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• W2007672686 abstract "Background . Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods . At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M1><mml:mrow><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>25</mml:mn></mml:mrow></mml:math>; hAA-CHF). Minced tissues were suspended in complete (serum-containing) DMEM. Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit). Characterization of<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M2><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>cells included immunohistochemical screening with a panel of monoclonal antibodies. Results . Cells, including phase-bright cells identified as<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M3><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>, spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7. When cocultured with tissue, emigrated<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M4><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>cells proliferated, first as loosely attached clones and later as multicellular clusters. At Day 21 ~ 5% of cells were<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M5><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>. Between Days 14 and 28<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M6><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>cells exhibited mesodermal commitment (GATA-<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M7><mml:mrow><mml:msup><mml:mtext>4</mml:mtext><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>and<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M8><mml:mrow><mml:mtext>NKX2</mml:mtext><mml:msup><mml:mrow><mml:mtext>.5</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>); then after Day 28 cardiac lineages (<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M9><mml:mrow><mml:msup><mml:mrow><mml:mtext>flk-1</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>, smooth muscle<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M10><mml:mrow><mml:msup><mml:mrow><mml:mtext>actin</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>,<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M11><mml:mrow><mml:msup><mml:mrow><mml:mtext>troponin-I</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>, and myosin light<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M12><mml:mrow><mml:msup><mml:mrow><mml:mtext>chain</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>). Conclusions .<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M13><mml:mrow><mml:msup><mml:mrow><mml:mtext>C-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF. SEC is a novel culture method for derivation of migratory<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M14><mml:mrow><mml:msup><mml:mrow><mml:mtext>hc-kit</mml:mtext></mml:mrow><mml:mrow><mml:mtext>positive</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro ." @default.
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• W2007672686 date "2012-01-01" @default.
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• W2007672686 title "Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate" @default.
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