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• W2007704010 abstract "1. We have studied the effects of purinoceptor stimulation on Ca2+ signals in bovine adrenomedullary endothelial cells. [Ca2+]i was determined with the fluorescent probe fura-2 both in population samples and in single, isolated, endothelial cells in primary culture and after subculturing. 2. In endothelial cells, maintained in culture for more than one passage, several purinoceptor agonists elicited clear [Ca2+]i transient peaks that remained in the absence of extracellular Ca2+. Adenosine 5'-triphosphate (ATP) and uridine 5'-triphosphate (UTP) were equipotently active, with EC50 values of 8.5 +/- 0.9 microM and 6.9 +/- 1.5 microM, respectively, whereas 2-methylthioadenosine 5'-triphosphate (2MeSATP), adenosine 5'-(alpha, beta-methylene)triphosphate (alpha, beta-MeATP) and adenosine(5')tetraphospho(5')adenosine (Ap4A) were basically inactive. Adenosine 5'-O-(2-thiodiphosphate) (ADP beta S) was a weak agonist. The apparent potency order was UTP = ATP > ADP beta S >> 2MeSATP > alpha, beta-MeATP. 3. Cross-desensitization experiments revealed that UTP or ATP, added sequentially at concentrations of maximal effect, could completely abolish the [Ca2+]i response to the second agonist. ADP beta S exerted only a partial desensitization of the response to maximal ATP, in accordance with its lower potency in raising [Ca2+]i. 4. The effect on [Ca2+]i of 100 microM ATP in subcultured cells was reduced by only 25% with 100 microM suramin pretreatment and was negligibly affected by exposure to 10 microM pyridoxalphosphate-6-azophenyl-2', 4'-disulphonic acid (PPADS). The concentration-effect curve for ATP was not significantly affected by PPADS, but was displaced to the right by a factor of 6.5 by 100 microM suramin. 5. In primary cultures, clear [Ca2+]i responses were elicited by 2MeSATP. Suramin totally and selectively blocked 2MeSATP responses, whereas UTP-evoked [Ca2+]i transients were mainly unaffected by suramin or PPADS. Over 80% of cells tested showed responses to both 2MeSATP and UTP. The [Ca2+]i response to UTP was not desensitized in the presence of 2MeSATP. 6. ATP and UTP stimulated the release of preloaded [3H]-arachidonic acid ([3H]-AA), both in the presence and in the absence of extracellular Ca2+, by approximately 135% with respect to basal levels. Suramin and PPADS enhanced, rather than inhibited, the [3H]-AA releasing effect of ATP by 2.5 times. Suramin also potentiated the effect of the calcium ionophore A23187. 7. These results indicate that endothelial cells from adrenomedullary capillaries co-express both P2Y- and P2U-purinoceptors. P2Y-purinoceptors are lost in culture with the first passage of the cells. The P2U-purinoceptor subtype present in these cells is insensitive to PPADS and thus similar to that found in aortic endothelial cells." @default.
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• W2007704010 date "1996-11-01" @default.
• W2007704010 modified "2023-10-17" @default.
• W2007704010 title "Co‐existence of P_2Y‐ and PPADS‐insensitive P_2U‐purinoceptors in endothelial cells from adrenal medulla" @default.
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• W2007704010 cites W1965201236 @default.
• W2007704010 cites W1966018982 @default.
• W2007704010 cites W1969210400 @default.
• W2007704010 cites W1972026926 @default.
• W2007704010 cites W1972627505 @default.
• W2007704010 cites W1972767217 @default.
• W2007704010 cites W1973822015 @default.
• W2007704010 cites W1975738308 @default.
• W2007704010 cites W1976033044 @default.
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• W2007704010 cites W1993122272 @default.
• W2007704010 cites W1993360843 @default.
• W2007704010 cites W1994437659 @default.
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• W2007704010 cites W1996024251 @default.
• W2007704010 cites W2000348816 @default.
• W2007704010 cites W2004006472 @default.
• W2007704010 cites W2004516283 @default.
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• W2007704010 cites W2013884843 @default.
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• W2007704010 cites W2023204231 @default.
• W2007704010 cites W2024176341 @default.
• W2007704010 cites W2026398072 @default.
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• W2007704010 doi "https://doi.org/10.1111/j.1476-5381.1996.tb16026.x" @default.
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