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- W2007675455 abstract "A set of monoclonal antibodies that recognizes a Trypanosoma cruzi 45-kDa protein was produced and used to characterize this molecule and study its role in trypanosome adhesion to heart myoblasts. We found that the 45-kDa protein is a surface mucin, is expressed only in invasive trypomastigotes, but not in noninvasive epimastigotes or amastigotes, and is released by the trypanosome in culture medium. One of the monoclonal antibodies (Mab B5) from this set inhibits the attachment of trypomastigotes to heart myoblasts preventing trypanosome entry, whereas the others (Mabs B4 and F1) do not. This inhibition was seen with the B5 hybridoma culture supernatant, with the purified Mab B5 IgG or with Mab B5 Fab fragments. These novel findings identify the 45-kDa mucin as a new T. cruzi ligand that is used by invasive forms of this organism to adhere to heart myoblasts." @default.
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- W2007675455 date "2002-01-01" @default.
- W2007675455 modified "2023-09-26" @default.
- W2007675455 title "Trypanosoma cruzi Uses a 45-kDa Mucin for Adhesion to Mammalian Cells" @default.
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- W2007675455 doi "https://doi.org/10.1006/bbrc.2001.6189" @default.
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