Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007686989> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2007686989 endingPage "207" @default.
- W2007686989 startingPage "195" @default.
- W2007686989 abstract "Cytokines are essential molecules throughout the development of the nervous system and also play an important role during the adult life span. In the present work, we analyzed in vitro the effect of spleen-cell-conditioned medium (SCM) on the survival and [3H]-choline uptake of neonatal rat retinal cells. SCM induced an increase in neuronal survival, glial cell proliferation and neurite outgrowth, as evaluated by biochemical and morphological criteria. These effects were time dependent; after 120 h, SCM induced a 6-fold increase in the total protein level. The effect of SCM was blocked both by the inhibition of protein tyrosine kinase activity (10 microM genistein) and by the inhibition of cell division (20 microM fluorodeoxyuridine). SCM also increased the uptake of [3H]-choline by retinal cells. The effect was time dependent. The maximum effect was obtained after 48 h and was maintained at a high level until 120 h. Treatment by 10 microM genistein and 15 microM bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM) (an intracellular calcium chelator) completely blocked this effect. However, 20 microM fluorodeoxyuridine did not abolish it. Conditioned medium obtained from glial cells stimulated with SCM (S-GCM) induced an effect on [3H]-choline uptake earlier than that promoted by SCM. Anti-interleukin-2 (IL-2) antibodies blocked the effect of both SCM and S-GCM on [3H]-choline uptake after 48 and 72 h. IL-2 (50 U/ml) elicited the same effect as that observed when the cells were maintained in the presence of SCM. Taken together, our results suggest that IL-2 plays an important role in controlling the survival and differentiation of retinal cells in vitro." @default.
- W2007686989 created "2016-06-24" @default.
- W2007686989 creator A5039680381 @default.
- W2007686989 creator A5057546103 @default.
- W2007686989 creator A5058786567 @default.
- W2007686989 creator A5085966780 @default.
- W2007686989 date "2000-01-01" @default.
- W2007686989 modified "2023-09-23" @default.
- W2007686989 title "Effect of Spleen-Cell-Conditioned Medium on [<sup>3</sup>H]-Choline Uptake by Retinal Cells in vitro Is Mediated by IL-2" @default.
- W2007686989 cites W1439176434 @default.
- W2007686989 cites W1664741673 @default.
- W2007686989 cites W1668518521 @default.
- W2007686989 cites W1775749144 @default.
- W2007686989 cites W1966639644 @default.
- W2007686989 cites W1976148134 @default.
- W2007686989 cites W1976877707 @default.
- W2007686989 cites W1983179971 @default.
- W2007686989 cites W1990285880 @default.
- W2007686989 cites W1995322850 @default.
- W2007686989 cites W1996294113 @default.
- W2007686989 cites W1997107997 @default.
- W2007686989 cites W1999920197 @default.
- W2007686989 cites W2000885272 @default.
- W2007686989 cites W2001024910 @default.
- W2007686989 cites W2006316186 @default.
- W2007686989 cites W2013822102 @default.
- W2007686989 cites W2014496326 @default.
- W2007686989 cites W2018527781 @default.
- W2007686989 cites W2018644594 @default.
- W2007686989 cites W2041071075 @default.
- W2007686989 cites W2043223456 @default.
- W2007686989 cites W2047064666 @default.
- W2007686989 cites W2047485097 @default.
- W2007686989 cites W2076355373 @default.
- W2007686989 cites W2077397331 @default.
- W2007686989 cites W2082597743 @default.
- W2007686989 cites W2090701428 @default.
- W2007686989 cites W2093165649 @default.
- W2007686989 cites W2093406879 @default.
- W2007686989 cites W2099879877 @default.
- W2007686989 cites W2109811799 @default.
- W2007686989 cites W2109957949 @default.
- W2007686989 cites W2314622952 @default.
- W2007686989 cites W2411045063 @default.
- W2007686989 cites W2991684622 @default.
- W2007686989 doi "https://doi.org/10.1159/000026439" @default.
- W2007686989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10810252" @default.
- W2007686989 hasPublicationYear "2000" @default.
- W2007686989 type Work @default.
- W2007686989 sameAs 2007686989 @default.
- W2007686989 citedByCount "7" @default.
- W2007686989 countsByYear W20076869892013 @default.
- W2007686989 countsByYear W20076869892016 @default.
- W2007686989 crossrefType "journal-article" @default.
- W2007686989 hasAuthorship W2007686989A5039680381 @default.
- W2007686989 hasAuthorship W2007686989A5057546103 @default.
- W2007686989 hasAuthorship W2007686989A5058786567 @default.
- W2007686989 hasAuthorship W2007686989A5085966780 @default.
- W2007686989 hasConcept C134018914 @default.
- W2007686989 hasConcept C153911025 @default.
- W2007686989 hasConcept C185592680 @default.
- W2007686989 hasConcept C2781123555 @default.
- W2007686989 hasConcept C55493867 @default.
- W2007686989 hasConcept C86803240 @default.
- W2007686989 hasConceptScore W2007686989C134018914 @default.
- W2007686989 hasConceptScore W2007686989C153911025 @default.
- W2007686989 hasConceptScore W2007686989C185592680 @default.
- W2007686989 hasConceptScore W2007686989C2781123555 @default.
- W2007686989 hasConceptScore W2007686989C55493867 @default.
- W2007686989 hasConceptScore W2007686989C86803240 @default.
- W2007686989 hasIssue "4" @default.
- W2007686989 hasLocation W20076869891 @default.
- W2007686989 hasLocation W20076869892 @default.
- W2007686989 hasOpenAccess W2007686989 @default.
- W2007686989 hasPrimaryLocation W20076869891 @default.
- W2007686989 hasRelatedWork W1552666152 @default.
- W2007686989 hasRelatedWork W1978406542 @default.
- W2007686989 hasRelatedWork W1978446484 @default.
- W2007686989 hasRelatedWork W1981928760 @default.
- W2007686989 hasRelatedWork W2045176963 @default.
- W2007686989 hasRelatedWork W2065892543 @default.
- W2007686989 hasRelatedWork W2341578418 @default.
- W2007686989 hasRelatedWork W2611969698 @default.
- W2007686989 hasRelatedWork W2748952813 @default.
- W2007686989 hasRelatedWork W2950650656 @default.
- W2007686989 hasVolume "7" @default.
- W2007686989 isParatext "false" @default.
- W2007686989 isRetracted "false" @default.
- W2007686989 magId "2007686989" @default.
- W2007686989 workType "article" @default.