652052 cites W2054939201 @default.
- W2007652052 cites W2059094913 @default.
- W2007652052 cites W2060524586 @default.
- W2007652052 cites W2062021082 @default.
- W2007652052 cites W2062594525 @default.
- W2007652052 cites W2063442912 @default.
- W2007652052 cites W2066435396 @default.
- W2007652052 cites W2068876377 @default.
- W2007652052 cites W2072417809 @default.
- W2007652052 cites W2074062743 @default.
- W2007652052 cites W2074337748 @default.
- W2007652052 cites W2077049950 @default.
- W2007652052 cites W2077306308 @default.
- W2007652052 cites W2079693190 @default.
- W2007652052 cites W2081409211 @default.
- W2007652052 cites W2086565576 @default.
- W2007652052 cites W2091687284 @default.
- W2007652052 cites W2097612723 @default.
- W2007652052 cites W2099775913 @default.
- W2007652052 cites W2105002900 @default.
- W2007652052 cites W2107118921 @default.
- W2007652052 cites W2107746113 @default.
- W2007652052 cites W2110152226 @default.
- W2007652052 cites W2110461335 @default.
- W2007652052 cites W2115357061 @default.
- W2007652052 cites W2119828149 @default.
- W2007652052 cites W2119951313 @default.
- W2007652052 cites W2124881855 @default.
- W2007652052 cites W2150605667 @default.
- W2007652052 cites W2151935555 @default.
- W2007652052 cites W2153258920 @default.
- W2007652052 cites W2155350436 @default.
- W2007652052 cites W2157322038 @default.
- W2007652052 cites W2165870630 @default.
- W2007652052 cites W2166591252 @default.
- W2007652052 cites W2171777099 @default.
- W2007652052 cites W2284719506 @default.
- W2007652052 cites W2313406892 @default.
- W2007652052 cites W4293247451 @default.