Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007806134> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2007806134 abstract "3H-naloxone specific binding was carried out on synaptosomal membranes isolated from basal ganglia of the cat brain. A high- and a low-affinity site with Kd1 = 3.7 nM and Kd2 = 35 nM having B max 1 = 79 pmole/g protein and B max 2 = 224 pmole/g protein were found. The Hill number for the high- and low-affinity sites were, respectively, 1.01 and 0.86. Digitonin and Triton X-100 had an inhibitory effect on the binding at concentrations between 10(-5) and 10(-1)% (w/v). Deoxycholate and Nonidet P-40 also inhibited the binding of 3H-naloxone, but at 10(-4)% produced a 50% enhancement. After the binding to membranes, the 3H-naloxone receptor complex is stable to the action of Triton X-100 and dissociates slowly. In membranes bound with 10 nM 3H-naloxone and then submitted to 0.1-0.2% Triton X-100, in which only the presynaptic membrane disintegrates, the specific radioactivity is decreased. With a more drastic treatment that disintegrates the postsynaptic membrane, the 3H-naloxone binding to synaptosomal membranes is almost completely abolished. These results suggest that opiate receptors may be localized both pre- and postsynaptically in central synapses." @default.
- W2007806134 created "2016-06-24" @default.
- W2007806134 creator A5015046856 @default.
- W2007806134 creator A5080880930 @default.
- W2007806134 creator A5084716960 @default.
- W2007806134 date "1981-05-01" @default.
- W2007806134 modified "2023-10-14" @default.
- W2007806134 title "Action of detergents and pre- and postsynaptic localization of3H-naloxone binding in synaptosomal membranes. A structural approach" @default.
- W2007806134 cites W1775749144 @default.
- W2007806134 cites W1967672967 @default.
- W2007806134 cites W1968422198 @default.
- W2007806134 cites W1971165480 @default.
- W2007806134 cites W1977249450 @default.
- W2007806134 cites W1979385488 @default.
- W2007806134 cites W1982152340 @default.
- W2007806134 cites W1991276891 @default.
- W2007806134 cites W2006247400 @default.
- W2007806134 cites W2013687606 @default.
- W2007806134 cites W2015006483 @default.
- W2007806134 cites W2023692537 @default.
- W2007806134 cites W2025355339 @default.
- W2007806134 cites W2031157126 @default.
- W2007806134 cites W2051304325 @default.
- W2007806134 cites W2084442692 @default.
- W2007806134 cites W2088497455 @default.
- W2007806134 cites W2167773427 @default.
- W2007806134 doi "https://doi.org/10.1002/neu.480120306" @default.
- W2007806134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6268744" @default.
- W2007806134 hasPublicationYear "1981" @default.
- W2007806134 type Work @default.
- W2007806134 sameAs 2007806134 @default.
- W2007806134 citedByCount "6" @default.
- W2007806134 crossrefType "journal-article" @default.
- W2007806134 hasAuthorship W2007806134A5015046856 @default.
- W2007806134 hasAuthorship W2007806134A5080880930 @default.
- W2007806134 hasAuthorship W2007806134A5084716960 @default.
- W2007806134 hasConcept C107824862 @default.
- W2007806134 hasConcept C12554922 @default.
- W2007806134 hasConcept C134018914 @default.
- W2007806134 hasConcept C170493617 @default.
- W2007806134 hasConcept C17077164 @default.
- W2007806134 hasConcept C185592680 @default.
- W2007806134 hasConcept C197341189 @default.
- W2007806134 hasConcept C2776885963 @default.
- W2007806134 hasConcept C2777064678 @default.
- W2007806134 hasConcept C2778750930 @default.
- W2007806134 hasConcept C2778920010 @default.
- W2007806134 hasConcept C2910644744 @default.
- W2007806134 hasConcept C41625074 @default.
- W2007806134 hasConcept C55493867 @default.
- W2007806134 hasConcept C58226133 @default.
- W2007806134 hasConcept C86803240 @default.
- W2007806134 hasConceptScore W2007806134C107824862 @default.
- W2007806134 hasConceptScore W2007806134C12554922 @default.
- W2007806134 hasConceptScore W2007806134C134018914 @default.
- W2007806134 hasConceptScore W2007806134C170493617 @default.
- W2007806134 hasConceptScore W2007806134C17077164 @default.
- W2007806134 hasConceptScore W2007806134C185592680 @default.
- W2007806134 hasConceptScore W2007806134C197341189 @default.
- W2007806134 hasConceptScore W2007806134C2776885963 @default.
- W2007806134 hasConceptScore W2007806134C2777064678 @default.
- W2007806134 hasConceptScore W2007806134C2778750930 @default.
- W2007806134 hasConceptScore W2007806134C2778920010 @default.
- W2007806134 hasConceptScore W2007806134C2910644744 @default.
- W2007806134 hasConceptScore W2007806134C41625074 @default.
- W2007806134 hasConceptScore W2007806134C55493867 @default.
- W2007806134 hasConceptScore W2007806134C58226133 @default.
- W2007806134 hasConceptScore W2007806134C86803240 @default.
- W2007806134 hasLocation W20078061341 @default.
- W2007806134 hasLocation W20078061342 @default.
- W2007806134 hasOpenAccess W2007806134 @default.
- W2007806134 hasPrimaryLocation W20078061341 @default.
- W2007806134 hasRelatedWork W1968655296 @default.
- W2007806134 hasRelatedWork W2007806134 @default.
- W2007806134 hasRelatedWork W2025355339 @default.
- W2007806134 hasRelatedWork W2025904162 @default.
- W2007806134 hasRelatedWork W2058985228 @default.
- W2007806134 hasRelatedWork W2063735777 @default.
- W2007806134 hasRelatedWork W2069638772 @default.
- W2007806134 hasRelatedWork W2415257805 @default.
- W2007806134 hasRelatedWork W2415483486 @default.
- W2007806134 hasRelatedWork W4289953536 @default.
- W2007806134 isParatext "false" @default.
- W2007806134 isRetracted "false" @default.
- W2007806134 magId "2007806134" @default.
- W2007806134 workType "article" @default.