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• W2007662613 abstract "Alveolar epithelial type 2 cells (AEC2) isolated from hyperoxia-treated animals exhibit increases in both proliferation and DNA damage in response to culture. AEC2 express the zonula adherens proteins E-cadherin, α-, β- and γ-catenin, desmoglein, and pp120, as demonstrated by Western blotting. Immunohistochemical analysis of cultured AEC2 showed expression of E-cadherin on cytoplasmic membranes varying from strongly to weakly staining. When cultured AEC2 placed in suspension were labeled with fluorescent-tagged antibodies to E-cadherin, cells could be sorted into at least two subpopulations, either dim or brightly staining for this marker. With the use of antibody to E-cadherin bound to magnetic beads, cells were physically separated into E-cadherin-positive and -negative subpopulations, which were then analyzed for differences in proliferation and DNA damage. The E-cadherin-positive subpopulation contained the majority of damaged cells, was quiescent, and expressed low levels of telomerase activity, whereas the E-cadherin-negative subpopulation was undamaged, proliferative, and expressed high levels of telomerase activity." @default.
• W2007662613 created "2016-06-24" @default.
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• W2007662613 date "2004-04-01" @default.
• W2007662613 modified "2023-09-27" @default.
• W2007662613 title "Isolation of a putative progenitor subpopulation of alveolar epithelial type 2 cells" @default.
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• W2007662613 doi "https://doi.org/10.1152/ajplung.00159.2003" @default.
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