Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007730895> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2007730895 endingPage "706" @default.
- W2007730895 startingPage "698" @default.
- W2007730895 abstract "Vasopressin (V2) receptors were solubilized from porcine kidney membranes with the detergent egg lysolecithin. Binding of [3H]vasopressin to the solubilized fraction was rapid, specific, and saturable. The agonist dissociation constants observed in membranes and solubilized fractions were 1.7 +/- 0.3 and 2.3 +/- 0.2 nM, respectively. In competition binding experiments, the solubilized fraction exhibited the same pharmacological profile as the membranes. Chemical crosslinking of [125I]vasopressin to the solubilized fraction followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated a 62-kDa band which was specifically labeled with [125I]vasopressin. Vasopressin binding sites from the solubilized fractions were resolved by gel filtration and ultracentrifugation on a sucrose gradient. In addition, agonist high affinity binding to V2 receptors and its sensitivity to guanine nucleotides were preserved even after solubilization in the absence of prebound agonist prior to solubilization. Addition of guanine nucleotides such as GTP gamma S decreased the specific binding of [3H]arginine vasopressin to these solubilized fractions in a dose-dependent manner, suggesting the solubilization of a V2 receptor-G protein complex. [32P]ADP ribosylation of the solubilized fraction by cholera and pertussis toxins revealed specifically labeled proteins with molecular weights of 42,000-43,000 and 39,000-41,000, respectively, on sodium dodecyl sulfate polyacrylamide gels. Furthermore [35S]GTP gamma S binding to these solubilized fractions was enhanced by vasopressin, confirming that a significant proportion of the vasopressin receptors must be closely coupled to G proteins even when these receptors are solubilized in the absence of agonist. These results are in contrast with those reported for beta, alpha 2 adrenergic and D2 dopaminergic receptor systems, but in agreement with D1 dopaminergic and A1 adenosine receptors. The molecular mechanism responsible for this difference remains to be determined." @default.
- W2007730895 created "2016-06-24" @default.
- W2007730895 creator A5025632362 @default.
- W2007730895 creator A5027525741 @default.
- W2007730895 creator A5035237115 @default.
- W2007730895 creator A5040133857 @default.
- W2007730895 creator A5046832707 @default.
- W2007730895 creator A5077755519 @default.
- W2007730895 date "1989-02-01" @default.
- W2007730895 modified "2023-09-27" @default.
- W2007730895 title "Solubilization of a guanine nucleotide-sensitive form of vasopressin V2 receptors from porcine kidney" @default.
- W2007730895 cites W1013606601 @default.
- W2007730895 cites W1487445062 @default.
- W2007730895 cites W1525252868 @default.
- W2007730895 cites W1527292242 @default.
- W2007730895 cites W1538264730 @default.
- W2007730895 cites W1562393866 @default.
- W2007730895 cites W1592059338 @default.
- W2007730895 cites W1596671720 @default.
- W2007730895 cites W1598210821 @default.
- W2007730895 cites W1629566464 @default.
- W2007730895 cites W1987607664 @default.
- W2007730895 cites W2000271503 @default.
- W2007730895 cites W2017362824 @default.
- W2007730895 cites W2031640329 @default.
- W2007730895 cites W2045884321 @default.
- W2007730895 cites W2073224158 @default.
- W2007730895 cites W2103359865 @default.
- W2007730895 cites W2179950846 @default.
- W2007730895 cites W2427308280 @default.
- W2007730895 cites W4323517774 @default.
- W2007730895 doi "https://doi.org/10.1016/0003-9861(89)90338-x" @default.
- W2007730895 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2521556" @default.
- W2007730895 hasPublicationYear "1989" @default.
- W2007730895 type Work @default.
- W2007730895 sameAs 2007730895 @default.
- W2007730895 citedByCount "16" @default.
- W2007730895 countsByYear W20077308952012 @default.
- W2007730895 crossrefType "journal-article" @default.
- W2007730895 hasAuthorship W2007730895A5025632362 @default.
- W2007730895 hasAuthorship W2007730895A5027525741 @default.
- W2007730895 hasAuthorship W2007730895A5035237115 @default.
- W2007730895 hasAuthorship W2007730895A5040133857 @default.
- W2007730895 hasAuthorship W2007730895A5046832707 @default.
- W2007730895 hasAuthorship W2007730895A5077755519 @default.
- W2007730895 hasConcept C116084860 @default.
- W2007730895 hasConcept C118716 @default.
- W2007730895 hasConcept C170493617 @default.
- W2007730895 hasConcept C181199279 @default.
- W2007730895 hasConcept C185592680 @default.
- W2007730895 hasConcept C2776370428 @default.
- W2007730895 hasConcept C2776885963 @default.
- W2007730895 hasConcept C2778913249 @default.
- W2007730895 hasConcept C2778938600 @default.
- W2007730895 hasConcept C41625074 @default.
- W2007730895 hasConcept C43617362 @default.
- W2007730895 hasConcept C54355233 @default.
- W2007730895 hasConcept C55493867 @default.
- W2007730895 hasConcept C86803240 @default.
- W2007730895 hasConcept C90474776 @default.
- W2007730895 hasConceptScore W2007730895C116084860 @default.
- W2007730895 hasConceptScore W2007730895C118716 @default.
- W2007730895 hasConceptScore W2007730895C170493617 @default.
- W2007730895 hasConceptScore W2007730895C181199279 @default.
- W2007730895 hasConceptScore W2007730895C185592680 @default.
- W2007730895 hasConceptScore W2007730895C2776370428 @default.
- W2007730895 hasConceptScore W2007730895C2776885963 @default.
- W2007730895 hasConceptScore W2007730895C2778913249 @default.
- W2007730895 hasConceptScore W2007730895C2778938600 @default.
- W2007730895 hasConceptScore W2007730895C41625074 @default.
- W2007730895 hasConceptScore W2007730895C43617362 @default.
- W2007730895 hasConceptScore W2007730895C54355233 @default.
- W2007730895 hasConceptScore W2007730895C55493867 @default.
- W2007730895 hasConceptScore W2007730895C86803240 @default.
- W2007730895 hasConceptScore W2007730895C90474776 @default.
- W2007730895 hasIssue "2" @default.
- W2007730895 hasLocation W20077308951 @default.
- W2007730895 hasLocation W20077308952 @default.
- W2007730895 hasOpenAccess W2007730895 @default.
- W2007730895 hasPrimaryLocation W20077308951 @default.
- W2007730895 hasRelatedWork W1973811817 @default.
- W2007730895 hasRelatedWork W1993414773 @default.
- W2007730895 hasRelatedWork W2005039156 @default.
- W2007730895 hasRelatedWork W2005895239 @default.
- W2007730895 hasRelatedWork W2015402980 @default.
- W2007730895 hasRelatedWork W2033116575 @default.
- W2007730895 hasRelatedWork W2144939206 @default.
- W2007730895 hasRelatedWork W2411855751 @default.
- W2007730895 hasRelatedWork W2414761485 @default.
- W2007730895 hasRelatedWork W2417753404 @default.
- W2007730895 hasVolume "268" @default.
- W2007730895 isParatext "false" @default.
- W2007730895 isRetracted "false" @default.
- W2007730895 magId "2007730895" @default.
- W2007730895 workType "article" @default.