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- W2007662316 abstract "Abstract We have developed a rapid method to enzymatically disperse rat retinae and to separate large quantities of various cell types by centrifugation through a metrizamide density gradient. Seven distinct bands of cells were detected in the gradient (I–VII). Cells from layers III and V were morphologically identified as red blood cells and photoreceptor cells, respectively. [ 125 I]Horseradish peroxidase injected into the lateral geniculate bodies labels the ganglion cells via retrograde transport. This technique allowed us to demonstrate the presence of ganglion cells in layer I of the metrizamide gradient. This method will be useful for biochemical and pharmacological characterization of several types of retinal cells." @default.
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- W2007662316 date "1980-07-01" @default.
- W2007662316 modified "2023-09-25" @default.
- W2007662316 title "A simple and rapid method to isolate rat retinal cells for biochemical analysis" @default.
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- W2007662316 doi "https://doi.org/10.1016/0143-4179(80)90007-4" @default.
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